The investigation of bioactive secondary metabolites of the methanol extract of eryngium amethystinum

Vuković, Ljiljana; Vukić, D Milena; Đelić, T Gorica; Kačániová, Miroslava; Cvijović, Mirjana R.

doi:10.5937/kgjsci1840113v

Cited by 22 publications

(13 citation statements)

References 15 publications

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“…The base peak at m / z 493, with the loss of 360 m / z is also in agreement with the presence of secoisolariciresinol [68]. Another peak supporting the identification occurred at m / z 329 [tricin-H] − , corresponding to [M-H-secoisolariciresinol-164] − , which is normally equivalent to the loss of a coumaroyl moiety [69]. Peaks 45 and 47 showed pseudomolecular ions [M-H] − of 691 and 643, respectively.…”

Section: Resultssupporting

confidence: 53%

Puccinellia maritima, Spartina maritime, and Spartina patens Halophytic Grasses: Characterization of Polyphenolic and Chlorophyll Profiles and Evaluation of Their Biological Activities

Faustino

Silva

et al. 2019

Molecules

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Halophytic grasses have been recently targeted as possible sources of nutraceutical and medicinal compounds. Nonetheless, few studies have been conducted on the phytochemistry and biological activities of metabolites produced by these plants. Among these, Spartina maritima (Curtis) Fernald, Spartina patens (Aiton.) Muhl., and Puccinellia maritima (Hudson) Parl. are three halophytic grasses whose chemical composition and bioactivities are unknown. The present work broadens the knowledge on the polyphenolic and chlorophyll composition of these species identifying for the first time hydroxycinnamic acids and their derivatives, flavones, flavonols, lignans, as well as chlorophylls and xantophylls. The extracts were particularly rich in caffeic and ferulic acids as well as in trihydroxymethoxyflavone, apigenin and tricin derivatives. Interestingly, several of the identified compounds are relevant from a medicinal and nutraceutical point of view putting in evidence the potential of these species. Thus, the antioxidant, anti-acetylcholinesterase, antibacterial, and antifungal activities of the polyphenolic extracts were assessed as well as the photophysical properties of the chlorophyll-rich extracts. The results, herein presented for the first time, reinforce the nutritional and the medicinal potential of these halophytic grasses.

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Section: Resultssupporting

confidence: 53%

Puccinellia maritima, Spartina maritime, and Spartina patens Halophytic Grasses: Characterization of Polyphenolic and Chlorophyll Profiles and Evaluation of Their Biological Activities

Faustino

Silva

et al. 2019

Molecules

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“…Likewise, the base peak with m/z 435.09 (C 20 H 18 O 11 ; t R 7.83) and fragments peak at m/z 303.05, 287.05, 183.14, and 153.02 are predicted to be avicularin (quercetin 3- α -L-arabinofuranoside) as spectral figures match with the literature of Santos et al [ 117 ]. Moreover, in our spectra, the base peak with m/z 419.09 (C 20 H 18 O 10 ; t R 8.22) and fragments peak at m/z 287.09 ([(M + H) − 132]) are annotated as kaempferol 3-O- α -L-arabinopyranoside taking reference of Vuković et al [ 118 ].…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic, Antimicrobial, and Molecular Profiling of Selected Medicinal Plants

