The Isolation of Cotton Plant Components by High-Performance Liquid Chromatography

Abstract

The use of high-performance liquid chromatography (HPLC) to separate components of extracts of cotton bracts is described. A chloroform extract of the aqueous extract of the bracts and an ethanol extract of the dried aqueous extracts were used. The facile separation of 1,7-dihydroxy-4-isopropyl-1,6-dimethyl-2(1H)-naphthalenone (lacinilene C), its 7 methyl ether, and β-sitosterol from the chloroform extract, and 7-hydroxy-6-methoxycoumarin (scopoletin), cinnamic acid, and hydroxy cinnamic acid from the ethanol extract illustrate the capability of HPLC to separate the complex mixture of compounds in cotton plant parts. Preliminary separations by preparative thin-layer chromatography gave several major bands, but the HPL chromatograms showed that each band contains the same compounds, though in varying concentration, demonstrating the higher resolution of HPLC. Scopoletin was a major component in both extracts. Cinnamic and hydroxy cinnamic acids were identified by GC/MS. The other compounds were identified by pmr spectroscopy.

Secondary Metabolites of Gossypium: A Biogenetic Analysis

“…Much of such material is found in the seeds and in the waxes of the leaves and much is tied up as glyceride or as sterol ester (6). Of these fatty acids, palmitic (12), linoleic (21), oleic (19) and stearic (18) acids are the predominant ones. Fatty acid substances having an odd number of carbons are generally less common; several appear in Gossypium.…”

“…Studies of Gossypium metabolites have uncovered the occurrence of a few plant sterols, including the representative gsitosterol (219) (8,18,47,59). These widely occurring phytosterols arise by early formation of cycloartenol (not yet reported) , which is then structurally modified.…”

Occurrence of Nitrogen-Fixing Bacteria on Bracts, Locules, and in Cotton Dust

“…The inorganic and organic contents in the dust and, more specifically, the compounds such as histamines, polyphenols, indole-3-carboxaldehyde, scopletin, etc., are also reported to be responsible [3][4][5][6][7][8][9]14]. Among the bacterial species reported to be present and significantly contributing to the level of endotoxins is Enterobacter agglomerans [ 16].…”