The isolation and characterization of rat sublingual mucus-glycoprotein

Moschera, John; Pigman, Ward

doi:10.1016/s0008-6215(00)82668-3

Cited by 112 publications

(66 citation statements)

References 44 publications

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“…The major mucins of adult sublingual glands of rats and mice have been isolated and partially characterized. Based on size, amino acid and carbohydrate composition, and antigenicity, investigators have concluded that the sublingual gland mucin is very different from the mucin found in the seromucous acinar cells of the adult rodent submandibular gland (Moschera and Pigman, 1975;Roukema et al, 1976;Denny and Denny, 1984;Tabak et al, 1985;Culp et al, 1991;Man et al, 1995). Recent comparison of cDNA sequences of mucin transcripts from mouse submandibular and sublingual glands revealed very little homology between the two and confirmed their separate genetic origins (Denny and Denny, unpublished).…”

Section: (2) Perinatal and Adult Protein Phenotypesmentioning

confidence: 99%

Salivary Glands: A Paradigm for Diversity of Gland Development

Denny

Ball

Redman

1997

Critical Reviews in Oral Biology & Medicine

179

171

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The major salivary glands of mammals are represented by three pairs of organs that cooperate functionally to produce saliva for the oral cavity. While each type of gland produces a signature secretion that complements the secretions from the other glands, there is also redundancy as evidenced by secretion of functionally similar and, in some cases, identical products in the three glands. This, along with their common late initiation of development, in fetal terms, their similarities in developmental pattern, and their proximate sites of origin, suggests that a common regulatory cascade may have been shared until shortly before the onset of overt gland development. Furthermore, occasional ectopic differentiation of individual mature secretory cells in the wrong" gland suggests that control mechanisms responsible for the distinctive cellular composition of each gland also share many common steps, with only minor differences providing the impetus for diversification. To begin to address this area, we examine here the origins of the salivary glands by reviewing the expression patterns of several genes with known morphogenetic potential that may be involved based on developmental timing and location. The possibility that factors leading to determination of the sites of mammalian salivary gland development might be homologous to the regulatory cascade leading to salivarv gland formation in Drosophila is also evaluated. In a subsequent section, cellular phenotypes of neonatal and adult glands are compared and evaluated for insights into the mechanisms and lineages leading to cellular diversification. Finally, the phenomena of proliferation, repair, and regeneration in adult salivary glands are reviewed, with emphasis on the extent to which the cellular diversity is reversible and which cell type other than stem cells has the ability to redifferentiate into other cell types.

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Section: (2) Perinatal and Adult Protein Phenotypesmentioning

confidence: 99%

Salivary Glands: A Paradigm for Diversity of Gland Development

Denny

Ball

Redman

1997

Critical Reviews in Oral Biology & Medicine

179

171

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“…Despite these differences in compartmental volume, the absolute number of cells did not differ between groups for any of the epithelial compartments, except for the excretory ducts where the number of cells was 193% larger in males compared to females. However, the mean cell volume of mucous acinar cells and striated and excretory duct cells was significantly higher in male rats (13,33, and 47%, respectively), while the mean cell volume of serous demilune cells was 38% higher in females. A significant difference in the total external surface of the various compartments was only observed for the excretory ducts, which was 74% greater in males.…”

Section: Resultsmentioning

confidence: 82%

“…The mucous acinar cells produce and secrete sublingual mucins or mucous glycoproteins 13,28 , while the serous demilune cells secrete common salivary protein 1 (CSP-1), and neonatal submandibular gland secretory proteins B (SMGB) and D (SMGD) 2,3,7,12 . The innervation of these cells is mainly parasympathetic 27 and they rapidly respond to stimulation with secretagogues 6 .…”

