The Isolation and Performance Studies of an Alginate Degrading and Ethanol Producing Strain

Zhang, W.; Zhang, J.; Cui, Hongxia

doi:10.15255/cabeq.2013.1888

Cited by 20 publications

(9 citation statements)

References 23 publications

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“…In the fermentation process, cells grow first, and then release extracellular enzyme to realize the utilization of alginate. Therefore, the soluble sugar generated by pretreatment is beneficial to the cell growth and can significantly improve the yield of ethanol [ 27 ]. This point can also be confirmed by the measurement of soluble sugar.…”

Section: Resultsmentioning

confidence: 99%

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Ethanol Production from Colpomenia sinuosa by an Alginate Fermentation Strain Meyerozyma guilliermondii

Mao

Liu

Wang³

2021

Indian J Microbiol

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With the consumption of energy and the spread of COVID-19, the demand for ethanol production is increasing in the world. The industrial ethanol fermentation microbes cannot metabolize the alginate component of macro algae, which affects the ethanol yield. In this research, the ethanol production process from macro algae by an alginate fermentation yeast Meyerozyma guilliermondii , especially the pretreatment process of Colpomenia sinuosa, was studied. At the same time, the experimental design of Box-Behnken was carried out to achieve the optimum fermentation performance. The concentration of KH 2 PO 4 (A: 2–6 g.L −1 ), pH (B: 4–7), reaction time (C: 60–120 h) and temperature (D: 24–34 °C) were variable input parameters. During the ethanol production process, the algae powder was firstly mixed with water at 90 °C for 0.5 h. Later the fermentation culture medium was prepared and then it was fermented by the yeast Meyerozyma guilliermondii to produce ethanol. And the optimal fermentation parameters were as follows: fermentation temperature of 28 °C, KH 2 PO 4 dosage of 4.7 g.L −1 , initial pH of 6, and fermentation time of 99 h. The ethanol yield reached 0.268 g.g −1 (ethanol to algae), close to the predicted value of model. The generation of alginate lyase during the fermentation of algae was also examined. The highest alginate lyase activity reached 46.42 U.mL −1 .

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Section: Resultsmentioning

confidence: 99%

“…The pH value is also an important factor affecting the fermentation process of ethanol. Acid conditions are more suitable for yeast fermentation [ 27 ]. The activity of the microbial enzyme has a constant pH adaptation range.…”

Section: Resultsmentioning

confidence: 99%

Ethanol Production from Colpomenia sinuosa by an Alginate Fermentation Strain Meyerozyma guilliermondii

Mao

Liu

Wang³

2021

Indian J Microbiol

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“…Some strains can also utilize dextran, glucose and mannitol to produce ethanol, which are released from brown algae after pretreatment with acids or enzymes (Adams et al 2009; Lee and Lee 2012). Zhang et al (2014) reported a strain that can directly utilize alginate to produce ethanol. To date, only a few wild strains have been reported to simultaneously utilize the main components of brown algae, including alginate, mannitol, laminarin, fucose and glucose, as carbon sources to produce ethanol (Ji et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2

et al. 2019

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ObjectivesBifunctional alginate lyase can efficiently saccharify alginate biomass and prepare functional oligosaccharides of alginate.ResultsA new BP-2 strain that produces alginate lyase was screened and identified from rotted Sargassum. A new alginate lyase, Alg17B, belonging to the polysaccharide lyase family 17, was isolated and purified from BP-2 fermentation broth by freeze-drying, dialysis, and ion exchange chromatography. The enzymatic properties of the purified lyase were investigated. The molecular weight of Alg17B was approximately 77 kDa, its optimum reaction temperature was 40–45 °C, and its optimum reaction pH was 7.5–8.0. The enzyme was relatively stable at pH 7.0–8.0, with a temperature range of 25–35 °C, and the specific activity of the purified enzyme reached 4036 U/mg. A low Na+ concentration stimulated Alg17B enzyme activity, but Ca2+, Zn2+, and other metal ions inhibited it. Substrate specificity analysis, thin-layer chromatography, and mass spectrometry showed that Alg17B is an alginate lyase that catalyses the hydrolysis of sodium alginate, polymannuronic acid (polyM) and polyguluronic acid to produce monosaccharides and low molecular weight oligosaccharides. Alg17B is also bifunctional, exhibiting both endolytic and exolytic activities toward alginate, and has a wide substrate utilization range with a preference for polyM.ConclusionsAlg17B can be used to saccharify the main carbohydrate, alginate, in the ethanolic production of brown algae fuel as well as in preparing and researching oligosaccharides.

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“…In order to improve the utilization rate of algae, high alginate fermentation strains should be obtained not only from gene engineering technology but also from natural isolation. In our previous research 21 , alginate degrading strains were isolated from different samples with alginate as the only carbon source and several strains that showed ethanol fermentation ability were obtained through TTC selection experiments and fermentation experiments. The ethanol yield of strain 5 was the highest, which was 0.154 g g -1 (ethanol to alginate).…”

Section: Introductionmentioning

confidence: 99%

Enzyme Detection and Metabolic Process Tracking of Ethanol Fermentation by a Natural Alginate Fermentation Strain

Ren

LiYin

2018

Braz. arch. biol. technol.

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Alginate is a major component of brown algae, but it cannot be utilized for ethanol fermentation by industrial microorganisms. A natural alginate degrading and ethanol producing strain was obtained in our previous research. However, the research on the ethanol metabolism process of the natural alginate fermentation strain is lacked. In this research, the key enzyme and metabolic process of ethanol fermentation were studied. Three kinds of key enzyme including alginate lyase, pyruvate dehydrogenase and ethanol dehydrogenase were determined. The enzyme activity in the metabolic process was relatively high at 60-96 h which was the most important period during the fermentation. Meanwhile the concentration change of the important substances including soluble sugar, reducing sugar, acidity, pyruvic acid and ethanol were tracked and analyzed. Total soluble sugar and reducing sugar change tendency during the fermentation was similar. In the whole fermentation process, the fermentation broth was acidic. The value of pyruvic acid content reached highest at 72 h. During 48-96 h, the growth of ethanol concentration was very obvious. The alginate metabolic process in natural alginate fermentation strain was to generate extracellular alginate lyase to degrade alginate to produce reducing sugar, and then some intermediate metabolites formed such as pyruvic acid. Finally under the effect of pyruvate dehydrogenase and ethanol dehydrogenase, ethanol was produced.

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The Isolation and Performance Studies of an Alginate Degrading and Ethanol Producing Strain

Cited by 20 publications

References 23 publications

Ethanol Production from Colpomenia sinuosa by an Alginate Fermentation Strain Meyerozyma guilliermondii

Ethanol Production from Colpomenia sinuosa by an Alginate Fermentation Strain Meyerozyma guilliermondii

Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2

Enzyme Detection and Metabolic Process Tracking of Ethanol Fermentation by a Natural Alginate Fermentation Strain

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