The investigation of the antitumoral effect of Cornus mas L in mice with ehrlich solid tumor

Abstract

AIM: Cornus mas L is commonly used due to its anti-infl ammatory, anti-carcinogenic and anti-oxidant properties. In the study, the effects of C. mas L extract on a solid tumor were examined in the Ehrlich solid tumor model developed in Balb/C type mice. METHODS: Ehrlich acid tumor (EAT) cells (1x10 6 EAT cell) from the stock animal were injected subcutaneously (s.c.) through the nape of the mice. Treatment groups of solid tumor-induced animals received 100 mg/kg and 200 mg/kg of C. mas L extract intraperitoneally (i.p.) for 14 days. RESULTS: Tumor volumes and animal weights were found to be statistically signifi cant compared to the control group (p < 0.05). AgNOR staining was performed in tumor tissues. Statistically signifi cant differences were observed between the groups in terms of TAA/NA ratio (p < 0.05). Immunohistochemical and biochemical parameters were also evaluated. An estimation of tumor proliferation of the lung, liver, brain, kidney, testis and tumor antioxidant parameters viz. lipid peroxidation, reduced glutathione (GSH), glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) was made. CONCLUSIONS: Our study showed that the anti-tumor effect of C. mas L in assisted tumor development with EAT cells, was mediated by the enhancement of oxidative stress with multiple mechanisms (Tab. 6, Fig. 12, Ref. 38).

Experimental Cancer Models

“…Seher et al studied the anti-tumoral structure of curcumin on the effect of solid and liquid tumors for Ehrlich ascites [16].…”

Cornelian Cherry (Cornus mas L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays

“…Much attention has been drawn to the cytotoxic, antiproliferative, and thus, anticancer [38,[56][57][58][59][60][61] attributes of C. mas L. Furthermore, the antitumor and anti-inflammatory actions of C. mas L. compounds have been successfully applied in the form of nanoparticle carriers containing the extract itself or its various components [9,[62][63][64][65][66]. Cytotoxic/antiproliferative properties of C. mas L. extracts have been observed (based on the aforementioned studies) with the use of various tumor cell lines, such as the following: MCF-7, SKOV-3, PC-3, HeLa, HepG2, CaCo-2, HT29, CT26, A549.…”

Investigation of the effect of yarrow (Achillea millefolium) on Ehrlich ascites tumor

“…The antitumoral and antioxidant effects of herbal extracts have been tested on many cancer models, one of which is the Ehrlich ascites tumor (EAT) model. EAT first appeared as spontaneous mammary adenocarcinoma in a female mouse and was transformed into experimental tumors after subcutaneous transplantation from mouse to mouse [7].…”