The Involvement of NFAT Transcriptional Activity Suppression in SIRT1-Mediated Inhibition of COX-2 Expression Induced by PMA/Ionomycin

Abstract

SIRT1, a class III histone deacetylase, acts as a negative regulator for many transcription factors, and plays protective roles in inflammation and atherosclerosis. Transcription factor nuclear factor of activated T cells (NFAT) has been previously shown to play pro-inflammatory roles in endothelial cells. Inhibition of NFAT signaling may be an attractive target to regulate inflammation in atherosclerosis. However, whether NFAT transcriptional activity is suppressed by SIRT1 remains unknown. In this study, we found that SIRT1 suppressed NFAT-mediated transcriptional activity. SIRT1 interacted with NFAT, and the NHR and RHR domains of NFAT mediated the interaction with SIRT1. Moreover, we found that SIRT1 primarily deacetylated NFATc3. Adenoviral over-expression of SIRT1 suppressed PMA and calcium ionophore Ionomycin (PMA/Io)-induced COX-2 expression in human umbilical vein endothelial cells (HUVECs), while SIRT1 RNAi reversed the effects in HUVECs. Moreover, inhibition of COX-2 expression by SIRT1 in PMA/Io-treated HUVECs was largely abrogated by inhibiting NFAT activation. Furthermore, SIRT1 inhibited NFAT-induced COX-2 promoter activity, and reduced NFAT binding to the COX-2 promoter in PMA/Io-treated HUVECs. These results suggest that suppression of NFAT transcriptional activity is involved in SIRT1-mediated inhibition of COX-2 expression induced by PMA/Io, and that the negative regulatory mechanisms of NFAT by SIRT1 may contribute to its anti-inflammatory effects in atherosclerosis.

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“…Abbreviation: VEGF, vascular endothelial cell growth factor. and VSMCs in CVDs [150][151][152]. As thus, β-hydroxybutyrate-mediated protection of vascular cell senescence may be partially mediated by p53 β-hydroxybutyrylation.…”

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“…SIRT1 is a NAD + dependent histone/protein deacetylase which has been implicated in a wide array of cellular events including starvation, inflammation, oxidative stress and senescence [21]. SIRT1 mediates these cellular events in part through directly deacetylating several transcription factors such as p53 [22], FOXOs [23], Egr-1 [24], p65 [25], and NFATc3 [26]. Among these transcription factors, Rel family members p65 and NFATc3 were identified to be negatively regulated by direct SIRT1 dependent deacetylation [25,26].…”

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