The Isolated Perfused Bovine Udder as a Model of Dermal Eicosanoid Release

Abstract

The aim of this study was to examine whether the isolated perfused bovine udder could be used as a suitable in vitro inflammation model. A common in vivo inflammation model is arachidonic acid-induced inflammation in mouse ears. As an in vitro substitute for this model, arachidonic acid was administered topically to the skin of an isolated perfused bovine udder and the subsequent changes in eicosanoid synthesis were examined. As with the mouse-ear model, there was a significant increase in eicosanoid synthesis (prostaglandins E2 and F2α and leukotrienes B4 and C4/D4/E4) following topical irritation. This effect lasted for 3 hours. In addition, the changes in prostaglandin E2 synthesis in the skin following irritation with arachidonic acid were measured by the microdialysis technique. In conclusion, the in vitro model described seems suitable for studies of pharmacological effects on eicosanoid synthesis.

Effects of steroidal and non-steroidal antiphlogistic drugs on eicosanoid synthesis in irritated skin: studies with the isolated perfused bovine udder

“…Therefore, we have established a com-parable in-vitro model of arachidonic acid-induced skin inflammation. Former studies demonstrated the increase of eicosanoids in arachidonic acid-irritated skin of isolated perfused bovine udders (Ba$ umer & Kietzmann 2000). In this study we have examined the effect of topically and '' systemically '' administered antiphlogistic drugs on the eicosanoid synthesis in the irritated bovine udder skin.…”

“…Bovine udders were perfused according to former studies (Kietzmann et al 1993 ;Ba$ umer & Kietzmann 2000). In brief, the organs were collected immediately after the slaughter of healthy cows.…”

“…In similarity to the established mouse ear model (Opas et al 1985 ;Rao et al 1993 ;Lloret & Moreno 1995), arachidonic acid was administered topically onto the udder skin. As parameters, the dermal concentration of eicosanoids, which play a pivotal role in inflammatory processes, was measured in the skin (Ba$ umer & Kietzmann 2000).…”

Microdialysis of Inflammatory Mediators in the Skin: A Review

“…for instance, superficial implantation of microdialysis probes in an isolated perfused bovine udder model has been used to study experimental inflammation. Topical application of arachidonic acid led to the release of a number of eicosanoids of which prostaglandin E2 (PgE2) was measured by microdialysis (22). The bovine udder model was also used to study inflammatory reactions to biodegradable titanium implants (23).…”

Prostaglandin E2 as a read out for endotoxin detection in a bovine whole blood assay

“…Moreover, PGE 2 is not species-specific and assay kits for the detection of this eicosanoid are available commercially and have already been used in the bovine system (B€ aumer & Kietzmann, 2000(B€ aumer & Kietzmann, , 2001B€ aumer et al, 2002;Braun & Kietzmann, 2004). Prostaglandin E 2 was also used as read out for the human MAT (Hartung & Wendel, 1995) and in a bovine whole blood assay (Usui et al, 2013).…”