The isopentenyl transferase gene is effective as a selectable marker gene for plant transformation in tobacco ( Nicotiana tabacum cv. Petite Havana SRI)

Endo, Saori; Kasahara, Takehide; Sugita, Koichi; Matsunaga, Etsuko; Ebinuma, Hiroyasu

doi:10.1007/s002990000279

Cited by 50 publications

(25 citation statements)

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“…Ipt, a cytokinin biosynthetic gene cloned from Agrobacterium, has also been shown to stimulate shoot regeneration (Li et al 1992;Owens 1988, 1989;Strabala et al 1989Strabala et al , 1996. Further studies have shown the ipt gene may be used as a positive selectable marker for transformation in citrus, tobacco, rice, sweet pepper, aspen and apricot (Ballester et al 2007(Ballester et al , 2008Ebinuma and Komamine 2001;Ebinuma et al 1997;Endo et al 2001Endo et al , 2002Kunkel et al 1999;López-Noguera et al 2009;Mihálka et al 2003;Peng et al 2015). Similar to the ipt gene, we have previously reported that the kn1 gene can be used as a positive selection marker for transgenic tobacco plants , The utility of the kn1 gene in genetic transformation of higher plants has also been shown in Phalaenopsis amabilis and Jatropha curcas (Pei et al 2010;Semiarti et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Kn1 gene overexpression drastically improves genetic transformation efficiencies of citrus cultivars

Xie

et al. 2016

Plant Cell Tiss Organ Cult

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The effects of a maize knotted1 (kn1) gene under the control of the cauliflower mosaic virus 35S promoter on genetic transformation efficiencies of six citrus genotypes were tested. The kn1 gene construct was used to transform 'Pineapple', 'Hamlin', 'Sucarri' andOur results demonstrate that expression of the kn1 gene enhances transformation efficiencies from 3 to 15 fold compared to a control vector, 3-11 fold relative to the highest transformation efficiencies reported for these citrus genotypes. Stable incorporations of T-DNA into the citrus genome have been confirmed with both histochemical staining of GUS activity and molecular analyses. The majority of kn1 over-expressing citrus plants grow and develop normally at young seedling stages, similar to those of the wild type plants. With all six genotypes of citrus tested including Eureka lemon, a cultivar difficult to be transformed, our results demonstrate that the kn1 gene may provide an effective molecular tool to enhance genetic transformation efficiencies of various citrus varieties. High transformation efficiency of citrus is of great importance for large scale characterization of gene functions and also cultivar development via transgenic and genome editing technologies.

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Section: Introductionmentioning

confidence: 99%

Kn1 gene overexpression drastically improves genetic transformation efficiencies of citrus cultivars

Xie

et al. 2016

Plant Cell Tiss Organ Cult

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“…The transformation selection procedure should be improved to increase public acceptance, possibly by replacing traditional selection markers (nptII) with other markers (gfp, lecI, ipt, pmi, xyla), although the percentage of escapes is quite high (>40%) in fruit crops (May et al, 1995;Pena et al, 1995Pena et al, , 1997Mourgues et al, 1998;Perl and Eshdat, 1998). Endo et al (2001) suggested that the ipt gene from A. tumefaciens might be a suitable selectable gene. A multi-auto-transformation (MAT) vector, which combines the use of genes that stimulate growth and morphogenesis for positive selection of transformed cells with an excision mechanism to remove the markers and allow recovery of plants with normal phenotypes, could be very useful.…”

Section: Expected Technologiesmentioning

confidence: 99%

Olive

Rugini

Gutiérrez-Pesce

Muleo

2008

Compendium of Transgenic Crop Plants

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Biochemical and molecular procedures have been developed for olive genotyping, for taxonomy between cultivated and wild olives, for phylogenetic studies of cultivars, and for identification of usable markers linked to the most important agronomic traits, such as size of the tree, flowering induction, apical dominance, productivity, self‐fertility, quantity and quality of oil and secondary products of fruit, biotic and abiotic resistance. These characters could be introgressed into cultivars by conventional breeding or through transgeny. Successful results by classical breeding are difficult to obtain due to the different flower fertility and the long juvenile period of the progenies. The low efficiency of modern genetic improvement techniques suggests that genetic transformation techniques could speed up the development of new genotypes; however, the development of efficient regeneration methods from mature tissue is the limiting factor for applying this technology, in olive. Transgenic olive plants with modified growth habit and putative induced disease resistance are under filed conditions to test their phenotype. Other attempts of genetic improvement such as clonal selection, mutation through γ‐irradiation, protoplast technology, haploid, changes in ploidy level, somaclonal variation, and somatic hybridization are discussed and reviewed.

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“…Several positive selection systems have been developed in recent years. These include systems based on nonmetabolizable agents such as xylulose (Haldrup et al 1998), mannose (Joersbo et al 1998;Negrotto et al 2000;Reed et al 2001), 2-deoxyglucose (Kunze et al 2001), benzyladenine-N-3-glucuronide (Joersbo and Okkels 1996) or based on the promotion of plant regeneration without the use of a selective agent, such as isopentenyl transferase (Endo et al 2001;Zuo et al 2002). In previous reports of Oncidium transgenic plant selection utilizing the sweet pepper ferredoxin-like protein (pflp) gene and Erwinia carotovora as the selection agent .…”

Section: Introductionmentioning

confidence: 99%

Phosphomannose-isomerase as a selectable marker to recover transgenic orchid plants (Oncidium Gower Ramsey)

Thiruvengadam

Hsu

Yang

2010

Plant Cell Tiss Organ Cult

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A transformation method using the phosphomannose-isomerase (pmi) gene as a selectable marker was developed for orchid Oncidium Gower Ramsey. The pmigene, which converts mannose-6-phosphate to fructose-6-phosphate allowing for selection of transgenic plants on mannose selective medium. Genetically transformed plants of Oncidium were regenerated after cocultivating protocorm-like bodies with Agrobacterium tumefaciens strain GV3101 containing the vectors pEPYON-42P and pEP-YON-42H with 35S::PMI and 35S::HPTII genes respectively. We observed that 35S::PMI (pEPYON-42P) produced high rate (27 plants) of mannose resistant transgenic plants compared to 35S::HPTII (pEPYON-42H) in which only fourteen hygromycin resistant transgenic plants were obtained. Mannose resistant transgenic plants were confirmed by PCR and Southern blot. The pmi gene expression in 35S::PMI (pEPYON-42P) transgenic plants was confirmed by RT-PCR. Furthermore, the duration of regeneration time of transgenic plants was significantly shorter in mannose selected system (4 months) than in hygromycin selected system (8 months). The pmi/mannose selection system is shown to be highly efficient for producing transgenic O. Gower Ramsey without using antibiotics or herbicides. For the first time, the pmi/ mannose-based ''positive'' selection system has been used to obtain genetically engineered O. Gower Ramsey.

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The isopentenyl transferase gene is effective as a selectable marker gene for plant transformation in tobacco ( Nicotiana tabacum cv. Petite Havana SRI)

Cited by 50 publications

References 12 publications

Kn1 gene overexpression drastically improves genetic transformation efficiencies of citrus cultivars

Kn1 gene overexpression drastically improves genetic transformation efficiencies of citrus cultivars

Olive

Phosphomannose-isomerase as a selectable marker to recover transgenic orchid plants (Oncidium Gower Ramsey)

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