The introduction of a double-channel system for the intrafollicular treatment of cattle

Čech, Svatopluk; Malá, Jana; Indrová, Eva; Lopatářová, M.; Doležel, R.; Dluhosova, H.; Zilka, L.

doi:10.17221/6651-vetmed

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…By using the ultrasound technique we have the opportunity to observe the follicle and CL development without any influence on their physiology (Roelofs et al 2004). Moreover, the ultrasound technique offers additionally the possibility to collect target-oriented samples from follicles by ultrasound-guided follicle aspiration (Cech et al 2013) or from the CL by an ultrasound-guided biopsy (Kot et al 1999). In heifers, as well as in cows, it is described that following sample collection of follicle fluid by follicle aspiration from a preovulatory follicle (after the luteinising hormone surge), development of a CL does occur (Hayashi et al 2006;O'Hara et al 2012).…”

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confidence: 99%

Corpus luteum development and its morphology after aspiration of a preovulatory follicle is related to size and steroid content of the follicle in dairy cows

Vernunft¹,

Weitzel²,

Viergutz³

2013

Vet. Med. - Czech

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Secretion of adequate levels of progesterone from a proper corpus luteum (CL), which develops out of the cells of a healthy preovulatory follicle, is a key-factor for establishment of a pregnancy. The aim of this study was to investigate the relationship between morphological and secretory characteristics of preovulatory follicles and their corresponding corpus luteum with regard to the post-partum period in high-yielding dairy cows. Therefore, ultrasound-guided aspirations of preovulatory follicles were performed repeatedly, using 20 first lactating cows between 26 and 121 days after parturition. Heat was induced with a PGF analogon followed by administration of a GnRH analogon. The dominant follicle was aspirated 21 h after administration of the GnRH analogon. The diameters of the follicles were estimated at aspiration and the morphology of the resulting luteal tissue was examined on day 14 after follicle aspiration using ultrasonographic examinations. Concentrations of progesterone (P 4 ) and 17-beta-oestradiol (E 2 ) were determined in the follicular fluids (FF) and P 4 concentration was estimated at the time of CL examination in plasma. A CL development occurred in 82% after dominant follicle aspiration. The interval of time between parturition and follicle aspiration did not affect the investigated variables. The diameter of the aspirated preovulatory follicle was positively correlated to the cross-section area of the developed luteal tissue (R = 0.60; P < 0.01) as well as to the plasma P 4 concentration on day 14 after follicle aspiration (R = 0.47; P < 0.05). Also, E 2 concentrations in FF were positively correlated to cross-section area of the luteal tissue (R = 0.54; P < 0.05). Comparing the FF of the follicles that gave rise to a CL after aspiration to follicles that did not, both types had comparable P 4 , but the former type harboured higher E 2 concentrations. In conclusion, preovulatory follicle diameter as well as steroid concentrations in the follicular fluid could be used prospectively to identify cows which will have well-developed CLs and high plasma P 4 levels later. On the other hand, CL development after follicle aspiration can be used as a retrospective quality parameter of dominant follicles. These results will help to identify suitable animals for breeding or recipients for embryo transfer.Keywords: post-partum period; ultrasound; fertility; progesterone; oestradiol; follicle fluid List of abbreviations CL = corpus luteum, DM = dry matter, E 2 = 17-beta-oestradiol, FF = follicle fluid, GnRH = gonadotropin realising hormone, P 4 = progesterone, PGF = prostaglandin F2 alfa, TMR = total mixed ration

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confidence: 99%

Corpus luteum development and its morphology after aspiration of a preovulatory follicle is related to size and steroid content of the follicle in dairy cows

Vernunft¹,

Weitzel²,

Viergutz³

2013

Vet. Med. - Czech

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“…The new system enables the aspiration of follicular fluid and subsequent IFT with exactly the same amount of solution when the dead volume was only the volume of the needle (Figure 1). Intrafollicular treatment was performed as previously described (Cech et al 2013). …”

Section: Ift Equipmentmentioning

confidence: 99%

“…The aim of the study was to determine an efficient dose of LH administered into the dominant follicle in heifers treated by deslorelin implants using the recently described double channel device for IFT (Cech et al 2013).…”

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Intrafollicular LH administration in dairy heifers treated with a GnRH agonist

et al. 2013

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ABSTRACT:The aim of this study was to evaluate the effect of intrafollicular treatment (IFT) with different doses of luteinising hormone. Experimental heifers were treated with a single deslorelin implant to desensitise gonadotroph cells of the pituitary gland. Thereafter, follicular development was stimulated by exogenous FSH treatment. Intrafollicular treatment with 10, 5, 1 and 0.01 µg LH was performed on one single follicle while other follicles remained untreated. Human chorionic gonadotrophine (2000 UI) was administered intravenously as a control. Ovulation and development of the corpus luteum occurred after all intrafollicular treatments with 10 and 5 µg LH. After IFT using 1 µg of LH 75% animals (3/4) ovulated. The dose of 0.01 µg was not followed by any ovulation whereas control treatments with hCG were followed by an ovulation of the majority of follicles present in the ovaries. In conclusion, IFT with different doses of LH (greater than 0.01 µg) is capable of inducing ovulation.Keywords: intrafollicular treatment; double channel system; luteinising hormone; deslorelin; cattle Abbreviations CL = corpus luteum, FSH = follicle stimulating hormone, hCG = human chorionic gonadotrophin, IFT = intrafollicular treatment, LH = luteinising hormone, OPU = ovum pick-up, TVFA = ultrasound guided transvaginal follicular aspiration

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A potential role of fibrillin-1 (FBN1) mRNA and asprosin in follicular development in water buffalo

et al. 2022

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The introduction of a double-channel system for the intrafollicular treatment of cattle

Cited by 4 publications

References 15 publications

Corpus luteum development and its morphology after aspiration of a preovulatory follicle is related to size and steroid content of the follicle in dairy cows

Corpus luteum development and its morphology after aspiration of a preovulatory follicle is related to size and steroid content of the follicle in dairy cows

Intrafollicular LH administration in dairy heifers treated with a GnRH agonist

A potential role of fibrillin-1 (FBN1) mRNA and asprosin in follicular development in water buffalo

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