The involvement of endothelial progenitor cells in tumor angiogenesis

Ribatti, Doménico

doi:10.1111/j.1582-4934.2004.tb00319.x

Cited by 126 publications

(112 citation statements)

References 49 publications

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“…They can participate in vasculogenesis in ischemic tissues, as well as in the maintenance of vascular homeostasis (Khakoo and Finkel, 2005). Emerging evidence indicate that bone marrow-derived circulating EPC can contribute to tumor angiogenesis and growth of certain tumors (Ribatti, 2004). A higher number of EPC has been demonstrated in the bone marrow of patients with active MM than in those with treated MM or with MGUS or in subjects with normal marrow (Dominici et al, 2001), which reflects the increased angiogenic potential in MM.…”

Section: Endothelial Cells Progenitor Cellsmentioning

confidence: 99%

Importance of the bone marrow microenvironment in inducing the angiogenic response in multiple myeloma

2006

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Tumor microenvironment is essential for tumor cell proliferation, angiogenesis, invasion and metastasis through its provision of survival signals, secretion of growth and pro-angiogenic factors, and direct adhesion molecule interactions. This review examines its importance in the induction of an angiogenic response in multiple myeloma (MM). The encouraging results of preclinical and clinical trials in which MM has been treated by targeting the tumor microenvironment are also discussed.

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Section: Endothelial Cells Progenitor Cellsmentioning

confidence: 99%

Importance of the bone marrow microenvironment in inducing the angiogenic response in multiple myeloma

2006

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“…An increase of circulating endothelial cells (CEC) is described in several pathologic conditions that involve vascular injury or instability as myocardial infarction, infectious vasculitis and cancer (Mutin et al, 1999;Mancuso et al, 2001;Woywodt et al, 2003;Beerepoot et al, 2004). These CEC are mostly viable and exhibit still proliferative capacity despite their terminal differentiation (Lin et al, 2000;Mancuso et al, 2001;Ribatti, 2004).…”

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confidence: 99%

Real-time PCR of CD146 mRNA in peripheral blood enables the relative quantification of circulating endothelial cells and is an indicator of angiogenesis

et al. 2005

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Angiogenesis is a fundamental process in tumour growth and metastatic dissemination. Possible surrogate markers for tumour angiogenesis are the amounts of circulating endothelial cells (CEC) in peripheral blood and the plasma concentration of vascular endothelial growth factor (VEGF). We tested the suitability of real-time PCR for CD146, an endothelial cell-specific antigen, to quantify CEC numbers in comparison to a flow cytometry quantification. Real-time PCR of CD146 mRNA showed high sensitivity and linearity for the quantification of cultivated primary endothelial cells added in different amounts to blood samples. Circulating endothelial cell numbers were quantified in peripheral blood samples of breast cancer patients and healthy controls by four-colour flow cytometry analysis and CD146 real-time PCR, and VEGF plasma concentrations were measured by ELISA. The amounts of CEC detected with both methods correlated significantly and CEC numbers were significantly increased in newly diagnosed breast cancer patients compared to healthy controls. Vascular endothelial growth factor concentrations correlated significantly with CEC numbers, but there was no significant difference in VEGF levels between breast cancer patients and healthy controls indicating that VEGF plasma levels cannot be used as surrogate marker for tumour angiogenesis. Taken together, the quantification of CEC by CD146 realtime PCR showed equivalent results to the flow cytometry analysis. Thus, CD146 real-time PCR may be an easy and reliable approach to quantify CEC in peripheral blood samples and could facilitate the integration of CEC measurements in clinical studies exploring the efficacy of antiangiogenic therapies.

