The study aimed to determine whether there are differences in motor skills between school-aged girls who ride and who do not ride, aged 12 to 14. The following motor skills were examined: balance, flexibility, limb speed, leg and body coordination, explosive and repetitive strength.Forty-nine respondents were divided into two subsamples, namely riders n = 26 and non-riders n = 23. To assess motor skills, the following tests were used: the Single and double leg stance test on a balance beam, Overhead stick rotation, the Forward bend twist and touch, Plate tapping and Foot tapping, the Japan test 5 x 4,5 m, 20 lunges with a pass under, the Standing broad jump, 2 kg medicine ball throw from a seated position, Sit-ups, Push-ups, and Squats.The distribution of the results was examined with the Kolmogorov-Smirnov test, and the difference in motor abilities was tested with the Mann-Whitney U test and the independent-samples T-test. The association between horseback riding and motor skills was examined using Pearson's and Spearman's correlation analyses. A significance level of p < 0.05 was accepted. The “Z” value was used to calculate the “r” value, and by calculating Eta squared and applying Cohen's d, the impact of horseback riding on the development of motor skills was analyzed.The results showed that there is a significant difference in motor skills between riders and non-riders. A moderate to strong correlation was found between riding and the examined motor skills. The impact of riding on the development of motor skills was detected ranging from moderate to large.