The introduction of poliovirus RNA into cells via lipid vesicles (liposomes)

Wilson, T. M. A.; Papahadjopoulos, Demetrios; Taber, Robert

doi:10.1016/0092-8674(79)90296-4

Cited by 102 publications

(22 citation statements)

References 15 publications

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“…Pellets from the ultracentrifugation of purified coxsackievirus A2 were resuspended in extraction buffer (100 mM-NaC1, 50 mt~-Tris-HC1, 5 mM-EDTA, pH 7.4) and the viral RNA extracted with buffer-saturated redistiiled phenol by a modification of the method of Wilson et al (1979). 0-1 vol.…”

Section: Cell Linesmentioning

confidence: 99%

Eclipse of Coxsackievirus Infectivity: the Restrictive Event for a Non-fusing Myogenic Cell Line

Schultz

Crowell

1983

Journal of General Virology

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SUMMARYCoxsackieviruses A2, A5 and B3 did not replicate in LsCL3-U cells (a non-fusing variant of the rat L s myogenic cell line) although these cells possessed a common receptor for coxsackieviruses A2 and A5, and a different receptor for coxsackievirus B3. The restriction in replication was identified as a block in viral eclipse, since 6 MLiC1 treatment permitted recovery of the coxsackievirus A2 inoculum from L8 CL3-U cells after 2 h at 37 °C, and the cells could be transfected by viral RN A. Cellular fusion which was induced in LsCL3-U cultures by herpes simplex virus type 1 (HF strain) facilitated coxsackievirus A2 and A5 replication. Differentiating myogenic L8 cells acquired full susceptibility to infection concurrently with the appearance of acetylcholine receptors, the muscle-specific isoenzyme of creatine phosphokinase, prominent myotube formation and the acquired capacity of the cells to eclipse virus.

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Section: Cell Linesmentioning

confidence: 99%

Eclipse of Coxsackievirus Infectivity: the Restrictive Event for a Non-fusing Myogenic Cell Line

Schultz

Crowell

1983

Journal of General Virology

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“…New applications followed and led to improve the technique. Poliovirus RNA was encapsulated in liposomes of PS and delivered efficiently to cells in an infectious form (Wilson et al, 1979). A comparison between large unilamellar vesicles (LUVs) and multilamellar vesicles (MLVs) of PS indicates that LUVs deliver their content to cell cytoplasm much more efficiently than MLVs and that LUV-entrapped poliovirus RNA produces infection titers 10 to 100 fold higher than when delivered with other techniques.…”

Section: Some Early Experiments Of Dna Delivery To Cells By Means Of mentioning

confidence: 99%

Neutral Liposomes and DNA Transfection

Pisani¹,

Mobbili²,

Bruni³

2011

Non-Viral Gene Therapy

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“…The encapsulation of plasmid DNA into liposomes (12) and the introduction of poliovirus RNA and SV40 DNA into cells via liposomes (13,14) were reported between 1979 and 1980.…”

Section: Prerequisites For Transfection In Vitromentioning

confidence: 99%

Liposomes as a gene delivery system

Ropert

1999

Braz J Med Biol Res

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Gene therapy is an active field that has progressed rapidly into clinical trials in a relatively short time. The key to success for any gene therapy strategy is to design a vector able to serve as a safe and efficient gene delivery vehicle. This has encouraged the development of nonviral DNA-mediated gene transfer techniques such as liposomes. Many liposome-based DNA delivery systems have been described, including molecular components for targeting given cell surface receptors or for escaping from the lysosomal compartment. Another recent technology using cationic lipids has been evaluated and has generated substantial interest in this approach to gene transfer.

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The introduction of poliovirus RNA into cells via lipid vesicles (liposomes)

Cited by 102 publications

References 15 publications

Eclipse of Coxsackievirus Infectivity: the Restrictive Event for a Non-fusing Myogenic Cell Line

Eclipse of Coxsackievirus Infectivity: the Restrictive Event for a Non-fusing Myogenic Cell Line

Neutral Liposomes and DNA Transfection

Liposomes as a gene delivery system

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