The isolation of lamellar bodies and their membranous content from rat lung, lamb tracheal fluid and human amniotic fluid

Duck-Chong, C G

doi:10.1016/0024-3205(78)90549-0

Cited by 69 publications

(41 citation statements)

References 15 publications

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“…The morphometric results of Table 2 agree with the biochemical estimates of lamellar body pool size and indicate a small rise in the lamellar body pool after birth in unlavaged lungs. It is possible that the lamellar body isolation procedures result in contamination of the isolates with extracellular DSPC (8). If true, this would result in an overestimate of the lamellar body pool.…”

Section: Resultsmentioning

confidence: 99%

“…The Annual Meeting of the European Society for Pediatric Research will be held in Padova, Italy, September [6][7][8][9][10]1987 …”

Section: Annual Meetingmentioning

confidence: 99%

“…The alveolar epithelium is still immature at birth in the rat; to the method of Duck-Chong (8). The lavage samples were spun we chose to study this animal model because of the predictability in increasing centrifugal force fields, according to the expansion of its gestational age and because of the relatively immature state of the technique of Thet et al (9) given by Magoon, et 01.…”

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confidence: 99%

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Alterations of Surfactant Pools in Fetal and Newborn Rat Lungs

1987

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ABSTRACT. Preparation by the developing alveolar epithelium for the transition to air breathing and surfactant secretion at birth are critical components of neonatal survival. We combined morphometric analysis and biochemical assays of lung phospholipids to measure the amount and redistribution of lung surfactant during the perinatal period of rats. Within 10 min of the start of air breathing, there was a small increase in type I1 cell lamellar body content by morphometric and biochemical estimates. By 24 h, the whole lung and alveolar extracellular pool surfactant lipid had substantially increased. Subfractionation of the alveolar surfactant pool obtained at four times, from birth to 24 h of life, demonstrated a 20-fold increase in the ratio of phospholipid in a tubular myelin-rich fraction compared to a unilamellar vesicle-rich fraction. We conclude that packaging of surfactant may be very active immediately postbirth. Our results also indicate a major shift in the physical forms of extracellular surfactant during the first hours of air breathing. (Pediatr Res 21: 5-9, 1987) Abbreviations quantifying changes in the intracellular and extracellular pools of surfactant-associated phospholipid in the late fetal and newborn period, using morphometric and biochemical assays. METHODS Animals.Timed pregnant (previously virgin) Sprague-Dawley females (Zivic-Miller, Allison Park, PA) were received 5 to 7 days prior to parturition. During their late stages of pregnancy, they were allowed food and water ad libitum and were kept in 12-h light-dark cycles. The onset of labor was defined as behavior including nest building and agitation along with vaginal bleeding. Birth began shortly after nesting was complete and was allowed to progress uninterrupted until three to six fetuses were delivered. The air-breathing newborns were kept with the unanesthetized mother. The mothers were then anesthetized with 30-50 mg/kg pentobarbital intraperitoneally; the remaining fetuses were delivered individually through a hysterotomy. These fetuses (birth group) were more lethargic, but made limb motions. They were still connected to the placenta and made no respiratory efforts. The mothers continued spontaneous breathing during the entire procedure. DSPC, disaturated phosphatidylcholineNo more than 10 min passed before the newborns (+lo min PL, phospholipid group) were also anesthetized with intraperitoneal pentobarbital, LDH, lactate dehydrogenase the thoracic contents were rapidly removed and lung wet weight was obtained. Two or three unlavaged lungs were pooled for the LB DSPC' lamellar body disaturated phosphatidylcholine ~h o s~h o l i p i d determinations in all fetal animals. Pooled s a m~l e s -A -from these animals were regarded as a single determination. Since the +10 min group were expected to have developed in the lower poles of the uterus, we obtained lungs from fetuses in Immediately after birth, the newborn must transition to air the two lowest and the two highest uterine positions of four breathing and the effects this has on...

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Section: Resultsmentioning

confidence: 99%

“…The Annual Meeting of the European Society for Pediatric Research will be held in Padova, Italy, September [6][7][8][9][10]1987 …”

Section: Annual Meetingmentioning

confidence: 99%

mentioning

confidence: 99%

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Alterations of Surfactant Pools in Fetal and Newborn Rat Lungs

1987

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“…It may have biophysical significance in stomata together with guard-cell wall thickening and membraneassociated cytoskeletal elements, providing a piezobiophysical buttress to prevent bursting under conditions of low turgor. The analogy should, however, not be taken too far since there is no evidence that guard cells are coated with a DPPC containing monolayer as occurs at the air:liquid interface of pulmonary tissue (Sanders & Longmore, 1975;Duck-Chong, 1978;Harwood, 1987). As here envisaged DPPC in guard cells is an integral plasma membrane-contained PL constituent providing mechanical support.…”

