The investigations of total antioxidant status and biochemical serum profile in thymoquinone-treated rats.

Abstract

Background: This study was planned to determine the dosage and duration of thymoquinone (TQ) at which it demonstrates the optimum effect on the total antioxidant status (TAS) and the biochemical parameters in the blood serum. Materials and Methods: 48 male rats (Wistar-albino) weighing between 200-250 g were used as material. Group 1 (control) (TQ solution 5 mg/kg/day), Group 2 (15 mg/kg/day), Group 3 (30 mg/kg/day), Group 4 (45 mg/kg/day) and Group 5 (60 mg/kg/day) were designated, each containing 8 rats. Different doses of TQ (oral gavage) were administered for four weeks. Results: The TAS levels were determined to be considerably low statistically in all TQ-administered groups in comparison with the control group. It was determined that the serum biochemical parameters exerted a significant effect depending on the TQ doses. Conclusion: As a result, rats administered with TQ orally, at 60 mg/kg dosage, show that the liver and kidney function parameters in particular as different from normal. This brings us to the conclusion that at this dosage, there is reliable biochemical wise but future protective studies in which 30 mg/kg doses can be used safely is encouraged.

Therapeutic effect of thymoquinone against lead-induced testicular histological damage in male Wistar rats

“…The oral dose of TQ (5 mg/kg/day) used in this study has been reported previously in several models of toxicity as an excellent protective daily dose in rats and mice (Al‐Zahrani, Mohany, Kandeal, & Badr, ; Kurt, Dede, & Ragbetli, ). TQ was dissolved in warm tap water (free from Pb) (65°C), and the resulting TQ solution was cooled at room temperature before oral administration.…”

Thymoquinone ameliorates lead-induced suppression of the antioxidant system in rat kidneys

“…After the acclimation period, the rats were randomly divided into four groups of eight animals each and were treated for 5 weeks as follows: the control group received tap water; the Pb group received an aqueous solution containing 2,000 ppm of Pb acetate (0.2% w/v) ( 20 – 22 ); the Pb-TQ group was cotreated with Pb (as in the Pb group) plus TQ (5 mg/kg bw/day); and the TQ group received tap water and was given TQ (5 mg/kg bw/day) ( 23 – 25 ). TQ was administered by gastric tube daily between 8:00 and 9:00 am.…”

The Serum Protein Fractions in Thymoquinone Treated Rats

“…It was noted that serum globulin concentrations of chickens in N. sativa oil diet increased significantly (Bölükbaşı et al, 2009). In a study that determined the effects of TQ application on serum globulin levels, it was found that serum globulin levels increased significantly in the 60 mg/kg/day TQ applied group (p<0.05), however they stayed the same in 30 mg/kg/day TQ applied group (Kurt et al, 2015). It was observed in this study that TQ application did not change the total globulin level as well.…”

“…In a study that examined the effects of TQ application on serum total proteins, it was observed that the total protein levels increased in 60 mg/kg/day applied group (p < 0.05), and did not change in 30 mg/kg/day applied group (Kurt et al, 2015). It was further observed that, although the total protein concentrations relatively decreased in 30 mg/kg/day TQ applied group, but, this result was not important (p > 0.05) in this study.…”

“…There are several studies conducted with different objectives, different dosage and durations of TQ applications. In a study that investigated the effects of TQ applications on serum albumin levels, it was identified that there was no statistical differences between the study and control groups (Kurt et al, 2015). In another study, there was no significant effect with the application of 10 and 20 mg/kg/day oral TQ on albumin levels (El-Barbry et al, 2012).…”