The Isolation and Activity of Growth-Stimulating Factors from Human Platelets

Abstract

Optimal conditions for determining the mitogenic activity of platelet growth factor in a homologous System (human palmar fascia fibroblasts/human platelet growth factor) were established. With the aid of this System it was possible to test the active fraction from crude platelet extract following affinity chromatography on heparin-Sepharose, chromatofocusing and molecular sieve filtration. This fraction has an isoelectric point of pH >9 with a molecular mass of about 12000 daltons and reacts with ß-thromboglobulin antiserum.