The involvement of NF-κB/P38 pathways inScutellaria baicalensisextracts attenuating ofEscherichia coliK88-induced acute intestinal injury in weaned piglets

Abstract

The present study was carried out to evaluate the effect of dietary supplementation of Scutellaria baicalensis extracts (SBE) on intestinal health in terms of morphology, barrier integrity and immune responses in weaned piglets challenged with Escherichia coli K88. A total of seventy-two weaned piglets were assigned into two groups to receive a basal diet without including antibiotic additives or the basal diet supplemented 1000 mg SBE/kg diet for 14 d. On day 15, twelve healthy piglets from each group were selected to expose to oral administration of either 10 ml 1 × 109 colony-forming units of E. coli K88 or the vehicle control. After 48 h of E.coli K88 challenge, blood was sampled, and then all piglets were killed humanely for harvesting jejunal and ileal samples. Dietary supplementation of SBE significantly decreased diarrhoea frequency and improved feed conversion ratio (P < 0·05). SBE supplementation to E.coli K88-challenged piglets improved villous height and villous height/crypt depth (P < 0·05), recovered the protein expression of occludin and zonula occludens-2 in both the jejunum and ileum (P < 0·05), and mitigated the increases in plasma IL-1β, TNF-α, IL-6, IgA and IgG (P < 0·05). Meanwhile, dietary SBE effectively inhibited the stimulation of NF-κB, P38 and TNF-α as well as IL-1β in the small intestine of piglets challenged by E. coli K88 and prevented the activation of NF-κB/P38 signalling pathways (P < 0·05). Collectively, SBE supplementation can potently attenuate diarrhoea in weaning piglets and decrease inflammatory cytokine expressions through inhibiting the NF-κB and P38 signalling pathways.

Pyrroloquinoline Quinone Alleviates Jejunal Mucosal Barrier Function Damage and Regulates Colonic Microbiota in Piglets Challenged With Enterotoxigenic Escherichia coli

“…On day 14 of the experiment, piglets in the CTRL + K88 and PQQ + K88 groups were gavaged with 10 mL 1 × 10 9 CFU/mL of ETEC K88 (serotype O149; China Institute of Veterinary Drug Control, Beijing, China), and piglets in the CTRL and PQQ groups were gavaged with 10 mL of PBS. Body temperature was recorded at 0, 4, 12, 24, and 48 h after ETEC K88 challenge, and piglets were euthanized for sampling at 48 h. The dose of ETEC K88 and the timeline to euthanize pigs were based on a previous study conducted in our lab (Huang et al, 2019).…”

Nano chitosan–zinc complex improves the growth performance and antioxidant capacity of the small intestine in weaned piglets

“…The diarrhoea incidence was calculated according to the following formula: diarrhoea rate (%) = 100 × (number of piglets with diarrhoea × number of diarrhoea days) ÷ (total number of piglets × number of experiment days). After the feeding trial, at 8:00 am on the 15 th day, six piglets were randomly selected from each treatment group for oral ingestion of ETEC K88 at a concentration of approximately 1 × 10 9 colony-forming units (CFU)/mL in 10 mL of phosphate-buffered saline (PBS) per piglet according to the methods of Huang et al (5) . After 48 h of ETEC K88 challenge, all challenged piglets were slaughtered and sampled.…”

WITHDRAWN: Effect of Hermetia Illucens Larvae on Growth Performance of Weaned Piglets and Diarrhea Caused by Escherichia Coli K88

“…The villi of the small intestine help to increase the absorption area of the small intestine. Severe damage in the ileum villi of the C+K88 group indicated that ETEC K88 is detrimental to the villus structure of the small intestine [33,34]. Villi are an important part of the digestive tract, and the absorption capacity of the small intestine relies on the height of the villi [35].…”