The Isolation and Determination of Sulforaphane from Broccoli Tissues by Reverse Phase‐High Performance Liquid Chromatography

Azizi, Seyed Naser; Amiri-Besheli, Behnam; Sharifi-Mehr, Salman

doi:10.1002/jccs.201190143

Cited by 17 publications

(3 citation statements)

References 23 publications

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“…The aglycon organosulfides identified here have previously been reported from broccoli florets, , although most interest has been in sulforaphane and sulforaphane nitrile because of the putative anticancer properties of the former , and possibly because it is the main aglycon from broccoli . Comparisons between the amounts of particular aglycons, such as sulforaphane, have been conducted from different parts of broccoli plants, , but we have not been able to find reports that discuss the range of compounds from the different tissues. We recently identified the MeSe aglycons from broccoli florets, these tissues being of interest because they are the most consumed parts of these plants.…”

Section: Resultsmentioning

confidence: 82%

Distribution of Selenoglucosinolates and Their Metabolites in Brassica Treated with Sodium Selenate

Matich

McKenzie

Lill

et al. 2015

J. Agric. Food Chem.

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In Brassica species, hydrolysis of (methylthio)glucosinolates produces sulfur-containing aglycons which have demonstrated anticancer benefits. Selenized Brassicaceae contain (methylseleno)glucosinolates and their selenium-containing aglycons. As a prelude to biological testing, broccoli, cauliflower, and forage rape plants were treated with sodium selenate and their tap roots, stems, leaves, and florets analyzed for selenoglucosinolates and their Se aglycons. Two new selenoglucosinolates were identified: glucoselenoraphanin in broccoli florets and glucoselenonasturtiin in forage rape roots. A new aglycon, selenoberteroin nitrile, was identified in forage rape. The major selenoglucosinolates were glucoselenoerucin in broccoli, glucoselenoiberverin in cauliflower, and glucoselenoerucin and glucoselenoberteroin in forage rape roots. In broccoli florets, the concentrations of selenglucosinolates exceeded those of their sulfur analogues. Fertilization with selenium slightly reduced (methylthio)glucosinolates and aglycons in the roots, but increased them in the florets, the leaves, and sometimes the stems. These discoveries provide a new avenue for investigating how consumption of Brassica vegetables and their organoselenides may promote human health.

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Section: Resultsmentioning

confidence: 82%

Distribution of Selenoglucosinolates and Their Metabolites in Brassica Treated with Sodium Selenate

Matich

McKenzie

Lill

et al. 2015

J. Agric. Food Chem.

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“…Chemical synthesis of SFN is costly and time-consuming, requires several highly toxic substances, and the final products from these reactions require further purification. These disadvantages limit the use of synthesized SFN as a food additive [20] and necessitate the development of natural SFNs.…”

Section: Anti-adipogenic Effects Of Sulforaphane-rich Ingredient With...mentioning

confidence: 99%

Anti-adipogenic Effects of Sulforaphane-rich Ingredient with Broccoli Sprout and Mustard Seed in 3T3-L1 Preadipocytes

et al. 2022

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Glucoraphanin (GRA) is a precursor of sulforaphane (SFN), which can be synthesized by the enzyme myrosinase. In this study, we developed and validated HPLC analytical methods for the determination of GRA and SFN in mustard seed powder (MSP), broccoli sprout powder (BSP), and the MSP-BSP mixture powder (MBP) and evaluated their anti- adipogenic effects in 3T3-L1 adipocytes. We found that the analysis methods were suitable for the determination of GRA and SFN in MSP, BSP, and MBP. The content of GRA in BSP was 131.11±1.84 μmol/g, and the content of SFN in MBP was 162.29±1.24 μmol/g. In addition, BSP and MBP effectively decreased lipid accumulation content without any cytotoxicity. Both BSP and MBP can significantly inhibited the expression of adipogenic proteins and increased the expression of proteins related to lipolysis and lipid metabolism. BSP and MBP inhibited the expression of adipocyte protein 2 (aP2), CCAAT/enhancer-binding protein-α (C/EBP-α), and peroxisome proliferator-activated receptor-γ (PPAR-γ) in 3T3-L1 adipocytes and inhibited the expression of fatty acid synthase (FAS) through AMP-activated protein kinase (AMPK). Meanwhile, BSP and MBP also increased the expression of the lipolysis-related proteins uncoupling protein-1 (UCP-1) and carnitine palmitoyltransferase-1 (CPT-1). Moreover, MBP exert anti-adipogenic to a greater extent than BSP in 3T3-L1 preadipocytes.

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“…The determination of ITCs with GC-MS has been employed [104][105][106][107][154][155][156]. For the analysis of volatile ITCs, the eco-friendly technique of Solid Phase Microextraction (SPME) in conjunction with GC-MS has been also proposed [157][158][159][160][161].…”

Section: Gas Chromatography/mass Spectrometrymentioning

confidence: 99%

Current Methods for the Extraction and Analysis of Isothiocyanates and Indoles in Cruciferous Vegetables

et al. 2021

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Cruciferous vegetables are characterized by the presence of sulfur-containing secondary plant metabolites known as glucosinolates (GLS). The consumption of cruciferous vegetables such as broccoli, cabbage, rocket salad, and cauliflower has been related to the prevention of non-communicable diseases. Their beneficial effects are attributed to the enzymatic degradation products of GLS, e.g., isothiocyanates and indoles. Owing to these properties, there has been a shift in the last few years towards the research of these compounds and a wide range of methods for their extraction and analytical determination have been developed. The aim of this review is to present the sample preparation and extraction procedures of isothiocyanates and indoles from cruciferous vegetables and the analytical methods for their determination. The majority of the references that have been reviewed are from the last decade. Although efforts towards the application of eco-friendly non-conventional extraction methods have been made, the use of conventional solvent extraction is mainly applied. The major analytical techniques employed for the qualitative and quantitative analysis of isothiocyanates and indoles are high-performance liquid chromatography and gas chromatography coupled with or without mass spectrometry detection. Nevertheless, the analytical determination of isothiocyanates presents several problems due to their instability and the absence of chromophores, making the simultaneous determination of isothiocyanates and indoles a challenging task.

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The Isolation and Determination of Sulforaphane from Broccoli Tissues by Reverse Phase‐High Performance Liquid Chromatography

Cited by 17 publications

References 23 publications

Distribution of Selenoglucosinolates and Their Metabolites in Brassica Treated with Sodium Selenate

Distribution of Selenoglucosinolates and Their Metabolites in Brassica Treated with Sodium Selenate

Anti-adipogenic Effects of Sulforaphane-rich Ingredient with Broccoli Sprout and Mustard Seed in 3T3-L1 Preadipocytes

Current Methods for the Extraction and Analysis of Isothiocyanates and Indoles in Cruciferous Vegetables

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