The involvement of CD14 in the activation of human monocytes by peptidoglycan monomers

Muhvić, Damir; El-Samalouti, Volker T.; Flad, Hans‐Dieter; Radošević-Stašić, Biserka; Rukavina, Daniel

doi:10.1080/09629350123956

Cited by 13 publications

(10 citation statements)

References 35 publications

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“…The PGN reduced the binding of the biotinylated LPS to LBP and CD14. PGN of some species is known to bind to LBP and CD14 (Rietschel et al, 1998;Muhvic et al, 2001;Weber et al, 2003). As a result, S. sanguinis PGN may inhibit cytokine expression induced by the LPS of periodontopathogens due to inhibition of LPS binding to LBP and CD14.…”

Section: Discussionmentioning

confidence: 94%

Antagonistic effect of peptidoglycan of Streptococcus sanguinis on lipopolysaccharide of major periodontal pathogens

Lee

2015

J Microbiol.

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Streptococcus sanguinis is often found in subgingival biofilm including periodontopathogens, and is correlated with a delay in colonization by periodontopathogens. However, the effect of S. sanguinis on inflammation induced by periodontopathogens is poorly understood. Thus, this study investigated the effect of S. sanguinis peptidoglycan (PGN) on induction of TNF-α, IL-6, and IL-8 expression by lipopolysaccharide (LPS) of periodontal pathogens. LPS was extracted from Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythia, and PGN was isolated from S. sanguinis. THP-1 cells, a monocytic cell-line, were cotreated with LPS of the periodontal pathogens and S. sanguinis PGN, and then the expression of inflammatory cytokines was analyzed by real-time RT-PCR. To analyze the underlying mechanism, the binding assay of the LPS to CD14 or LPS-binding protein (LBP) was performed in the presence or absence of the PGN after coating recombinant human CD14 and LBP on EIA plate. The PGN inhibited the binding of LPS to CD14 and LBP in a dose-dependent manner. Also, THP-1 cells were co-treated with the LPS in the presence of N-acetylmuramic acid and N-acetylglucosamine, as components of PGN, and the competition binding assay to CD14 and LBP was performed. N-acetylmuramic acid inhibited the induction of inflammatory cytokine expression by LPS and the binding of LPS to CD14 or LBP whereas N-acetylglucosamine did not show such effect. Collectively, the results suggest that S. sanguinis PGN inhibited the cytokine expression induced by the LPS of periodontopathogens due to the inhibition of LPS binding to LBP and CD14. N-acetylmuramic acid of PGN may play a role in inhibition of the LPS binding of periodontopathogens to CD14 and LBP.

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Section: Discussionmentioning

confidence: 94%

Antagonistic effect of peptidoglycan of Streptococcus sanguinis on lipopolysaccharide of major periodontal pathogens

Lee

2015

J Microbiol.

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“…The principal component of the outer membrane of Gram-negative bacteria, lipopolysaccharide (LPS), induces the inflammatory response of an organism (Muhvic et al, 2001). Recognition of LPS by immune cells is the first step in the inflammatory response and, therefore, these cells possess some receptors on their surface for this purpose.…”

mentioning

confidence: 99%

Expression of macrophage CD14 receptor in the course of experimental inflammatory responses induced by lipopolysaccharide and muramyl dipeptide

Sládek¹,

Rysanek²

2008

Vet. Med. - Czech

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AbstrACt:The aim of this study was to determine whether expression of CD14 on macrophages is regulated differently during initiation and resolution of the inflammatory response caused by CD14-dependent (lipopolysaccharide) and CD14-independent (muramyldipeptide) bacterial signals. In cell suspensions from the site of inflammation we observed two types of macrophages: non-vacuolized ( N MAC) and vacuolized ( V MAC) cells. N MAC (monocyte-like cells) were dominant during the early stage of the inflammatory response, whilst V MAC contained phagocytosed apoptotic neutrophils in various stages of digestion. These latter cells were dominant during resolution (particularly at the last time point of 168 h). Intramammary instillation of muramyldipeptide (MDP) and lipopolysaccharide (LPS) resulted in a significant increase in the total count of CD14+ N MAC after 24 h (muramyldipeptide P < 0.01 and lipopolysaccharide P < 0.05) compared to phosphate buffered saline (PBS). During resolution of the inflammatory response, a gradual decrease in the total count of CD14+ N MAC was observed. The difference compared with PBS was significant at 48 h and 72 h after instillation of both bacterial agents (muramyldipeptide: P < 0.05; lipopolysaccharide: P < 0.05). A lower total count of CD14+ V MAC was observed as an effect of MDP and LPS at 24 h after induction (P < 0.05), when compared to PBS. During resolution, the total count of CD14+ V MAC increased. Differences (P < 0.01) were observed at 72 h and 168 h after LPS compared to PBS. We therefore assume that the expression of CD14 on macrophages is not regulated differently during the inflammatory responses caused by CD14-dependent and CD14-independent bacterial signals. On the other hand, the stage of the inflammatory response to MDP and LPS played an important role in the regulation of CD14 expression on macrophages.

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“…100 In human monocytes, the cytokine release induced by the disaccharide-pentapeptide (GlcNAc-MurNAc-Ala-isoGln-meso-DAP-Ala-Ala) was enhanced by pre-incubation with sCD14, while anti-CD14 mAb blocked these effects. 141 MDP was also shown to bind to mCD14, preventing the binding of soluble PGN. 142 In human gingival epithelial cells, CD14 and LBP, but not LBP alone, enhanced MDP-stimulated activation 143 arguing for a role of CD14 as a possible MDP receptor.…”

Section: Cd14mentioning

confidence: 99%

MDP and other muropeptides – direct and synergistic effects on the immune system

Traub¹,

Aulock²,

Hartung³

et al. 2006

J. Endotoxin Res.

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The involvement of CD14 in the activation of human monocytes by peptidoglycan monomers

Cited by 13 publications

References 35 publications

Antagonistic effect of peptidoglycan of Streptococcus sanguinis on lipopolysaccharide of major periodontal pathogens

Antagonistic effect of peptidoglycan of Streptococcus sanguinis on lipopolysaccharide of major periodontal pathogens

Expression of macrophage CD14 receptor in the course of experimental inflammatory responses induced by lipopolysaccharide and muramyl dipeptide

MDP and other muropeptides – direct and synergistic effects on the immune system

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