The Isolation of Two Bluetongue Viruses From Healthy Cattle in Australia

George, TD St; Cybinski, D. H.; Della-Porta, A.J.; McPhee, Dale A.; Wark, Margaret C.; Bainbridge, M. H.

doi:10.1111/j.1751-0813.1980.tb02598.x

Cited by 48 publications

(16 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To initially compare VI and PCR methods, serial log 10 dilutions of Australian reference strains of BTV-1 or BTV-21, 16 which had been passaged in cell culture 3-5 times, were prepared in phosphate buffered gelatin saline (PBGS; pH7.4) and 50 μl mixed with 950 μl of normal extended semen. Two samples were spiked with undiluted cell culture fluid and duplicate samples with dilutions from 10 −1 to 10 −6 , providing a total of 28 samples spiked with BTV from cell culture fluid.…”

Section: Semen Samplesmentioning

confidence: 99%

Longitudinal study of the detection of Bluetongue virus in bull semen and comparison of real-time polymerase chain reaction assays

Davis

Walsh

et al. 2014

J VET Diagn Invest

View full text Add to dashboard Cite

Abstract. Infection with Bluetongue virus (BTV) is a significant impediment to the global movement of bovine semen. Repeat testing of blood from donor animals is specified in the World Organization for Animal Health (OIE) Manual for the export of semen from regions where BTV may be present. Screening of blood or semen samples has usually been carried out by virus isolation (VI) either by inoculation of chicken embryos followed by passage onto insect and mammalian cell cultures or in vivo inoculation of sheep followed by serology to detect seroconversion. Direct testing of semen for BTV would enable earlier release of semen samples and avoid repeat testing of the donor, as well as provide an option for releasing batches of semen that were collected without certification of the donor. Quantitative (real-time) reverse transcription polymerase chain reaction (qRT-PCR) assays overcome most of the limitations of other methods and have the potential to provide higher sensitivity. The present study compared 5 qRT-PCR assays, including 2 commercially available kits, for the detection of BTV in semen serially collected from 8 bulls over a period of 90 days after experimental infection. The results of the study show that at least one of the qRT-PCR assays is extremely reproducible and has both very high sensitivity and specificity to reliably detect all available serotypes. The preferred qRT-PCR gave consistently superior results to VI, sheep inoculation, and conventional RT-PCR. Therefore, the assay can be recommended for the screening of bovine semen for freedom from BTV.

show abstract

Section: Semen Samplesmentioning

confidence: 99%

Longitudinal study of the detection of Bluetongue virus in bull semen and comparison of real-time polymerase chain reaction assays

Davis

Walsh

et al. 2014

J VET Diagn Invest

View full text Add to dashboard Cite

show abstract

“…This was the first indication that viruses of the bluetongue serogroup were present in Australia and resulted in numerous studies to map the distribution of the virus and identify its vectors Dyce and Standfast 1979;St George 1982). Despite extensive sampling of both insects and cattle in northern and eastern Australia no further isolates of serotype 20 have been made although numerous isolations of four other serotypes were made (St George et al 1980; T. D. St George, unpublished data).…”

Section: Bluetongue Serogroupmentioning

confidence: 99%

“…Each has been isolated in Australia from domestic animals (Pascoe et al 1978;Doherty et al 1969;St George et al 1980), but only Ross River virus has been associated with human disease (Doherty 1972(Doherty , 1977.…”

Section: Diseasementioning

confidence: 99%

Isolation of Arboviruses from Insects Collected at Beatrice Hill, Northern Territory of Australia, 1974?1976

Ha¹,

Al²,

Td³

et al. 1984

Aust. Jnl. Of Bio. Sci.

View full text Add to dashboard Cite

Between October 1974 and May 1976, 57596 mosquitoes, 169957 Culicoides, 5923 Lasiohelea and 1043 phlebotomines were collected for virus isolation at Beatrice Hill (lat. 12�39'S.,long. 131�20'E.) in the Northern Territory of Australia. A total of 94 viruses belonging to 22 different serological groupings was isolated. The following species of insect yielded viruses which were identified and those viruses marked with an asterisk represent a new record of insect host:

show abstract

“…BLU-20 (St George et al 1978) was passed up to 10 times in BHK or Vero cells. Other BLU viruses, CSIRO 154 (BLU-21) and CSIRO 156 (BLU-l) (St George et al 1980) were passed three times in BHK cells, plaque purified twice in Vero cells and passed a further four times in BHK cells.…”

Section: Virusesmentioning

confidence: 99%

Group-specific and Type-specific Gel Diffusion Precipitin Tests for Bluetongue Virus Serotype 20 and Related Viruses

Sharp¹,

Littlejohns²,

George³

1988

Aust. Jnl. Of Bio. Sci.

View full text Add to dashboard Cite

Using antigens prepared from cell cultures infected by bluetongue (BLU) virus type 20 (BLU-20), and sera from cattle which had recovered from experimental infection by that virus, two distinct precipitin reactions were demonstrated by immunodiffusion. Two distinct gel diffusion precipitin tests were developed based on these reactions. The antigen of one was common to BLU-20 and two other Australian BLU isolates, CSIRO 154 (BLU-21) and CSIRO 156 (BLU-l). It was therefore concluded to be a group-specific test. The antigen of the second appeared to be unique to BLU-20. The test based on this antigen correlated well with the virus neutralization test for BLU-20 and it was therefore concluded to be type-specific.Similar methods applied to a virus of the Palyam (PAL) group demonstrated two precipitin reactions of similar broad (group) and narrow (type) specificity.

show abstract

The Isolation of Two Bluetongue Viruses From Healthy Cattle in Australia

Cited by 48 publications

References 3 publications

Longitudinal study of the detection of Bluetongue virus in bull semen and comparison of real-time polymerase chain reaction assays

Longitudinal study of the detection of Bluetongue virus in bull semen and comparison of real-time polymerase chain reaction assays

Isolation of Arboviruses from Insects Collected at Beatrice Hill, Northern Territory of Australia, 1974?1976

Group-specific and Type-specific Gel Diffusion Precipitin Tests for Bluetongue Virus Serotype 20 and Related Viruses

Contact Info

Product

Resources

About