The isolated perfused porcine skin flap (IPPSF) I. A novel in vitro model for percutaneous absorption and cutaneous toxicology studies

Riviere, Jim E.; Bowman, Karl F.; Monteiro‐Riviere, Nancy A.; Dix, Lynn P.; Carver, Michael P.

doi:10.1016/0272-0590(86)90094-1

Cited by 150 publications

(50 citation statements)

References 12 publications

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“…Island skin flaps [4][5][6] are often used in rats to study ischemic phenom ena and associated cutaneous metabolism modifications in order to find protective agents. Other models have been proposed for studying percutaneous drug absorption and its influence upon their passage into the blood: these include the isolated perfused porcine skin flap [7], the rat/human skin flap system [8] and recently the rat sandwich skin flap [9,10].…”

Section: Introductionmentioning

confidence: 99%

A New Model: Bipediculated Dorsal Flap of Hairless Rat for Cutaneous Blood Flow Evaluation

Auclair

Besnard²,

Dupont³

et al. 1989

Skin Pharmacol Physiol

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To study the influence of cutaneous blood flow upon the transdermal penetration of drugs, an original in vivo model, the bipediculated dorsal flap (BDF) of the hairless rat is described. This model is constituted by a dorsal cutaneous muff on both sides of the spinal column. The blood circulation was maintained symmetrically on both skin insertion pedicles. Two radiotracers, ⁸⁶Rb and ²⁰¹T1 (respective half-lives: 18 and 3 days), known to measure nutritional blood flows, are compared in this model. In the back skin, over and under the flap, mean blood flow (expressed as percentage of injected dose per gram skin) was similar with Rb (9.5 ± 3.1) ·10^-2 and Tl (10.2 ± 2.1)· 10^-2. Equivalent values were obtained in BDF skin (non ligated pedicle): Rb (8.4 ± 1.3) · 10^-2, Tl (9.5 ± 2.7) · 10^-2. In BDF skin, cutaneous blood flow was lower by 70–91 % in the ligated pedicle (compared to the nonligated) for both radioisotopes. Identical ⁸⁶Rb and ²⁰¹T1 distributions suggest the use of ²⁰¹T1 for further experiments of cutaneous blood flow.

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Section: Introductionmentioning

confidence: 99%

A New Model: Bipediculated Dorsal Flap of Hairless Rat for Cutaneous Blood Flow Evaluation

Auclair

Besnard²,

Dupont³

et al. 1989

Skin Pharmacol Physiol

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“…The isolated perfused porcine skin flap was developed to overcome some of the shortcomings of the classic in vitro methods. It provided an anatomically intact, viable, isolated, perfused tube-like preparation [58,59]. The pig was selected because of its size and because its skin was found to be functionally and structurally similar to that of man.…”

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confidence: 99%

The Pig as an Experimental Animal Model of Percutaneous Permeation in Man: Qualitative and Quantitative Observations – An Overview

Simon¹,

Maibach²

2000

Skin Pharmacol Physiol

342

202

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The pig has been a well-recognized experimental animal in biomedical research for many centuries. Physiological and anatomical similarities between man and pig made this animal a good model for man in many research areas. Pharmacological and toxicological research on the skin is often based on knowledge of skin absorption and percutaneous permeation. Anatomical, physiological and biochemical similarities are cited and various uses of the pig as a model for man in the investigation of skin permeation are reviewed. Further, several isolated organ models are reviewed. The importance of full details of the experimental animal, namely its age, sex, breed, size (weight) and body region, is emphasized.

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“…This method is a self-contained system that mimics skin perfusion by maintaining vascular supply to the tissues using an artificial buffer solution. Further details of the method can be found in (Riviere et al, 1986). The study shows that aliphatic marker compounds are absorbed slower than Naphthalene.…”

Section: Key In Vitro Dermal Bioavailability Studiesmentioning

confidence: 99%

A review of the current state of the art of physiologically-based tests for measuring human dermal in vitro bioavailability of polycyclic aromatic hydrocarbons (PAH) in soil

Beriro

Cave

Wragg

et al. 2016

Journal of Hazardous Materials

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Polycyclic Aromatic Hydrocarbons are classed as Persistent Organic Pollutants, a large group of compounds that share similar characteristics. They are lipophilic, resistant to degradation in the environment and harmful to human and environmental health. Soil has been identified as the primary reservoir for Polycyclic Aromatic Hydrocarbons in the United Kingdom. This study reviews the literature associated with, or is relevant to, the measurement and modelling of dermal absorption of Polycyclic Aromatic Hydrocarbons from soils. The literature illustrates the use of in vivo, in vitro and in silico methods from a wide variety of scientific disciplines including occupational and environmental exposure, medical, pharmaceutical and cosmetic research and associated mathematical modelling. The review identifies a number of practical shortcomings which must be addressed if they are to be applied to high throughput laboratory analysis of contaminated soils for human health risk assessment.

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The isolated perfused porcine skin flap (IPPSF) I. A novel in vitro model for percutaneous absorption and cutaneous toxicology studies

Cited by 150 publications

References 12 publications

A New Model: Bipediculated Dorsal Flap of Hairless Rat for Cutaneous Blood Flow Evaluation

A New Model: Bipediculated Dorsal Flap of Hairless Rat for Cutaneous Blood Flow Evaluation

The Pig as an Experimental Animal Model of Percutaneous Permeation in Man: Qualitative and Quantitative Observations – An Overview

A review of the current state of the art of physiologically-based tests for measuring human dermal in vitro bioavailability of polycyclic aromatic hydrocarbons (PAH) in soil

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