The isolation of high molecular weight DNA from whole organisms or large tissue masses

Heterozygote deficiency and chimerism in remote populations of a colonial ascidian from New Zealand

“…Samples from the field were placed, separately, into 1.5 ml vials containing 240 µl lysis buffer (Graham 1978), homogenized, and extracted with 240 µl phenol/chloroform. The material was shipped to the laboratory in Israel, where DNA isolation was completed by additional phenol/chloroform and chloroform extraction.…”

Population genetics of the invasive ascidian Botryllus schlosseri from South American coasts

“…DNA extraction and microsatellite typing of colonies were performed as per . Samples from the field were placed separately into vials containing lysis buffer (Graham 1978), homogenized and extracted with phenol/chloroform. Five Botryllus schlosseri microsatellites, BS-811 (Pancer et al 1994), PB-29, PB-41, PB-49 and PBC-1 (Stoner et al 1997), were used following primers and suggested conditions.…”

Reiteration frequency of genes coding for abundant and rare messenger RNA in greened barley seedlings

“…Tissue was broken and DNA extracted as described by Graham (1978). DNA was further purified according to the UP procedure described by Britten etal.…”