The involvement of hydrogen peroxide in the production of volatile halogenated compounds by Meristiella gelidium

Collén, Jonas; Ekdahl, Anja; Abrahamsson, Katarina; Pedersén, Marianne

doi:10.1016/s0031-9422(00)89637-5

Cited by 89 publications

(55 citation statements)

References 30 publications

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“…Other studies report increased bromocarbon production with the addition of H 2 O 2 (Collén et al, 1994;Küpper et al, 2013) or decreased production with the addition of photosynthesis inhibitors (Goodwin et al, 1997). Methyl halides, which do not scavenge H 2 O 2 , were not affected by light in the Collén et al (1994) study.…”

Section: E C Leedham Elvidge Et Al: the Effect Of Desiccation On Tmentioning

confidence: 60%

“…Carpenter and Liss, 2000;Gschwend et al, 1984;Küpper et al, 2013;Leedham et al, 2013). It is believed that organic and inorganic halides, in their role as antioxidants, may play a role in mitigating ROS damage (Collén et al, 1994) and therefore the macroalgal adaptation to tidal exposure. In several incubation experiments, production of polyhalogenated compounds was enhanced in the light compared to the dark -evidence that halocarbon emissions could be linked to ROS production during photosynthesis (Collén et al, 1994;Keng et al, 2013;Klick, 1993;Nightingale et al, 1995;Pedersén et al, 1996).…”

Section: E C Leedham Elvidge Et Al: the Effect Of Desiccation On Tmentioning

confidence: 99%

“…It is believed that organic and inorganic halides, in their role as antioxidants, may play a role in mitigating ROS damage (Collén et al, 1994) and therefore the macroalgal adaptation to tidal exposure. In several incubation experiments, production of polyhalogenated compounds was enhanced in the light compared to the dark -evidence that halocarbon emissions could be linked to ROS production during photosynthesis (Collén et al, 1994;Keng et al, 2013;Klick, 1993;Nightingale et al, 1995;Pedersén et al, 1996). Other studies report increased bromocarbon production with the addition of H 2 O 2 (Collén et al, 1994;Küpper et al, 2013) or decreased production with the addition of photosynthesis inhibitors (Goodwin et al, 1997).…”

Section: E C Leedham Elvidge Et Al: the Effect Of Desiccation On Tmentioning

confidence: 99%

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The effect of desiccation on the emission of volatile bromocarbons from two common temperate macroalgae

et al. 2015

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Abstract. Exposure of intertidal macroalgae during low tide has been linked to the emission of a variety of atmospherically-important trace gases into the coastal atmosphere. In recent years, several studies have investigated the role of inorganic iodine and organoiodides as antioxidants and their emission during exposure to combat oxidative stress, yet the role of organic bromine species during desiccation is less well understood. In this study the emission of dibromomethane (CH 2 Br 2 ) and bromoform (CHBr 3 ) during exposure and desiccation of two common temperate macroalgae, Fucus vesiculosus and Ulva intestinalis, is reported. Determination of the impact exposure may have on algal physiological processes is difficult as intertidal species are adapted to desiccation and may undergo varying degrees of desiccation before their physiology is affected. For this reason we include comparisons between photosynthetic capacity (F v /F m ) and halocarbon emissions during a desiccation time series. In addition, the role of rewetting with freshwater to simulate exposure to rain was also investigated. Our results show that an immediate flux of bromocarbons occurs upon exposure, followed by a decline in bromocarbon emissions. We suggest that this immediate bromocarbon pulse may be linked to volatilisation or emissions of existing bromocarbon stores from the algal surface rather than the production of bromocarbons as an antioxidant response.

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Section: E C Leedham Elvidge Et Al: the Effect Of Desiccation On Tmentioning

confidence: 60%

Section: E C Leedham Elvidge Et Al: the Effect Of Desiccation On Tmentioning

confidence: 99%

Section: E C Leedham Elvidge Et Al: the Effect Of Desiccation On Tmentioning

confidence: 99%

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The effect of desiccation on the emission of volatile bromocarbons from two common temperate macroalgae

et al. 2015

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“…Studies exposing red and brown seaweeds to 1-2 mM H 2 O 2 showed enhanced CHBr 3 production in the light (Wever et al 1991;Collen et al 1994;Pedersen et al 1996;Sundstrom et al 1996). These seaweeds contain many more cell layers than the two-cell layer thick Ulva and also have thicker cell walls.…”

Section: Effectormentioning

confidence: 99%

Physiological constraints on bromoform (CHBr₃) production by Ulva lactuca (Chlorophyta)

