The involvement of genes related to bile secretion pathway in rat tooth germ development

Yang, Jun; Lu, Xi; Liu, Shangfeng; Zhao, Shouliang

doi:10.1007/s10735-020-09861-0

Cited by 8 publications

(8 citation statements)

References 30 publications

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“…The tooth morphogenesis is triggered by the sequential and reciprocal interactions between ectomesenchyme generated from the cranial neural crest and dental epithelium (Jussila and Thesleff, 2012;Bakhit et al, 2018). The dynamic histological observation of this study revealed that the rat molar germs entered the early cap stage at E14.5 d, the cap stage and early bell stage at E16.5 d, and the bell stage at E18.5 d. The morphogenesis of molar cusps was completed at PN4 d. These results were consistent with that of previous studies (Taniguchi et al, 1999;Yang et al, 2020). Interestingly, the separations between pre-odontoblast and pre-ameloblast layers were found to occur in all the E18.5 d rat first molar, implying a significant decrease in the cell adhesion between odontoblasts and ameloblasts at this stage.…”

Section: Discussionsupporting

confidence: 92%

A potential role of p75NTR in the regulation of circadian rhythm and incremental growth lines during tooth development

Yuan

Xie

et al. 2022

Front. Physiol.

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Objective: Tooth morphogenesis and the formation of hard tissues have been reported to be closely related to circadian rhythms. This study investigates the spatiotemporal expression and relationship of p75NTR with core clock genes, mineralization-related or odontogenesis-related genes, and aims to derive the potential role of p75NTR in regulating circadian rhythm and incrementality growth line formation during tooth development.Materials and methods: The dynamic morphology of the rat dental germ was observed at seven stages (E14.5 d, E16.5 d, E18.5 d, P.N. 4 d, P.N. 7 d, P.N. 10 d, and P.N. 15 d). Next, the expressions of p75NTR and other target factors were traced. The ectomesenchymal stem cells (EMSCs) were isolated from the E18.5d rat dental germs and synchronized using 50% of fetal bovine serum. Then, they were cultured in light/light (L.L.), dark/dark (D.D.), and light/dark (L.D.) conditions for 48 h. The total RNA was collected every 4 h, and the circadian rhythm dynamics of target factors were observed. To reveal the mechanism further, p75NTR was down-regulated in p75NTRExIII−/− mice and up-regulated in immortalized mouse dental apical papilla progenitor cells. The change tendencies of other target factors were also detected.Results: The clock genes Bmal1, Clock, Per1, and Per2 were all expressed in tooth germs before the formation of dental hard tissues and demonstrated a regular oscillating expression pattern in EMSCs from dental germs. Their expression was affected by the L.D. stimulus, and most of them were promoted by D.D. conditions. p75NTR presented a similar expression pattern and a positive or negative relationship with most clock genes, mineralization-related and odontogenesis-related factors, such as brain and muscle ARNT-like protein-1 (Bmal1), runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), MSH-like 1 (MSX1), dentin matrix acidic phosphoprotein 1 (Dmp1), and dentin sialophosphoprotein (Dspp). Moreover, the arrangement, morphology, and even boundary in pre-odontoblast/pre-ameloblast layers were disordered in the p75NTRExIII−/− mice.Conclusion: Circadian rhythm was found to affect tooth development. p75NTR might play a crucial role in regulating clock genes in the mineralization and formation of the dental hard tissues. p75NTR is actively involved in the odontoblast-ameloblast junction and cell polarity establishment during tooth morphogenesis.

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Section: Discussionsupporting

confidence: 92%

A potential role of p75NTR in the regulation of circadian rhythm and incremental growth lines during tooth development

Yuan

Xie

et al. 2022

Front. Physiol.

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“…The mislocalization of CFTR in mouse bile duct cells makes mice present a cholestatic phenotype ( Fiorotto et al, 2016 ). CFTR located in the bile secretion pathway is involved in the regulation of pH of rat ameloblasts, and then in the development of tooth germ ( Yang et al, 2020 ). CFTR may be involved in the formation of calcification, leading to the formation of GD.…”

Section: Discussionmentioning

confidence: 99%

Association Study Between Polymorphic Loci in Cholesterol Metabolism Pathway and Gallstone in the Tibetan Population

Chen

Zhang

et al. 2022

Front. Genet.

