The isolation of hybrid cell lines producing monoclonal antibodies against the p15(E) protein of ecotropic murine leukemia viruses

Nowinski, Robert C.; Lostrom, Mark E.; Tam, Milton R.; Stone, Mary R.; Burnette, W N

doi:10.1016/0042-6822(79)90280-0

Cited by 319 publications

(125 citation statements)

References 26 publications

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“…Although after further growth in 24-well plates most of them lost their activity (Nowinski et al, 1979;Ozato et al, 1980), in the early screening about 500 of positive supernatants were obtained (specific 51Cr-release 15-60%). One of 3 definitely positive hybrids, immediately minicloned, Nicolin et al (1974).…”

Section: Resultsmentioning

confidence: 99%

“…at 5 cells/well (Nowinski et al, 1979), has yielded 95% positive hybrids. Cell culture were allowed to grow on a larger scale and supernatants were collected and frozen at -80°C.…”

Section: Resultsmentioning

confidence: 99%

“…Hybridization.-The hybridization technique described by Nowinski et al (1979) has been followed, with minor modifications. Briefly, CD2F1 mice were challenged with 107 viable L1210/DTIC cells i.p.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of a monoclonal antibody to L1210 leukaemia

Testorelli¹,

Morelli²,

Goldin³

et al. 1982

Br J Cancer

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Summary.-A mouse monoclonal cell line (L1) was produced by fusing the mouse myeloma P3X63/Ag8 with CD2F1 spleen cells immunized with a highly immunogenic subline of L1210 leukaemia (Li210/DTIC). A very few positive clones (1%) were isolated and one of these was chosen for detailed study. The monoclonal antibody Li is an IgM immunoglobulin strongly reacting in a complement-dependent cytotoxicity assay against L1210/Cr leukaemia and its more or less inmmunogenic sublines. The specificity of the LI antibody against L1210 leukaemia was studied by extensive screening with normal adult and foetal tissues, lymphoid tissues from several independent strains and a panel of the most common experimental tumours, to all of which it was unreactive. Attempts at immunotherapy were carried out in DBA/2 mice challenged with L1210 leukaemia and treated with Li (ascites) and complement. Although the in vitro cytotoxic titre of ascites fluid from mice bearing hybridoma was very high (10-7), no therapeutic effect was obtained in vivo.

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Section: Resultsmentioning

confidence: 99%

“…at 5 cells/well (Nowinski et al, 1979), has yielded 95% positive hybrids. Cell culture were allowed to grow on a larger scale and supernatants were collected and frozen at -80°C.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of a monoclonal antibody to L1210 leukaemia

Testorelli¹,

Morelli²,

Goldin³

et al. 1982

Br J Cancer

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show abstract

“…Cell fusion with the mouse myeloma NS/1 was performed 3 days after the last immunization. Hybrids producing antibodies were screened and minicloned in microtiter wells as in [17,18]. Two hybridoma clones were isolated; these are called D5 and H5, respectively.…”

Section: Production Of Hybridomasmentioning

confidence: 99%

“…The specificity of two hybridoma antibodies in their reactivity to different domains isolated after proteolysis was determined first by plate assay [17,18]. The antibody produced by the hybridoma D5 reacted specifically with the gelatin-binding domain with 40 000 M r. In contrast, the antibody produced by the hybridoma H5 reacted specifically with the 150 000-140 000 M r fragment which represents the domain capable of binding to heparin and inducing cell attachment and spreading.…”

Section: Domain Specificity Of Two Hybridoma Antibodiesmentioning

confidence: 99%