The Isolation and Some Properties of Radiation-sensitive Mutants of Micrococcus radiodurans

Moseley, Bevan

doi:10.1099/00221287-49-2-293

Cited by 66 publications

(44 citation statements)

References 21 publications

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“…The radiation resistance values obtained in this study for Deinococcus radiodurans were comparable to those obtained previously for this organism (32,33). The different radiation resistance of strains AG3aT and ALT-lbT may not reflect a taxonomic distinction between the two species, but may be due to differences among D. radioduransl strains, as was shown for several strains assigned to Deinococcus radiopugnans (28).…”

Section: Discussionsupporting

confidence: 77%

Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., Two Extremely Radiation-Resistant and Slightly Thermophilic Species from Hot Springs

Ferreira

Nobre

Rainey

et al. 1997

International Journal of Systematic Bacteriology

215

206

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Strains ofThe genus Deinococcus includes the species Deinococcus radiodurans, Deinococcus proteolyticus, Deinococcus radiophilus, and Deinococcus radiopugnans. The species Deinococcus erythromyxa was also included in this genus as a species incertae sedis by Brooks and Murray (3). The rod-shaped organisms of the species Deinococcus grandis are very closely related to the species of the genus Deinococcus, but were classified in the genus Deinococcus primarily on the basis of morphology (36). Recently, the type strains of these species were subjected to a complete 16s ribosomal DNA (rDNA) sequence analysis, which resulted in the reclassification of Deinococcus grandis and Deinococcus erythromyxa (41). The species Deinococcus grandis, despite its rod-shaped morphology, falls within the radiation of the genus Deinococcus and for this reason was classified as Deinococcus grandis. The species Deinococcus erythromyxa was known by Brooks and Murray (3) to have characteristics that were different from those of the other species of Deinococcus, and subsequent studies confirmed its chemotaxonomic distinctiveness, which reinforced the need for reclassification of this species (12). The species Deinococcus erythromyxa has been found to be phylogenetically very closely related to the gram-positive species Kocuria rosea, but due to the low DNA-DNA hybridization values between the two species, Kocuria erythromyxa was maintained as a separate speciesThe species of the genus Deinococcus are strictly aerobic, have optimum growth temperatures in the range from 25 to 35"C, produce reddish colonies, generally stain gram positive, have ornithine in the peptidoglycan, lack teichoic acids, possess menaquinone 8 as the major respiratory quinone, and are (41).

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Section: Discussionsupporting

confidence: 77%

Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., Two Extremely Radiation-Resistant and Slightly Thermophilic Species from Hot Springs

Ferreira

Nobre

Rainey

et al. 1997

International Journal of Systematic Bacteriology

215

206

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“…The 1-kb DNA ladder was composed of 10 DNA fragments (1,2,3,4,5,6,7,8,9, and 10 kbp; Takara Bio). The 100-bp DNA ladder (Takara Bio) was composed of 11 DNA fragments (0.…”

Section: Methodsmentioning

confidence: 99%

Interaction of double‐stranded DNA with polymerized PprA protein from Deinococcus radiodurans

et al. 2014

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Pleiotropic protein promoting DNA repair A (PprA) is a key protein that facilitates the extreme radioresistance of Deinococcus radiodurans. To clarify the role of PprA in the radioresistance mechanism, the interaction between recombinant PprA expressed in Escherichia coli with several double-stranded DNAs (i.e., super coiled, linear, or nicked circular dsDNA) was investigated. In a gel-shift assay, the band shift of supercoiled pUC19 DNA caused by the binding of PprA showed a bimodal distribution, which was promoted by the addition of 1 mM Mg, Ca, or Sr ions. The dissociation constant of the PprA-supercoiled pUC19 DNA complex, calculated from the relative portions of shifted bands, was 0.6 lM with Hill coefficient of 3.3 in the presence of 1 mM Mg acetate. This indicates that at least 281 PprA molecules are required to saturate a supercoiled pUC19 DNA, which is consistent with the number (280) of bound PprA molecules estimated by the UV absorption of the PprA-pUC19 complex purified by gel filtration. This saturation also suggests linear polymerization of PprA along the dsDNA. On the other hand, the bands of linear dsDNA and nicked circular dsDNA that eventually formed PprA complexes did not saturate, but created larger molecular complexes when the PprA concentration was >1.3 lM. This result implies that DNAbound PprA aids association of the termini of damaged DNAs, which is regulated by the concentration of PprA. These findings are important for the understanding of the mechanism underlying effective DNA repair involving PprA.

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“…2). Radiation-resistant bacterial strains including D. radiodurans, D. geothermalis, D. murrayi, R. radiotolerans and R. xylanophilus have exhibited variable radiation resistance in previous studies (Moseley, 1967;Moseley & Mattingly, 1971;Moseley & Evans, 1983;Ferreira et al, 1997;Suzuki et al, 1988;Yoshinaka et al, 1973;Carreto et al, 1996). Of these radiation-resistant bacteria, the thermophilic species D. murrayi exhibited stronger resistance to gamma-radiation (shoulder doses, 7?3 kGy) than did the well-known species D. radiodurans (shoulder doses, 5?0 kGy) and would be the most radiation-resistant bacterium currently known.…”

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confidence: 99%

Rubrobacter taiwanensis sp. nov., a novel thermophilic, radiation-resistant species isolated from hot springs

Chen

Lin

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

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Two novel bacteria, with an optimum growth temperature of approximately 60 6C, were isolated from Lu-shan hot springs in the central region of Taiwan. These isolates were aerobic, thermophilic, halotolerant, pink-pigmented, heterotrophic and resistant to gamma-radiation. Both pleomorphic, short, rod-shaped cells and coccoid cells were observed. Strains LS-286 (=ATCC BAA-452=BCRC 17198) and LS-293 T (=ATCC BAA-406 T =BCRC 17173 T ) represented a novel species of the genus Rubrobacter, according to a phylogenetic analysis of the 16S rRNA gene, DNA-DNA hybridization, biochemical features and fatty acid composition. The name Rubrobacter taiwanensis sp. nov. is proposed for this novel species, with LS-293 T as the type strain.

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The Isolation and Some Properties of Radiation-sensitive Mutants of Micrococcus radiodurans

Cited by 66 publications

References 21 publications

Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., Two Extremely Radiation-Resistant and Slightly Thermophilic Species from Hot Springs

Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., Two Extremely Radiation-Resistant and Slightly Thermophilic Species from Hot Springs

Interaction of double‐stranded DNA with polymerized PprA protein from Deinococcus radiodurans

Rubrobacter taiwanensis sp. nov., a novel thermophilic, radiation-resistant species isolated from hot springs

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