The iron uptake repressor Fep1 in the fission yeast binds Fe-S cluster through conserved cysteines

Kim, Hyojin; Lee, Kang-Lok; Kim, Kyoung-Dong; Roe, Jung‐Hye

doi:10.1016/j.bbrc.2016.07.070

Cited by 24 publications

(9 citation statements)

References 31 publications

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“…The HMG_box (high mobility group box) in S. cerevisiae is seen in three proteins: Spp41, which is involved in the negative expression regulation of spliceosome components [ 133 ]; Nhp6a, which is required for the fidelity of some tRNA genes [ 134 ]; and Ixr1, a transcriptional repressor that regulates hypoxic genes [ 135 ]. Fep1, an example of Znf_GATA, was reported to be a transcriptional repressor involved in the regulation of some iron transporter genes under high iron concentrations [ 136 ]. S. cerevisiae Mbf1, belonging to Cro/C1-type HTH, is a transcriptional coactivator [ 137 ].…”

Section: Resultsmentioning

confidence: 99%

Conservation and Expansion of Transcriptional Factor Repertoire in the Fusarium oxysporum Species Complex

Haridas

et al. 2023

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The Fusarium oxysporum species complex (FOSC) includes both plant and human pathogens that cause devastating plant vascular wilt diseases and threaten public health. Each F. oxysporum genome comprises core chromosomes (CCs) for housekeeping functions and accessory chromosomes (ACs) that contribute to host-specific adaptation. This study inspects global transcription factor profiles (TFomes) and their potential roles in coordinating CC and AC functions to accomplish host-specific interactions. Remarkably, we found a clear positive correlation between the sizes of TFomes and the proteomes of an organism. With the acquisition of ACs, the FOSC TFomes were larger than the other fungal genomes included in this study. Among a total of 48 classified TF families, 14 families involved in transcription/translation regulations and cell cycle controls were highly conserved. Among the 30 FOSC expanded families, Zn2-C6 and Znf_C2H2 were most significantly expanded to 671 and 167 genes per family including well-characterized homologs of Ftf1 (Zn2-C6) and PacC (Znf_C2H2) that are involved in host-specific interactions. Manual curation of characterized TFs increased the TFome repertoires by 3% including a disordered protein Ren1. RNA-Seq revealed a steady pattern of expression for conserved TF families and specific activation for AC TFs. Functional characterization of these TFs could enhance our understanding of transcriptional regulation involved in FOSC cross-kingdom interactions, disentangle species-specific adaptation, and identify targets to combat diverse diseases caused by this group of fungal pathogens.

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Section: Resultsmentioning

confidence: 99%

Conservation and Expansion of Transcriptional Factor Repertoire in the Fusarium oxysporum Species Complex

Haridas

et al. 2023

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“…pombe it has been suggested that GrxD signals iron starvation to Fep1 by removing iron, not [2Fe-2S], bound by Fep1 [46]. Later on, it was shown that Fep1 coordinates a [2Fe-2S] cluster, not iron, by a highly conserved CRR [57]. Nevertheless, GrxD-mediated removal of [2Fe-2S] clusters bound by SreA and HapX appears to be a conceivable mode of action for signaling iron starvation.…”

Section: Discussionmentioning

confidence: 99%

The monothiol glutaredoxin GrxD is essential for sensing iron starvation in Aspergillus fumigatus

et al. 2019

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Efficient adaptation to iron starvation is an essential virulence determinant of the most common human mold pathogen, Aspergillus fumigatus. Here, we demonstrate that the cytosolic monothiol glutaredoxin GrxD plays an essential role in iron sensing in this fungus. Our studies revealed that (i) GrxD is essential for growth; (ii) expression of the encoding gene, grxD, is repressed by the transcription factor SreA in iron replete conditions and upregulated during iron starvation; (iii) during iron starvation but not iron sufficiency, GrxD displays predominant nuclear localization; (iv) downregulation of grxD expression results in de-repression of genes involved in iron-dependent pathways and repression of genes involved in iron acquisition during iron starvation, but did not significantly affect these genes during iron sufficiency; (v) GrxD displays protein-protein interaction with components of the cytosolic iron-sulfur cluster biosynthetic machinery, indicating a role in this process, and with the transcription factors SreA and HapX, which mediate iron regulation of iron acquisition and iron-dependent pathways; (vi) UV-Vis spectra of recombinant HapX or the complex of HapX and GrxD indicate coordination of iron-sulfur clusters; (vii) the cysteine required for iron-sulfur cluster coordination in GrxD is in vitro dispensable for interaction with HapX; and (viii) there is a GrxD-independent mechanism for sensing iron sufficiency by HapX; (ix) inactivation of SreA suppresses the lethal effect caused by GrxD inactivation. Taken together, this study demonstrates that GrxD is crucial for iron homeostasis in A. fumigatus.

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“…SreA homologs possess four conserved cysteine residues in between the GATA-type zinc fingers 94. The SreA homolog of S. pombe , Fep1, has been demonstrated to coordinate iron or FeS depending on the presence of these conserved cysteine residues when expressed recombinantly in Escherichia coli , and, moreover, these cysteine residues were shown to be essential for Fep1 function 13,40. Similarly, the recombinant SreA homolog from Pichia pastoris was shown to coordinate a [2Fe–2S] cluster with the four conserved cysteines,100 while the recombinant SreA homolog of Neurospora crassa exhibited a reddish color depending on the presence of the conserved four cysteine residues 101.…”

Section: Discussionmentioning

confidence: 99%

Iron-sensing is governed by mitochondrial, not by cytosolic iron–sulfur cluster biogenesis inAspergillus fumigatus

et al. 2018

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The iron uptake repressor Fep1 in the fission yeast binds Fe-S cluster through conserved cysteines

Cited by 24 publications

References 31 publications

Conservation and Expansion of Transcriptional Factor Repertoire in the Fusarium oxysporum Species Complex

Conservation and Expansion of Transcriptional Factor Repertoire in the Fusarium oxysporum Species Complex

The monothiol glutaredoxin GrxD is essential for sensing iron starvation in Aspergillus fumigatus

Iron-sensing is governed by mitochondrial, not by cytosolic iron–sulfur cluster biogenesis inAspergillus fumigatus

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