Section: Discussionmentioning

confidence: 89%

A biophysical study of abscisic acid interaction with membrane phospholipid components

et al. 1990

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SUMMARYMolecular area/surface pressure Langmuir isotherms of amphiphilic dipalmitoylphosphatidylcholine (DPPC) monolayers indicated that abscisic acid (ABA) has a marked rigidifying effect, expressed as reduction of molecular area and increase of monolayer collapse point. Moreover, ABA markedly increased aqueous droplet hydrophobicity, as indicated by a concentration-dependent increase of contact angle when placed on a hydrocarbon chain surface; no such effects were obtained on either amphiphilic or octadecyltrichlorosilane surfaces. A combination of TLC and mass spectometry revealed the presence of DPPC in Vicia faba and Commelina communis guard-cell protoplast membranes. ABA also increased plasma membrane rigidity as evidenced by probing with lipid specific membrane probes, namely diphenylhexatriene and its trimethyl derivative. Regarded together the results suggest a specific site of ABA binding to DPPC. The linkage between senescence and stomatal closure is discussed in the light of the new data presented here. It is suggested that DPPC in guard-cell membranes may have a physical role in preventing collapse and/or bursting. In this connection an analogy is drawn with pulmonary mechanisms.

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“…The the risk of fetal death in pregnancies comp,icated by severe blood was collected into heparinized tubes and after centrifugahypertension-edema-proteinur~a syndromes ( 7). Hence, other tion, the plasma was stored at -20°C until required for analysis.…”

Section: T4 Thyroxinementioning

confidence: 99%

The Effect of Intra-amniotic Injection of Triiodothyronine on Pulmonary Maturity in Lambs at 130 Days Gestation

et al. 1984

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Summaryistered intra-amniotically rather than maternally, because the sheep placenta is only very slightly permeable to T3 (8) and it Saline or triiodoth~ronine (T3) (50-1000 rg) was injected into has been shown that thyroid is readily transferred from the amniotic sac of 17 pregnant ewes under general anesthesia at 130 davs gestation. Fortv-eight h later. the lambs were deliv-the amniotic fluid to the fetal circulation (26).-< , ered by hyst&otomy. Cord plasma T3 and cortisol and amniotic fluid T3 were assayed, and the maturity of the fetal lung was MATERIALS AND METHODSassessed in terms of its pressure-volume response and its surfacTrearmenr and lambs. seventeen sheep ( 13 pure bred rant (lamellar body phospholipid) content. With the highest dose ~~r i~~ ewes and four D~~~ ~~~~/~ cross-bred ewes) of TJ, cord ~lasma T3 and cortisol were raised, and lung maturity were used. Pregnancies were achieved by single matings after the was F3hanced compared with saline-treated conh.0ls; the Pres-induction of estrus, and by test mating. ~t 1 16 days sure-volume curve showed increased hysteresis on inflation and gestation, the pregnant ewes were transported from open pasture deflation, and the lung retained air on return to Zero Pressure. to the university animal house for acclimatization, where they There was also an apparent, but not statistically significant, were maintained in individual pens and fed lucerne chaff. The increase in the lamellar body phospholipid content of the lung. experiments were conducted in a blind fashion where T3 (20 pg/ ~rres~ective of treatment, lungs which were more mature, in ml saline) and saline controls were packaged identically. The terms of their p r e~~~r e -v o~~m e characteristics, tended to contain code was broken at the end of experimentation on a group of a higher proportion of lamellar body phospholipid relative to animals so that an ongoing analysis of the results was possible, total phospholipid. and modification of the protocol could be camed out until an appropriate dose of T3 was achieved. At 130 days gestation, Abbreviations under general anesthesia (after 12 h fasting), a lower paramedian incision was made in the abdomen, and the uterus was exposed. T4 thyroxineThe fetal neck was grasped and 20 ml of amniotic fluid was T* triiodothyronine withdrawn. T3 (50-1000 pg) or saline was injected by the same needle, so that it was introduced as close as possible to the fetal pregnant threatening premature delivery are often mouth. The incision was closed, and the animals were returned treated with glucocorticoid in an attempt to accelerate matunto their cages with only local antibiotic cover to their incision. tion of the fetal lungs and thus avoid hyaline membrane disease After 48 h, the lambs were delivered by hysterotomy under in the neonate (17). However, the treatment is not always eflec-general anesthesia.the lamb, 20 ml of tive and early results suggested that such treatment may increase fluid and l o ml were taken. The the risk of fetal death in pregnancies comp,icated by se...

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The isolation of lamellar bodies and their membranous content from rat lung, lamb tracheal fluid and human amniotic fluid

Cited by 69 publications

References 15 publications

Alterations of Surfactant Pools in Fetal and Newborn Rat Lungs

Alterations of Surfactant Pools in Fetal and Newborn Rat Lungs

A biophysical study of abscisic acid interaction with membrane phospholipid components

The Effect of Intra-amniotic Injection of Triiodothyronine on Pulmonary Maturity in Lambs at 130 Days Gestation

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