EXCLI Journal; 21:Doc935; ISSN 1611-2156 2021
DOI: 10.17179/excli2021-3706
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The involvement of small heat shock protein in chemoresistance in ovarian cancer - in vitro study

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Cited by 6 publications
(6 citation statements)
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References 58 publications
(68 reference statements)
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“…Whole blood from UM patients and healthy donors was collected using S-Monovette ® Serum Gel tubes with clotting activator and separating gel (Sartstedt, Newton, NC, United States) and centrifuged at 2500 x g for 10 min to isolate the serum, which was aliquoted and frozen at -80 °C. Exosomes were isolated using several centrifugation steps as previously described ( Wróblewska et al, 2021 ; Wyciszkiewicz et al, 2021 ). First, thawed serum was centrifuged for 30 min at 500 x g. Further, to get rid of larger debris and apoptotic bodies, the supernatant was centrifuged for 45 min at 12,000 x g. Then, it was filtered using 0.2/0.8 µm Acrodisc ® PF syringe filters (Pall, Port Washington, NY, United States) and transferred to Amicon ® Ultra-15 Centrifugal Filter Unit with a 100 kDa cut-off (Merck KGaA, Darmstadt, Germany), filled with Phosphate-Buffered Saline (PBS) (Biowest, Nuaillé, France) and centrifuged for 15 min at 5000 rpm to enrich the exosomal fraction and to decrease the viscosity of the sample.…”
Section: Methodsmentioning
confidence: 99%
“…Whole blood from UM patients and healthy donors was collected using S-Monovette ® Serum Gel tubes with clotting activator and separating gel (Sartstedt, Newton, NC, United States) and centrifuged at 2500 x g for 10 min to isolate the serum, which was aliquoted and frozen at -80 °C. Exosomes were isolated using several centrifugation steps as previously described ( Wróblewska et al, 2021 ; Wyciszkiewicz et al, 2021 ). First, thawed serum was centrifuged for 30 min at 500 x g. Further, to get rid of larger debris and apoptotic bodies, the supernatant was centrifuged for 45 min at 12,000 x g. Then, it was filtered using 0.2/0.8 µm Acrodisc ® PF syringe filters (Pall, Port Washington, NY, United States) and transferred to Amicon ® Ultra-15 Centrifugal Filter Unit with a 100 kDa cut-off (Merck KGaA, Darmstadt, Germany), filled with Phosphate-Buffered Saline (PBS) (Biowest, Nuaillé, France) and centrifuged for 15 min at 5000 rpm to enrich the exosomal fraction and to decrease the viscosity of the sample.…”
Section: Methodsmentioning
confidence: 99%
“…Moreover, miRNAs can modulate gene expression in CAFs, promoting a pro-tumorigenic phenotype characterized by increased secretion of growth factors, ECM remodeling, and enhanced migratory properties. The crosstalk between CCs and CAFs via exosomal miRNAs can contribute to immune evasion and angiogenesis within the TME ( Wróblewska et al, 2021 ; Wyciszkiewicz et al, 2021 ). CAF-derived exosomes often mediate tumorigenesis since they carry, i.a., TGF-β activating SMAD pathway, CD81 participating in the Wnt pathway, or CD9 participating in MMP2 signaling, leading to ECM remodeling ( Peng et al, 2022 ).…”
Section: Cross-talk Of Cafs and Tme Cellsmentioning
confidence: 99%
“…Thus, although a number of drug-candidates has been tested so far, none of them has been introduced into clinics [ 14 – 16 ]. Still, it is believed that finding an agent which targets a key resistance pathway and enhances cell response to the platinum-based treatments, would finally provide a long-anticipated therapeutic success in patients with poor prognosis.…”
Section: Introductionmentioning
confidence: 99%