The ipdC, hisC1 and hisC2 genes involved in indole-3-acetic production used as alternative phylogenetic markers in Azospirillum brasilense

Jijón-Moreno, Saúl; Marcos-Jiménez, Cynthia; Pedraza, Raúl O.; Ramírez-Mata, Alberto; Salamone, Inés García de; Scavino, Ana Fernández; Vásquez-Hernández, Claudia A.; Soto-Urzúa, Lucía; Baca, Beatriz E.

doi:10.1007/s10482-015-0444-0

Cited by 20 publications

(12 citation statements)

References 35 publications

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“…The strains were cultured with shaking at 150 rpm and 30°C for 16, 24, 36, and 40 h. Auxin production was estimated by HPLC, using IAA (Sigma Chemical No. 87-51-4) as a standard as previously described [30]. The protein concentration was assessed by the Bradford method (Bradford reagent, Sigma-Aldrich No.…”

Section: Methodsmentioning

confidence: 99%

TyrR is involved in the transcriptional regulation of biofilm formation and D-alanine catabolism in Azospirillum brasilense Sp7.

et al. 2019

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Azospirillum brasilense is one of the most studied species of diverse agronomic plants worldwide. The benefits conferred to plants inoculated with Azospirillum have been primarily attributed to its capacity to fix atmospheric nitrogen and synthesize phytohormones, especially indole-3-acetic acid (IAA). The principal pathway for IAA synthesis involves the intermediate metabolite indole pyruvic acid. Successful colonization of plants by Azospirillum species is fundamental to the ability of these bacteria to promote the beneficial effects observed in plants. Biofilm formation is an essential step in this process and involves interactions with the host plant. In this study, the tyrR gene was cloned, and the translated product was observed to exhibit homology to TyrR protein, a NtrC/NifA-type activator. Structural studies of TyrR identified three putative domains, including a domain containing binding sites for aromatic amino acids in the N-terminus, a central AAA+ ATPase domain, and a helix-turn-helix DNA binding motif domain in the C-terminus, which binds DNA sequences in promoter-operator regions. In addition, a bioinformatic analysis of promoter sequences in A . brasilense Sp7 genome revealed that putative promoters encompass one to three TyrR boxes in genes predicted to be regulated by TyrR. To gain insight into the phenotypes regulated by TyrR, a tyrR -deficient strain derived from A . brasilense Sp7, named A . brasilense 2116 and a complemented 2116 strain harboring a plasmid carrying the tyrR gene were constructed. The observed phenotypes indicated that the putative transcriptional regulator TyrR is involved in biofilm production and is responsible for regulating the utilization of D-alanine as carbon source. In addition, TyrR was observed to be absolutely required for transcriptional regulation of the gene dadA encoding a D-amino acid dehydrogenase. The data suggested that TyrR may play a major role in the regulation of genes encoding a glucosyl transferase, essential signaling proteins, and amino acids transporters.

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Section: Methodsmentioning

confidence: 99%

TyrR is involved in the transcriptional regulation of biofilm formation and D-alanine catabolism in Azospirillum brasilense Sp7.

et al. 2019

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“…Jijón‐Moreno et al [125] studied other genes apart from ipd C involved in indole‐3‐pyruvate pathways. In Azospirillum strains, three genes, ipd C, his C1, and his C2, were present.…”

Section: Genetics Of Plant Growth Promotion By Endophytic Bacteriamentioning

confidence: 99%

Bacterial endophytes: Molecular interactions with their hosts

et al. 2021

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Plant growth promotion has been found associated with plants on the surface (epiphytic), inside (endophytic), or close to the plant roots (rhizospheric). Endophytic bacteria mainly have been researched for their beneficial activities in terms of nutrient availability, plant growth hormones, and control of soil‐borne and systemic pathogens. Molecular communications leading to these interactions between plants and endophytic bacteria are now being unrevealed using multidisciplinary approaches with advanced techniques such as metagenomics, metaproteomics, metatranscriptomics, metaproteogenomic, microRNAs, microarray, chips as well as the comparison of complete genome sequences. More than 400 genes in both the genomes of host plant and bacterial endophyte are up‐ or downregulated for the establishment of endophytism and plant growth‐promoting activity. The involvement of more than 20 genes for endophytism, about 50 genes for direct plant growth promotion, about 25 genes for biocontrol activity, and about 10 genes for mitigation of different stresses has been identified in various bacterial endophytes. This review summarizes the progress that has been made in recent years by these modern techniques and approaches.

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“…The complete biosynthetic pathway of indole-3-carboxylic acid is not studied so far, but it was reported that L-phenylalanine is converted in three steps to indole-3acetic acid, which is catalyzed by different enzymes to indole-3carboxylic acid. The biosynthesis of indole-3-acetic acid has been well studied in various plant-associated bacteria [34]. The first step of this pathway is the transfer of the amino group from L-tryptophan to 2oxoglutarate by an aminotransferase, forming indole-3-pyruvate and glutamate.…”

Section: Biosynthesis Of Indole-3-carboxylic Acidmentioning

confidence: 99%

Insight into Saccharothrix espanaensis Grown in Different Media:Transcriptome and Natural Product Analysis

Samra¹,

Tokovenko²,

Kaszubowska³

et al. 2018

Nat Prod Chem Res

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In this study, we show that cultivating of Saccharothrix espanaensis in the amino acid-rich SPY medium led to the detection of antibacterial and antifungal products. The cultivation in GYM-, HA-or NL111-medium did not lead to the production of these compounds. Four products were isolated from Saccharothrix espanaensis-SPY culture and determined as the known compounds anthranilic acid, indol-3-carboxylic acid, benzoic acid, and phenylacetic acid. In order to investigate the influence of SPY medium on gene transcription and to identify candidate genes involved in the biosynthesis of the purified compounds a comparative RNA sequencing analysis of Saccharothrix espanaensis cultivated in different media was performed. Total transcriptome analysis demonstrated that media composition has an influence on the expression of several genes. Among them are genes encoding enzymes belong to amino acid metabolism enzymes. Furthermore, based on the collected data genes involved in the biosynthesis of anthranilic acid, indol-3-carboxylic acid, benzoic acid, and phenylacetic acid could be predicted. Deletion of the genes BN6_01860 and BN6_67950 revealed their importance in the biosynthesis of indole-3carboxylic acid and benzoic acid, respectively.

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The ipdC, hisC1 and hisC2 genes involved in indole-3-acetic production used as alternative phylogenetic markers in Azospirillum brasilense

Cited by 20 publications

References 35 publications

TyrR is involved in the transcriptional regulation of biofilm formation and D-alanine catabolism in Azospirillum brasilense Sp7.

TyrR is involved in the transcriptional regulation of biofilm formation and D-alanine catabolism in Azospirillum brasilense Sp7.

Bacterial endophytes: Molecular interactions with their hosts

Insight into Saccharothrix espanaensis Grown in Different Media:Transcriptome and Natural Product Analysis

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