Aryal

Niraula

Khadayat

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Natural products have been the center of attraction ever since they were discovered. Among them, plant-based natural products were popular as analgesics, anti-inflammatory, antidiabetic, and cosmetics and possess widespread biotechnological applications. The use of plant products as cosmetics and therapeutics is deep-rooted in Nepalese society. Although there are few ethnobotanical studies conducted, extensive research of these valuable medicinal plants has not been a priority due to the limitation of technology and infrastructure. Here, we selected 4 traditionally used medicinal plants to examine their bioactive properties and their enzyme inhibition potential. α-Glucosidase and α-amylase inhibitory activities were investigated using an in vitro model followed up by antioxidant and antimicrobial activities. The present study shows that ethyl acetate fraction of Melastoma melabathrium (IC50 9.1 ± 0.3 µg/mL) and water fraction Acacia catechu (IC50 9.0 ± 0.6 µg/mL) exhibit strong α-glucosidase inhibition. Likewise, the highest α-amylase inhibition was shown by crude extracts of Ficus religiosa (IC50 29.2 ± 1.2 µg/mL) and ethyl acetate fractions of Shorea robusta (IC50 69.3 ± 1.1 µg/mL), and the highest radical scavenging activity was shown by F. religiosa with an IC50 67.4 ± 0.6 µg/mL. Furthermore, to identify the metabolites within the fractions, we employed high-resolution mass spectrometry (LC-HRMS) and annotated 17 known metabolites which justify our assumption on activity. Of 4 medicinal plants examined, ethyl acetate fraction of S. robusta, ethyl acetate fraction of M. melabathrium, and water or ethyl acetate fraction of A. catechu extracts illustrated the best activities. With our study, we set up a foundation that provides authentic evidence to the community for use of these traditional plants. The annotated metabolites in this study support earlier experimental evidence towards the inhibition of enzymes. Further study is necessary to explore the clinical efficacy of these secondary molecules, which might be alternatives for the treatment of diabetes and pathogens.

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“…The product ion of C33 occurred at m/z 285 [M-H-308] − , and the compound was tentatively identified as kaempferol-O-neohesperidoside (Supplementary Figure S14). The fragmentation pattern of the compound was provided by [49]. Peak C22 (m/z 579.1703, C27H31O14 + ) was identified on the positive ion mode as kaempferol-O-dirhamnoside based on the occurrence of ion at m/z 285, which suggests the loss of two deoxyhexose moieties (Supplementary Figure S15).…”

Section: Benzophenonesmentioning

confidence: 99%

Metabolite Profiling of Aquilaria malaccensis Leaf Extract Using Liquid Chromatography-Q-TOF-Mass Spectrometry and Investigation of Its Potential Antilipoxygenase Activity In-Vitro

et al. 2020

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The Aquilaria malaccensis species of the genus Aquilaria is an abundant source of agarwood resin and many bioactive phytochemicals. Recent data regarding the chemical constituents and biological activities of Aquilaria leaves led us to attempt to qualitatively profile the metabolites of Aquilaria malaccensis leaves from a healthy, noninoculated tree through phytochemical screening, GC-MS, and LC/Q-TOF-MS. The present work is also the first to report the antilipoxygenase activity of A. malaccensis leaves from healthy noninoculated tree and investigate its toxicity on oral mucosal cells. A total of 53 compounds were tentatively identified in the extract, some of which have been described in literature as exhibiting anti-inflammatory activity. A number of compounds were identified for the first time in the extract of A. malaccensis leaf, including quercetin, quercetin-O-hexoside, kaempferol-O-dirhamnoside, isorhamnetin-O-hexoside, syringetin-O-hexoside, myricetin, tetrahydroxyflavanone, hesperetin, sissotrin, and lupeol. The antilipoxygenase assay was used to determine the lipoxygenase (LOX) inhibitory potential of the extract, while a WST-1 assay was conducted to investigate the effect of the extract on oral epithelial cells (OEC). The extract implied moderate anti-LOX activity with IC50 value of 71.6 µg/mL. Meanwhile, the cell viability of OEC ranged between 92.55% (10 µg/mL)–76.06% ± (100 µg/mL) upon treatment, indicating some potential toxicity risks. The results attained encourage future studies of the isolation of bioactive compounds from Aquilaria malaccensis leaves, as well as further investigation on the anti-inflammatory mechanisms and toxicity associated with their use.

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The investigation of bioactive secondary metabolites of the methanol extract of eryngium amethystinum

Cited by 22 publications

References 15 publications

Puccinellia maritima, Spartina maritime, and Spartina patens Halophytic Grasses: Characterization of Polyphenolic and Chlorophyll Profiles and Evaluation of Their Biological Activities

Puccinellia maritima, Spartina maritime, and Spartina patens Halophytic Grasses: Characterization of Polyphenolic and Chlorophyll Profiles and Evaluation of Their Biological Activities

Antidiabetic, Antimicrobial, and Molecular Profiling of Selected Medicinal Plants

Metabolite Profiling of Aquilaria malaccensis Leaf Extract Using Liquid Chromatography-Q-TOF-Mass Spectrometry and Investigation of Its Potential Antilipoxygenase Activity In-Vitro

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