The isolation of mycoplasma species from horses

Allam, N.; Powell, D. G.; Andrews, B. E.; Rm, Lemcke

doi:10.1136/vr.93.14.402

Cited by 13 publications

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“…The culture method has been used in many retrospective studies (3)(4)(5)7,10,12,24). In our study, both culture and PCR methods were used in order to detect M. felis and M. equirhinis, which were thought to be associated with IAD in horses.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the presence of Mycoplasma as an etiologic agent of inflammatory airway diseases in thoroughbred racehorses in İstanbul Province

Mete¹,

Özgür²

2017

Turk J Vet Anim Sci

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Mycoplasma equirhinis and Mycoplasma felis are thought to be infectious etiologic agents of inflammatory airway disease (IAD), which, after musculoskeletal injuries, is the second most common cause of poor performance in thoroughbred racehorses. The aims of this study were to investigate for the first time the presence of M. equirhinis, M. felis, and other Mycoplasma spp. in the lower respiratory tract of racehorses in İstanbul Province in Turkey and to statistically evaluate the association between clinical signs of IAD and the presence of mycoplasmas. Tracheal wash (TW) samples were collected from 111 English and Arabian thoroughbred horses that showed clinical signs of IAD. TW samples were examined by culture and PCR methods. The detection rates of Mycoplasma spp., M. felis, and M. equirhinis were found to be 16.2%, 0%, and 18% by culture and 59.5%, 1.8%, and 6.3% by PCR in TW samples, respectively. According to statistical evaluations, a significant association was found for the presence of M. felis in English thoroughbred racehorses. However, no significant association was found between clinical signs of IAD and the presence of M. equirhinis, M. felis, and other Mycoplasma spp.

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Section: Discussionmentioning

confidence: 99%

Investigation of the presence of Mycoplasma as an etiologic agent of inflammatory airway diseases in thoroughbred racehorses in İstanbul Province

Mete¹,

Özgür²

2017

Turk J Vet Anim Sci

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“…Several species of mycoplasmas have been isolated from the respiratory tracts of both healthy and diseased horses (Allam and others 1973, Windsor 1973, Allam and Lemcke 1975, Moorthy and Spradbrow 1976, Poland and Lemcke 1978), including Mycoplasma equirhinis and M felis , without clear evidence of pathogenic significance. However, M felis has been isolated from cases of pleuritis in horses and experimental infection with this organism induced pleuritis (Ogilvie and others 1983, Rosendal and others 1986, Hoffman and others 1992, Morley and others 1996).…”

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confidence: 99%

An outbreak of respiratory disease in horses associated withMycoplasma felisinfection

et al. 1997

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Lower respiratory tract disease developed in a group of racehorses in training between two and six years of age. Disease was observed in 22 of 25 horses for which full records were available. Seroconversion to Mycoplasma felis was demonstrated by indirect haemagglutination assay in 19 of 22 paired sera and high titres (> or = 64) were found in convalescent sera from the three remaining horses. Evidence of respiratory viral infection was confined to seroconversions to equine herpesvirus-4 in two of the horses. Tracheal wash samples, taken from four horses with visibly increased tracheal mucopus, contained more than 10(4) colony forming units/ml M felis and high proportions of neutrophils. This is the first description of an outbreak of lower respiratory tract disease in horses in training associated with M felis infection.

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“…Mycoplasmas and acholeplasmas have been reported to be present in the respiratory tract (1,2,6,14,18,20,24), the genital organs (15,20), the conjunctivae (20), and the joints (6) of horses. Mycoplasmas and acholeplasmas have also been encountered in aborted horse fetuses (13,16) and in stallion semen (7,8).…”

Section: Equigenitalim Is T37 ( = Atcc 29869 = Nctc 10176)mentioning

confidence: 99%

Mycoplasma equigenitalium, a New Species from the Cervix Region of Mares

Kirchhoff

1978

International Journal of Systematic Bacteriology

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Eleven glucose-fermenting Mycoplasma strains isolated h m the cervix region of mares exhibited serological properties which distinguished them from strains of the presently known Mycoplasma species. From the results obtained by growth inhibition, metabolic inhibition, indirect-immunofluorescence, and double-immunodiffUsion tests, it can be concluded that these strains belong to a new species, for which the name Mycoplasma equigenitalium is proposed. The type strain of M. equigenitalim is T37 ( = ATCC 29869 = NCTC 10176).Mycoplasmas and acholeplasmas have been reported to be present in the respiratory tract (1,2,6, 14, 18,20,24), the genital organs (15,20), the conjunctivae (20), and the joints (6) of horses. Mycoplasmas and acholeplasmas have also been encountered in aborted horse fetuses (13, 16) and in stallion semen (7,8).To date, a total of 10 distinct Mycoplusmatales species have been found in horses: Acholeplasma laidlawii, A. oculi, A. granularum, A. axanthum, A. equifetak, A. hippikon (lla, 12), Mycoplasma pulmonis, M. felis, M. bovigenitalium, and M. equirhinis, a recently described species from the respiratory tract of horses (1).The present report deals with the characterization and identification of 11 Mycoplasma strains previously isolated from the cervix region of mares (15). MATERIALS AND METHODSMycoplasma strains. The 11 Mycoplasma strains used in this study (T33, T37, T84, T121, T122, T145, T199, T267, T270, T344, and T349) were obtained from cotton swabbings of the cervix region of mares (15). The isolates were each cloned three times by picking single colonies. The fluid culture was passed through a filter with an average pore diameter of 450 nm, and dilutions of the filtrate were plated on a solid medium to produce well-separated colonies.Culture media; The strains were cultivated in a medium of the following composition: heart infusion broth (Oxoid), 720 ml, unheated horse serum, 200 ml, 25% (wt/vol) fresh yeast extract, 10 d, deoxyribonucleic acid (Sigma Chemical Co.), 0.02 g; 1.25% (wt/vol) thallium acetate, 10 ml; and penicillin, 2 X 10' IU.Solid medium was prepared by adding 10 g of agar (Oxoid) to the fluid medium.Morphological studies. Colonies were examined with a stereo microscope (Leitz). Cells from broth cultures were examined by dark-field and phase-contrast microscopy (Leitz).Filtration characteristics. Filterability of the strains was determined by using Swinnex-25 adapters and membrane filters (Millipore Corp.) with a pore diameter of 450 or 200 run.Reversion experiments. The strains were passaged seven consecutive times on substrate without penicillin and thallium acetate. Passages one to five, inclusive, were performed on solid media by sliding inverted agar blocks across the agar surfaces. In the sixth passage, the agar blocks were inoculated into noninhibiting fluid medium and, after incubation, the organisms were examined by dark-field microscopy. The seventh passage was made on solid medium. Differentiation of acholeplasmas from mycoplasmas. Inhibition of growth by sodi...

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The isolation of mycoplasma species from horses

Cited by 13 publications

References 0 publications

Investigation of the presence of Mycoplasma as an etiologic agent of inflammatory airway diseases in thoroughbred racehorses in İstanbul Province

Investigation of the presence of Mycoplasma as an etiologic agent of inflammatory airway diseases in thoroughbred racehorses in İstanbul Province

An outbreak of respiratory disease in horses associated withMycoplasma felisinfection

Mycoplasma equigenitalium, a New Species from the Cervix Region of Mares

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