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“…17 It is also expressed by CD341, VEGFR-21 endothelial precursor cells, which may home to sites of neovascularization, where they differentiate into endothelial cells and contribute to tumor angiogenesis. 18 Thus, CD133 expression seems also to be linked to angiogenesis.However, data regarding the nature of these brain tumor-initiating cells are diverging, and experimental studies have identified nestin-expressing cells as the cell population susceptible to transforming events in the CNS, 19 while stem cell assays conducted on brain tumor tissue reliably produce nestin expressing tumor spheres. 11 Moreover, tumors within the same histological group often contain subgroups with different gene expression profiles that predispose the patients to clinically different outcomes.…”

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confidence: 99%

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confidence: 99%

CD133 negative glioma cells form tumors in nude rats and give rise to CD133 positive cells

Wang

Sakariassen

Tsinkalovsky

et al. 2007

Intl Journal of Cancer

507

393

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CD133 is a cell surface marker expressed on progenitors of haematopoietic and endothelial cell lineages. Moreover, several studies have identified CD133 as a marker of brain tumor-initiating cells. In this study, human glioblastoma multiforme biopsies were engrafted intracerebrally into nude rats. The resulting tumors were serially passaged in vivo, and monitored by magnetic resonance imaging. CD133 expression was analyzed at various passages. Tumors initiated directly from the biopsies expressed little or no CD133, and showed no contrast enhancement suggesting an intact blood-brain barrier. During passaging, the tumors gradually displayed more contrast enhancement, increased angiogenesis and a shorter survival. Real-time qPCR and immunoblots showed that this was accompanied by increased CD133 expression. Primary biopsy spheroids and xenograft tumors were subsequently dissociated and flow sorted into CD133 negative and CD133 positive cell populations. Both populations incorporated BrdU in cell culture, and expressed the neural precursor marker nestin. Notably, CD133 negative cells derived from 6 different patients were tumorgenic when implanted into the rat brains. For 3 of these patients, analysis showed that the resulting tumors contained CD133 positive cells. In conclusion, we show that CD133 negative glioma cells are tumorgenic in nude rats, and that CD133 positive cells can be obtained from these tumors. Upon passaging of the tumors in vivo, CD133 expression is upregulated, coinciding with the onset of angiogenesis and a shorter survival. Thus, our findings do not suggest that CD133 expression is required for brain tumor initiation, but that it may be involved during brain tumor progression. ' 2007 Wiley-Liss, Inc.Key words: CD133; brain cancer; angiogenesis; cancer stem cell; xenograft At present, there is a search for tumor cell subpopulations that may be responsible for tumor initiation and progression. Such cells have been termed cancer stem cells and are defined by their capacity to self-renew, express stem cell markers and to initiate tumors in vivo. 1,2 Potential cancer stem cells have been identified in leukaemias, 3-5 breast, 6 prostate, 7 bone, 8 colon and brain cancer. [9][10][11][12][13] In some cases, these tumor-initiating cells have been distinguished from the non-tumor-initiating ones based on expression of cell surface markers. For instance, it has been shown that only CD44 1 / CD24 2 /Lineage 2 breast cancer cells are tumorgenic in animals. 6 In malignant brain tumors, CD133 has been suggested to be a cancer stem cell marker 11,14 since only CD133 positive cells from brain tumor biopsy material were able to initiate brain cancer in a mouse model. 14 Prominin-1 (PROM-1), also called CD133, is a protein with several isoforms of unknown physiological or pathological function, and is localized both in the cytoplasm and at the cell surface. 15,16 It is expressed by human neural stem cells and has been proposed to have a function in central nervous system (CNS) development. 17 It is also express...

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The involvement of endothelial progenitor cells in tumor angiogenesis

Cited by 126 publications

References 49 publications

Importance of the bone marrow microenvironment in inducing the angiogenic response in multiple myeloma

Importance of the bone marrow microenvironment in inducing the angiogenic response in multiple myeloma

Real-time PCR of CD146 mRNA in peripheral blood enables the relative quantification of circulating endothelial cells and is an indicator of angiogenesis

CD133 negative glioma cells form tumors in nude rats and give rise to CD133 positive cells

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