1994
DOI: 10.1002/eji.1830240727
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The invariant chain inhibits presentation of endogenous antigens by a human fibroblast cell line

Abstract: The human fibroblast cell line, M1, expressing the products of transfected DRA and DRB1*0101 genes (M1-DR1) was unable to present intact influenza antigens to a series of DR1-restricted human T cell lines and clones, but was fully able to present synthetic peptides for T cell recognition. In contrast, M1-DR1 cells infected with live influenza virus were recognized by two polyclonal hemagglutinin- or whole virus-specific T cell lines and one of four T cell clones. This difference could not be accounted for simp… Show more

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Cited by 37 publications
(21 citation statements)
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“…No evidence was obtained for any conformational difference between the DR1 molecules expressed at the surface of the M1.DR1 cells and those on B-LCL. Furthermore, the M1.DR1 cells have been shown previously to be fully competent at presenting peptide to DR1-restricted T cells (19). The data did not support the suggestion that the T cell clones raised against the M1.DR1 cells required fibroblast-specific peptides presented in the classical endocytic pathway, in that coexpression of Ii and DM that should have optimized the presentation of such peptides reduced the clones' reactivity.…”
Section: Discussionmentioning
confidence: 63%
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“…No evidence was obtained for any conformational difference between the DR1 molecules expressed at the surface of the M1.DR1 cells and those on B-LCL. Furthermore, the M1.DR1 cells have been shown previously to be fully competent at presenting peptide to DR1-restricted T cells (19). The data did not support the suggestion that the T cell clones raised against the M1.DR1 cells required fibroblast-specific peptides presented in the classical endocytic pathway, in that coexpression of Ii and DM that should have optimized the presentation of such peptides reduced the clones' reactivity.…”
Section: Discussionmentioning
confidence: 63%
“…M1 does not constitutively express DR, Ii, or HLA-DM mRNA (L.L., unpublished data; ref. 19). This was confirmed by flow cyto- metric analysis (data not shown and Fig.…”
Section: M1 Cells Do Not Constitutively Express Dr II or Hla-dm Molmentioning
confidence: 99%
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“…15. In all cases, cell viability was determined by the expression of CD45, and intracellular markers were analyzed within CD45 ϩ cells.…”
Section: Methodsmentioning
confidence: 99%
“…For TAP-1 flow cytometry, dispersed cell preparations were permeabilized before staining [17], and for HLA class I and class II viable cells were stained to detect only the surface molecules. Samples of 5000 cells were analysed on a FACScan using the Lysis II software (both from Becton Dickinson, San Jose, CA).…”
Section: Methodsmentioning
confidence: 99%