The investigation of apelin and apelin receptor expressions in mouse endometrium during peri-implantation period

Balcı, Cemre Nur; Golal, Ezgi; Üstünel, İ̇smail; Acar, Nuray

doi:10.1016/j.aanat.2022.152027

Cited by 3 publications

(5 citation statements)

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“…shown that the apelin system is involved in embryo development, implantation, and placentation in mice (Balci et al, 2023). Thus, our study also suggests that hyperandrogenized-associated deregulation of apelin system in the oviduct could also impair gamete transport and fertilization.…”

Section: Discussionsupporting

confidence: 66%

“…shown to be expressed in the uterine muscle of rats and mice (Anima et al, 2023;Balci et al, 2023;O'Carroll et al, 2000) and in the human uterine tissue apelin inhibits uterine contractility in vitro (Hehir & Morrison, 2012). Therefore, the deregulation of apelin system in the hyperandrogenized condition could also affect the contractility of oviduct.…”

Section: Discussionmentioning

confidence: 99%

“…Apelin system is known to play various physiological roles based on the tissues; it modulates gastric and pancreatic secretion and probably has a protective role in the gastric mucosa against harmful effects (Birsen & İzgüt‐Uysal, 2022; Huang et al, 2019). It has recently been shown that the apelin system is involved in embryo development, implantation, and placentation in mice (Balci et al, 2023). Thus, our study also suggests that hyperandrogenized‐ associated deregulation of apelin system in the oviduct could also impair gamete transport and fertilization.…”

Section: Discussionmentioning

confidence: 99%

“…The immunohistochemical study also showed the presence apelin system in the muscle layer oviduct, which indicates a probable role in oviductal muscle contractility. Apelin has been shown to be expressed in the uterine muscle of rats and mice (Anima et al, 2023; Balci et al, 2023; O'Carroll et al, 2000) and in the human uterine tissue apelin inhibits uterine contractility in vitro (Hehir & Morrison, 2012). Therefore, the deregulation of apelin system in the hyperandrogenized condition could also affect the contractility of oviduct.…”

Section: Discussionmentioning

confidence: 99%

“…Apelin is also an adipokine that binds to G‐protein coupled receptor, APJ for various functions (Chapman et al, 2014). It has been shown that apelin and APJ are expressed in the mice uterus and might be involved in implantation and decidualization (Anima et al, 2023; Balci et al, 2023). To the best of our knowledge, the expression of apelin and APJ has not been investigated in the mice oviduct.…”

Section: Introductionmentioning

confidence: 99%

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Expression and localization of apelin and apelin receptor protein in the oviduct of letrozole‐induced hyperandrogenized mice

Dutta,

Anima,

Riba

et al. 2024

Cell Biology International

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Apelin and its receptor (APJ) are expressed in the reproductive organs of some mammalian females. The function of oviduct has also been suggested to be compromised in the hyperandrogenism condition. However, expression of apelin and APJ has not been shown in the oviduct of hyperandrogenized mice. Thus, the present study has investigated the localization and expression of apelin and APJ in the letrozole‐induced hyperandrogenized mice oviduct. Histomorphometric analysis showed decreased lumen of oviduct in the hyperandrogenized mice. Our results showed elevated expression of APJ and decreased abundance of apelin in the hyperandrogenized mice oviduct. This finding suggests impaired apelin signaling in the oviduct of hyperandrogenized mice. The expression of androgen receptor was upregulated while estrogen receptors were downregulated in the hyperandrogenized mice. The expression of HSP70 was also downregulated along with increased expression of active caspase 3 and BAX and decreased expression of BCL2 in hyperandrogenized mice. Furthermore, the phosphorylation of phospho‐Ser473‐Akt and phospho‐Thr308‐Akt also showed differential levels in the oviduct of hyperandrogenized mice. Whether this differential phosphorylation of Akt was solely due to impaired apelin signaling in the oviduct, remains unclear. Moreover, increased androgen signaling and suppressed estrogen signaling coincides with elevated apoptosis. In conclusion, hyperandrogenized conditions could also impair the gamete transport and fertilization process due to apoptosis in the oviduct. However, further study would be required to unravel the exact role of apelin signaling in the oviduct in relation to apoptosis.

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Section: Discussionsupporting

confidence: 66%