The isolation and tentative identification of diacylglyceryl ethers from the walker 256 carcinoma of the rat and a human lymphosarcoma

Bollinger, James N.

doi:10.1007/bf02530914

Cited by 19 publications

(7 citation statements)

References 11 publications

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“…The occurrence of an unidentified neutral lipid in tumor tissue was first reported by Bollinger (1) and Cheng et al (2) and later confirmed (3,4). The lipid, isolated from Ehrlich ascites cells, was subsequently identified as a glyceryl ether diester (GEDE) (5).…”

Section: Introductionmentioning

confidence: 94%

Tumor lipids: Carbon number distribution of triglycerides and glyceryl ether diesters

Wood

Harlow

1970

Lipids

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Triglycerides and glyceryl ether diesters were isolated from a number of transplantable rat and mouse tumors and analyzed intact by gas liquid chromatography (GLC). The carbon number distributions of the triglycerides were distinctly different from those of the glyceryl ether diesters. Both glyceride classes contained abnormally high molecular weight species. The data support earlier evidence that indicated diglycerides used for the biosynthesis of phosphatides are also precursors of triglycerides in neoplasms. Glycerides isolated from three centrifuged fractions of Ehrlich ascites cells exhibited similar carbon number distributions. The results indicate a random distribution of triglyceride and glyceryl ether diester molecular species among cellular organeiles. Triglycerides, glyceryl ether diesters and neutral plasmalogens of the same carbon number were partially resolved by GLC.

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Section: Introductionmentioning

confidence: 94%

Tumor lipids: Carbon number distribution of triglycerides and glyceryl ether diesters

Wood

Harlow

1970

Lipids

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“…The lipid phase of fraction I methanolysates might also contain labeled cholesterol and labeled glycerol ether. Since the alkyl-DHAP is reduced by NADPH in brain (16), its dephosphorylation could lead to the presence of labeled glycerol ether in the lower layer, although Experiments from several laboratories (17,18) (20,21). It has recently been shown in brain that acyl-DHAP is a precursor of alkyl-DHAP and that the latter is reduced to alkyl-glycerolphosphate via NADPH (16).…”

Section: Discussionmentioning

confidence: 99%

The Acyl Dihydroxyacetone Phosphate Pathway for Glycerolipid Biosynthesis in Mouse Liver and Ehrlich Ascites Tumor Cells

Agranoff

Hajra

1971

Proc. Natl. Acad. Sci. U.S.A.

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The glycerol portion of lipids may be derived biosynthetically by reduction of dihydroxyacetone phosphate to glycerolphosphate, and then be acylated with fatty acids or, alternatively, dihydroxyacetone phosphate may first be acylated and then reduced to I-acyl sn glycerol-3-phosphate. Since the former pathway utilizes NADH for reduction of the C-2 carbonyl, while the latter requires NADPH, we were able to compare the relative participation of the two pathways for phospholipid synthesis by measuring the incorporation of radioactivity from tritiumlabeled NADH and NADPH into C-2 of lipid glycerol. The acyl-dihydroxyacetone phosphate pathway plays a significant role in glycerolipid synthesis in mouse liver homogenates and a clearly dominant one in Ehrlich ascites tumor cell homogenates. This finding is related to a reported lack of glycerol-3-phosphate dehydrogenase in tumor cells and to their high glycerol ether lipid content.

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“…5B). DISCUSSION Several investigators have noted an association between elevated neutral ether-lipid content and a variety of spontaneously arising and transplantable tumors in animals and man (2,(4)(5)(6)(7)(8)(9). These ether-linked lipids are distinct from the plasmalogens (alk-1-enyl lipids), which are relatively common in normal mammalian tissue and also occasionally are elevated in tumors (27,28).…”

mentioning

confidence: 98%

“…Several investigators have found elevated levels of an unusual class of compounds, neutral ether-linked lipids, in a variety of tumors in animals and man (4)(5)(6)(7)(8)(9). Certain cultured cell lines of different origins that contain high ether-lipid levels have been found to be tumorigenic (10)(11)(12)(13), but little information has been available on the tumorigenicity of closely related cell lines that differ primarily in lipid content, so that the significance of this variable could be evaluated.…”

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confidence: 99%

Tumorigenicity of cell lines with altered lipid composition.

Roos¹,

Choppin²

1984

Proc. Natl. Acad. Sci. U.S.A.

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A series of closely related mouse fibroblast cell lines that differ in their content of neutral ether-linked glycerolipid and fatty acids has been used to investigate the relationship between lipid composition and tumorigenicity. Although these cell lines, derived from the same parental culture, were selected without reference to transformation or tu. morigenicity, their ability to form tumors in irradiated mice was found to be closely correlated with ether-lipid content. The cell line with the highest level of ether-lipid (designated F40) produces more tumors, the tumors appear more rapidly than when parental cells are injected, and the number of F40 cells required for tumor induction is less by a factor of lO000. A large body of literature has suggested an association between lipids and tumors (1-3). Several investigators have found elevated levels of an unusual class of compounds, neutral ether-linked lipids, in a variety of tumors in animals and man (4-9). Certain cultured cell lines of different origins that contain high ether-lipid levels have been found to be tumorigenic (10-13), but little information has been available on the tumorigenicity of closely related cell lines that differ primarily in lipid content, so that the significance of this variable could be evaluated.In experiments designed to investigate the mechanism of cell fusion, we previously developed (from a culture of mouse L-cell fibroblasts) a series of cell lines that are highly resistant to the fusogenic effects of polyethylene glycol (PEG) (14,15). Biochemical analysis of these cells has revealed several alterations in lipid content that appear to be directly responsible for the fusion response in these cells (ref. 16; unpublished data). Among the biochemical features associated with the PEG-resistant phenotype is a >30-fold increase in neutral ether-lipid content. In addition to the parental and the most highly lipid-altered cell lines, the selection procedure produced a graded series of homogeneous, genetically stable cell lines that span the two extremes with respect to ether-lipid content and fusibility (15,17).The progressively altered lipid composition of these cells provides a system for studying the association between neutral ether-linked lipids and tumorigenicity. These PEG-resistant cells were derived from a single culture of parental cells without reference to their degree of transformation in vitro or tumorigenicity in vivo (15). If ether-linked lipids are indeed a significant factor in tumorigenicity, then these cells might be expected to form tumors in proportion to their content of ether-lipid. We report here a direct correlation between ether-lipid content and the ability to form tumors in mice. Features of increased malignancy acquired by these cells in parallel with their acquisition of an altered lipid content include increased invasiveness into adjacent tissues, ability to metastasize, and decreased tumor regression. MATERIALS AND METHODSCells and Media. The isolation and characterization of PEG-resistant der...

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The isolation and tentative identification of diacylglyceryl ethers from the walker 256 carcinoma of the rat and a human lymphosarcoma

Cited by 19 publications

References 11 publications

Tumor lipids: Carbon number distribution of triglycerides and glyceryl ether diesters

Tumor lipids: Carbon number distribution of triglycerides and glyceryl ether diesters

The Acyl Dihydroxyacetone Phosphate Pathway for Glycerolipid Biosynthesis in Mouse Liver and Ehrlich Ascites Tumor Cells

Tumorigenicity of cell lines with altered lipid composition.

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