Section: Discussionmentioning

confidence: 99%

Morphometric characterization of sexual differences in the rat sublingual gland

et al. 2004

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ABSTRACT:The presence of morphological differences in the sublingual gland of male and female adult rats was determined by morphometry. Absolute and relative glandular mass was 21% lower and 31% higher, respectively, in females than in males. The fractions of glandular volume occupied by the mixed acini, intercalated ducts and striated ducts did not differ significantly between genders; however, their absolute volume was respectively 29, 42 and 58% higher in males. Despite the differences in the volume of these morphological compartments, the number of cells did not differ significantly between genders, except for the excretory duct compartment, for which a larger number was observed in males. With respect to cell volume, 13, 33 and 47% higher volumes were observed in males for mucous acinar cells and striated and excretory duct cells, respectively, while a 38% higher volume of serous demilune cells was observed for females. The surface-to-volume ratio of acini and striated ducts was respectively 16 and 35% higher in females. Based on these results, we conclude that the sublingual gland of female rats possesses smaller acini, and shorter ducts whose caliber is narrower, smaller mucous acinar and larger serous cells than the ones found in the male gland, indicating the presence of sexual dimorphism as well as suggesting sexual differences in the quality of the secreted product. DESCRIPTORS: Sublingual gland; Rats; Sex characteristics. RESUMO:A ocorrência de diferenças morfológicas entre sexos na glândula sublingual de ratos adultos foi verificada pela morfometria. As massas glandular absoluta e relativa das fêmeas foi, respectivamente, 21% menor e 31% maior que as dos machos. As frações de volume glandular ocupadas pelos ácinos mistos, ductos intercalares e ductos estriados não mostraram diferenças significantes entre sexos, no entanto, os seus volumes absolutos foram, respectivamente, 29%, 42% e 58% maiores nos machos. Apesar dessas diferenças nos volumes compartimentais, os seus conteúdos em número de células não apresentaram diferenças significantes entre sexos, exceto o compartimento dos ductos excretores, que mostrou maior número nos machos. Quanto ao volume celular, as células acinosas mucosas, as dos ductos estriados e as dos ductos excretores mostraram volumes, respectivamente, 13%, 33% e 47% maiores nos machos, e o das células das semiluas serosas foi 38% maior nas fêmeas. A relação superfície-volume dos ácinos e dos ductos estriados foi, respectivamente, 16% e 35% maior nas fêmeas. Baseados nos resultados obtidos, concluímos que as glândulas sublinguais das fêmeas exibem ácinos menores e ductos mais curtos e menos calibrosos e células acinosas mucosas menores e serosas maiores do que nos machos, indicando a ocorrência de dimorfismo sexual, e sugerindo que possa haver também diferenças na qualidade do produto secretado. DESCRITORES: Glândula sublingual; Ratos; Características sexuais. HistologyLima MC, Sottovia-Filho D, Cestari TM, Taga R. Morphometric characterization of sexual differences in the rat su...

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“…Sialic acid containing acetylic groups on C 4 of pyranose ring [14] was detected at the epithelial cells of all the fertile women, but only of some postmenopausal women. Sialic acid with C 7 and/or C 8 and/or C 9 -Oacetylic groups in the side chain was observed in the epithelial cells of all the patients both in the fertile and postmenopausal women [15].…”

Section: Discussionmentioning

confidence: 97%

“…Deacetylation was performed incubating the sections with 0.1% KOH in 70% ethanol for 30 min at room temperature. This treatment, detaching all the acetyl substituents, renders sialic acid residues, which contain also acetylic groups on C 4 of the pyranose ring, susceptible to sialidase digestion [14].…”

Section: Enzymatic and Chemical Treatmentsmentioning

confidence: 99%

The sialoglycoconjugates in the oviducts of fertile and postmenopausal women

Gheri¹,

Noci

Gheri

et al. 2007

Maturitas

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Objectives: The aim of the study was to investigate the distribution of the sialoderivatives of the glycoconjugates in the oviduct of normally menstruating and postmenopausal women. Methods: Biopsies of ampullary and isthmic portions of the oviduct were obtained from fertile women, in proliferative and secretive phases, and from postmenopausal subjects. The study was carried out using digoxigenin-labelled lectins (MAA, SNA and PNA) in addition to the use of enzymatic and chemical treatments to characterize the different glycosidic linkages of the sialoderivatives and to obtain information on their structure. Results: No appreciable difference in sialoderivatives distribution was observed among the oviducts, particularly at the epithelium luminal surface, of the fertile women in the two menstrual cycle phases or among those of the fertile and some postmenopausal women, independently from age. Moreover, no appreciable difference of distribution was observed between the ampullary and the isthmic portions in both the study groups. Conclusions: In the fertile women sialoderivatives present at the luminal surface of the epithelial cells could play a role in sperm capacitation and mobility, and facilitate the transit of the egg and of the early embryo along the oviducts. The similar distribution of sialoderivatives in the oviduct epithelium of some postmenopausal women of different age, compared to the fertile ones, suggests a maintaining of some functions of the organ, independently from the age of the woman. This could be due, in some cases, to the influence of remaining still sufficient sex hormonal levels.

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The isolation and characterization of rat sublingual mucus-glycoprotein

Cited by 112 publications

References 44 publications

Salivary Glands: A Paradigm for Diversity of Gland Development

Salivary Glands: A Paradigm for Diversity of Gland Development

Morphometric characterization of sexual differences in the rat sublingual gland

The sialoglycoconjugates in the oviducts of fertile and postmenopausal women

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