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Background: The incidence of gallstones in the Tibetan population is increasing rapidly. Previous studies indicated that genetic variation located in the cholesterol metabolism pathway may be associated with the incidence of gallstones.Methods: By recruiting 132 Tibetan gallstone patients and 52 normal Tibetan controls, we performed next-generation sequencing for 508 genes in the cholesterol metabolism pathway. Additionally, by integrating the sequence data of 41 normal Tibetan subjects in the public database, we finally obtained 93 normal Tibetan controls. Single nucleotide polymorphisms (SNPs) calling were performed by using the GATK pipeline. The quality control criteria for SNPs were: missing rate <0.05; minor allele frequency (MAF) > 0.01; and p value >0.001 in the Hardy-Weinberg Equilibrium (HWE) test. To eliminate the influence of population heterogeneity, Principal Component Analysis (PCA) was carried out by using the smartpca software. Association analyses were performed by Plink software. Multiple tests were adjusted by the false discovery rate (FDR) method.Results: A total of 2,401 SNPs were obtained by analyzing 508 genes, and 2,011 SNPs left after quality control. After adjusting the eigen vectors, we found that 10 SNPs (SNV05997, rs80145081, rs80005560, rs79074685, rs748546375, rs201880593, rs142559357, rs750769471, rs869789 and rs4072341) were significantly associated with gallstone. Subsequently, by comparing the case group with our control group and the public database control group separately, we further found that the SNP rs869789 was consistently significantly associated with gallstone (p = 9.04 × 10–3 in cases vs. our controls and 5.73 × 10–3 in cases vs. public controls, respectively).Conclusion: By systematically analyzed SNPs in the cholesterol metabolism pathway, we identified one polymorphic locus rs869789 significantly associated with the pathogenesis of gallstone in the Tibetan population. This study will provide clue for further mechanism study of gallstone in the Tibetan population.

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“…116 Bile secretion pathway SLC2A1, SLC4A4, ADCY5, ATP1B1, SLC10A1, ABCC3 Expressed in tooth germ odontoblasts. 117 Other related genes IFT140 IFT140 is essential in promoting dentin formation and reparation. 118 EphrinB1, EphB2 Regulates odontogenic differentiation and the early stages of tooth injury.…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have shown that this signaling pathway, ostensibly unrelated to teeth, is involved in tooth embryo development, and tooth formation involves strict genetic control procedures. 117 Therefore, exploring the gene network system regulating tooth development has a very positive practical significance in the study of tooth tissue regeneration and the prevention and treatment of tooth abnormalities. The early bell stage is the initial phase of odontoblast formation and dentin matrix deposition in the tooth development process.…”

Section: Introductionmentioning

confidence: 99%

“…RNA sequencing and differential gene analysis of rat tooth germ samples at the cap and early bell stages showed that the bile secretion pathway was the most significantly different between the cap and bell stages among related signaling pathway during development, which mainly included SLC10A1, SLC2A1, SLC4A4, ADCY5, ATP1B1 and ABCC3. 117 …”

Section: Introductionmentioning

confidence: 99%

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The Genes Involved in Dentinogenesis

et al. 2022

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The development and repair of dentin are strictly regulated by hundreds of genes. Abnormal dentin development is directly caused by gene mutations and dysregulation. Understanding and mastering this signal network is of great significance to the study of tooth development, tissue regeneration, aging, and repair and the treatment of dental diseases. It is necessary to understand the formation and repair mechanism of dentin in order to better treat the dentin lesions caused by various abnormal properties, whether it is to explore the reasons for the formation of dentin defects or to develop clinical drugs to strengthen the method of repairing dentin. Molecular biology of genes related to dentin development and repair are the most important basis for future research.

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The involvement of genes related to bile secretion pathway in rat tooth germ development

Cited by 8 publications

References 30 publications

A potential role of p75NTR in the regulation of circadian rhythm and incremental growth lines during tooth development

A potential role of p75NTR in the regulation of circadian rhythm and incremental growth lines during tooth development

Association Study Between Polymorphic Loci in Cholesterol Metabolism Pathway and Gallstone in the Tibetan Population

The Genes Involved in Dentinogenesis

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