The IPCS Collaborative Study on Neurobehavioral Screening Methods

Moser, Virginia C.; Becking, G.C.; MacPhail, Robert C.; Kulig, B.M.

doi:10.1093/toxsci/35.2.143

Cited by 42 publications

(58 citation statements)

References 18 publications

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“…A standard assessment for the effects (or lack of effects) of toxins and suspected toxins is the measurement of any changes in behavior under standard conditions (Moser et al, 1997). EthoVision is used by a number of groups for these measurements.…”

Section: Toxicologymentioning

confidence: 99%

EthoVision: A versatile video tracking system for automation of behavioral experiments

Noldus

Spink

Tegelenbosch

2001

Behavior Research Methods, Instruments, & Computers

601

446

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Section: Toxicologymentioning

confidence: 99%

EthoVision: A versatile video tracking system for automation of behavioral experiments

Noldus

Spink

Tegelenbosch

2001

Behavior Research Methods, Instruments, & Computers

601

446

View full text Add to dashboard Cite

“…The results of the experiments related to the measurement of cyclosarin-induced neurotoxicity at 24 hours following cyclosarin poisoning are divided into three parts (activity and neuromuscular measures, sensorimotor and excitability measures and autonomic measures) (18) and summarized in Table 2a-c. Three non-treated cyclosarinpoisoned rats and cyclosarin-poisoned rats treated with atropine in combination with K203, two cyclosarin-poisoned rat treated with atropine in combination with K156 or obidoxime and one cyclosarin-poisoned rat treated with atropine in combination with HI-6 died within 24 hours of cyclosarin administration.…”

Section: Resultsmentioning

confidence: 99%

“…Some of them are scored (Tab. 1), the others are measured in absolute units (3,4,18). The first evaluation was obtained when cyclosarin-poisoned rats were in the home cage.…”

Section: Methodsmentioning

confidence: 99%

A Comparison of the Neuroprotective Efficacy of Newly Developed Oximes (K156, K203) and Currently Available Oximes (Obidoxime, HI-6) in Cyclosarin-poisoned Rats

Kassa

Karasová

Tesarova

et al. 2008

Acta Med. (Hradec Kralove, Czech Repub.)

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Summary:The neuroprotective effects of newly developed oximes (K156, K203) and currently available oximes (obidoxime, HI-6) in combination with atropine in rats poisoned with cyclosarin were studied. The cyclosarin-induced neurotoxicity was monitored using a functional observational battery 24 hours after cyclosarin challenge. The results indicate that a newly developed oxime K156 is able to counteract slightly cyclosarin-induced neurotoxicity while another newly developed oxime K203 is completely ineffective in reducing cyclosarin-induced neurotoxic signs and symptoms. The neuroprotective efficacy of K156 is comparable with commonly used obidoxime and oxime HI-6. Thus, none of the newly developed oximes achieves better neuroprotective efficacy than both commonly used oximes. They are therefore not suitable replacements for antidotal treatment of acute poisonings with cyclosarin.

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“…The rats were tested in a Functional Observational Battery (FOB) as described by Moser et al (1997). In brief, each rat was observed in a systematic fashion in the home cage, during handling and in an open field during a three minute observation period.…”

Section: Methodsmentioning

confidence: 99%

Toxicity Study of Di(2‐Ethylhexyl)phthalate (DEHP) in Combination with Acetone in Rats

Dalgaard¹,

Østergaard

Lam

et al. 2000

Pharmacology & Toxicology

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Abstract:In two separate studies with exposure duration 9 weeks or 4 weeks, male Wistar rats were dosed with di(2-ethylhexyl)phthalate (DEHP) by gavage and exposed to drinking water with or without acetone (0.5% wt/v in the 9-week study, 1.0% wt/v in the 4-week study). In the 9-week study the doses of DEHP were 0, 125, 250, 500 or 1000 mg/kg b.wt. In the 4-week study the doses of DEHP were increased to 1000, 5000 and 10,000 mg/kg b.wt. In the 9-week study, the relative liver weight was increased in the rats exposed to 500 and 1000 mg/kg b.wt. No interaction of DEHP and acetone was observed in any of the measured parameters. In the 4-week study DEHP, at the highest dose level, resulted in severe general toxicity. The group exposed to DEHP in combination with acetone was more affected. Male fertility was decreased. Body weight was decreased, and the relative weight of the liver, kidney, heart, brain and adrenals increased. The relative weight of the testes decreased in the 5000 and 10,000 mg/kg b.wt. groups. The weight of seminal vesicles and epididymals decreased at 10,000 mg/kg b.wt. In animals exposed to 5000 and 10,000 mg DEHP/kg b.wt. a severe atrophy of the seminiferous tubules and a slight diffuse Leydig's cell hyperplasia was observed. The cellular debris and conglomerates of desquamated cells found in the lumen of the seminiferous tubules were immunostained positive for vimentin. This indicates that Sertoli cell cytoplasm is included in the conglomerates -an interesting finding not previously described. No specific interaction of DEHP and acetone was observed in any of the measured parameters.It is well known that di(2-ethylhexyl)phthalate (DEHP) induces testicular atrophy in rats (Woodward 1988). Low doses of the monoester derivative mono(2-ethylhexyl)-phthalate (MEHP) have been shown to induce the same effect as the diester, and MEHP is considered the most potent metabolite of DEHP (Teirlynck et al. 1988). The role of other metabolites, e.g. 2-ethylhexanol is not fully elucidated. Both the phthalic acid and the aliphatic (ethylhexyl) part of DEHP may be toxic to the testis. The possible contribution of the aliphatic part to the testis toxicity can be examined by administering acetone concomitantly with DEHP. If enhancement of testis toxicity occurs, this may be interpreted as an indication of ethylhexyl contribution to toxicity. A small fraction of 2-ethylhexanol may be metabolised to a g-diketone, and it is well known that acetone potentiates the toxicity of g-diketones (Misumi & Nagano 1985). We have previously reported on the interaction between acetone and 2,5-hexanedione, the testis toxic metabolite of n-hexane (Larsen et al. 1991). The present study was performed to investigate a possible enhancement of the toxicity of DEHP by acetone in testis and the peripheral nervous system. Several Sertoli cell toxicants i.e. acrylamide, carbon disulfide, 2,5-hexanedione and tri-o-cresyl phosphate induce testis toxicity and peripheral neuropathy, possibly by affecting the cytoskeleton (Boekelheide 1993 the ne...

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The IPCS Collaborative Study on Neurobehavioral Screening Methods

Cited by 42 publications

References 18 publications

EthoVision: A versatile video tracking system for automation of behavioral experiments

EthoVision: A versatile video tracking system for automation of behavioral experiments

A Comparison of the Neuroprotective Efficacy of Newly Developed Oximes (K156, K203) and Currently Available Oximes (Obidoxime, HI-6) in Cyclosarin-poisoned Rats

Toxicity Study of Di(2‐Ethylhexyl)phthalate (DEHP) in Combination with Acetone in Rats

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