Manley

Barbero

2001

Limnology & Oceanography

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Physiological factors affecting bromoform (CHBr 3 ) production by Ulva lactuca were investigated using metabolic inhibitors and presumed substrates of bromoperoxidase (BrPO). The metabolic inhibitors were used at a verified physiologically active concentration. Bromoform production was nearly tripled in the light (376 Ϯ 92 pg cm Ϫ2 h Ϫ1 ) compared to the dark (114 Ϯ 70 pg cm Ϫ2 h Ϫ1 ), was inhibited in the light in the presence of the photosynthetic inhibitor DCMU, and was inhibited in the dark in the presence of mitochondrial respiratory inhibitor rotenone. Removal of H 2 O 2 from seawater (treatment with catalase) decreased CHBr 3 production in the light and dark. Addition of H 2 O 2 to incubations at either 1.0 mM or 100 M significantly decreased CHBr 3 production in the light and inhibited photosynthesis. In the dark, CHBr 3 production was decreased and respiration inhibited in the presence of 1 mM H 2 O 2 ; CHBr 3 production was enhanced and respiration was not affected in the presence of 100 M H 2 O 2 . Removal of dissolved organic matter (DOM) from seawater decreased CHBr 3 production, as did the addition of alternative BrPO substrates. These results suggest the presence of an extracellular and intracellular BrPO that protects the alga from both internally produced and externally present H 2 O 2 . The results show that H 2 O 2 produced as a result of photosynthetic and respiratory electron transport, presumably by superoxide dismutase, is available to BrPO for bromination, and that the bromination of an unidentified metabolite (presumably ␤-keto acids) and a component of DOM leads to the production of volatile polybromomethanes.Bromoform (CHBr 3 ) is an important source of tropospheric organobromine that can penetrate into the stratosphere in chemically significant amounts, in spite of its relatively short atmospheric lifetime (Sturges et al. 2000). Over the past decade, marine algal production of bromoform and other polyhalomethanes have been repeatedly demonstrated and it has been estimated that marine algae produce approximately 70% of global bromoform (Carpenter and Liss 2000).Bromoperoxidase (BrPO) has been identified as the brominating enzyme. Its activity requires H 2 O 2 , bromide ion, and a suitable organic substrate. Theiler et al. (1978) proposed a mechanism for polybromomethane biosynthesis that involved the bromination by BrPO of ␤-keto acids (e.g., 3-oxooctanoic acid) and cyclic ␤-diketones that are found in marine algae. Bromination produces an intermediate brominated heptanone that yields CH 2 Br 2 , CHBr 3 , and 1-pentyl bromide on hydrolysis. Wever et al. (1991) proposed that CHBr 3 is produced in situ from components of DOM (dissolved organic matter) that are brominated by a reaction with hypobromous acid (HOBr), which itself is a product of BrPO activity.In vivo bromination of phenol red by Ascophyllum nodusum was shown to be enhanced in the presence of exogenous H 2 O 2 (Wever et al. 1991). Similarly, bromoform (CHBr 3 ) production was enhanced with the addition of H 2 O 2 1 C...

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“…They are thought to be responsible for the production of a variety of halometabolites identified in marine algae, including environmentally significant volatile halocarbons such as bromoform [2]. Vanadiumdependent haloperoxidase was first identified in the brown macroalga Ascophylum nodosum [3].…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

Purification, crystallisation and preliminary X‐ray analysis of the vanadium‐dependent haloperoxidase from Corallina officinalis

Rush¹,

Willetts²,

Davies³

et al. 1995

FEBS Letters

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understanding of the multimeric arrangements of the protein subunits and the role of vanadium in the catalytic action. One property of the vanadium cofactor in haloperoxidases is that it can be removed at low pH in the presence of EDTA, rendering the enzyme inactive. Incubation of the enzyme at neutral pH with vanadate restores activity. This has led to the suggestion, which is confirmed by spectroscopic studies, that the vanadium is in the valance state 5+ (3d °) [14].

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The involvement of hydrogen peroxide in the production of volatile halogenated compounds by Meristiella gelidium

Cited by 89 publications

References 30 publications

The effect of desiccation on the emission of volatile bromocarbons from two common temperate macroalgae

The effect of desiccation on the emission of volatile bromocarbons from two common temperate macroalgae

Physiological constraints on bromoform (CHBr₃) production by Ulva lactuca (Chlorophyta)

Purification, crystallisation and preliminary X‐ray analysis of the vanadium‐dependent haloperoxidase from Corallina officinalis

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The involvement of hydrogen peroxide in the production of volatile halogenated compounds by Meristiella gelidium

Cited by 89 publications

References 30 publications

The effect of desiccation on the emission of volatile bromocarbons from two common temperate macroalgae

The effect of desiccation on the emission of volatile bromocarbons from two common temperate macroalgae

Physiological constraints on bromoform (CHBr3) production by Ulva lactuca (Chlorophyta)

Purification, crystallisation and preliminary X‐ray analysis of the vanadium‐dependent haloperoxidase from Corallina officinalis

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Physiological constraints on bromoform (CHBr₃) production by Ulva lactuca (Chlorophyta)