The isolation and characterization of putative mesenchymal stem cells from the spiny mouse

Abstract

The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSCs may be isolated from different species the aim of this study was to isolate and characterize putative bone marrow derived MSCs from the spiny mouse, Acomys cahirinus. MSCs were isolated from the spiny mouse in a traditional manner, and based on plastic adherence, morphology, colony forming unitfibroblast assays and functional assessment (adipogenic, osteogenic and chondrogenic differentiation potential) a population of putative mesenchymal stem cells from the compact bone of the spiny mouse have been isolated and characterized. Such methodological approaches overcome the lack of species-specific antibodies for the spiny mouse and could be employed for other species where the cost of generating species-specific antibodies is not warranted.

Characterization and Classification of Mesenchymal Stem Cells in Several Species Using Surface Markers for Cell Therapy Purposes

“…Also, isolated MSCs from three different sources (bone marrow, synovial membrane and adipose tissue) expressed CD44 and MHC-I [17], while using mouse antibodies, no expression of CD73, CD90 and CD105 was seen in Ovine endometrial MSCs [18]. In a study by Grzesiak et al [19] in which human antibodies were used, ovine adipose derived MSCs showed low expression of CD90 and CD105; isolated bone-marrow and adipose tissue MSCs from Balb/c, C3H and C5BL/6 mice were positive for CD29, CD44, CD105 and Sca-1, but negative for CD34, TER-119, CD45, and CD11b [20]; mouse BM-MSCs were positive for CD44, CD29, CD31, CD44, CD41, CD105 and SCA-1,and negative for CD45, CD3e, CD34, CD45 and CD117 [21]. Additionally, immunophenotypic analysis demonstrated that murine epiphysis derived mesenchymal stem cells were positive for CD29, CD44, CD73, CD105, CD166, Sca-1 and SSEA-4, and negative for CD31, CD34, CD11b and CD45 [22].…”

Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models

“…Stem cell identification through the genotypic approach using specific primers is known as the polymerase chain reaction approach. In preparing the stem stage, the expression of negative CD45 and positive CD73, CD90, and CD105 was characterized to ensure that the stem cells inserted were mesenchymal [14][15][16][17][18].…”

Aucubin Promotes Osteogenic Differentiation and Facilitates Bone Formation through the lncRNA‐H19 Driven Wnt/β‐Catenin Signaling Regulatory Axis

“…Fresh bone marrow-derived mesenchymal stem cells (BM-MSCs) were isolated from 4-week-old C57BL/6 mice according to previous publication [ 24 ]. Briefly, bone marrow was flushed out with a syringe and needle, and cultured in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% FBS and 1% penicillin/streptomycin.…”