The issue of HPLC determination of endogenous lipoic acid in human plasma

Sechovcová, Soňa; Královcová, P.; Kanďár, Roman; Ventura, Karel

doi:10.1002/bmc.4172

Cited by 5 publications

(5 citation statements)

References 8 publications

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“…HPLC-CD has been successfully applied to isoflavonoids and lignans [87], a wide range of other phenolic [88][89][90][91][92][93][94][95][96][97][98], lipophilic [89,99,100] and S-containing AOs [101][102][103][104][105] (Table 4). The limits of detection achieved are impressive (up to the fmol level).…”

Section: Coulometric Detection Of Antioxidants In Chromatography and Flow Systemsmentioning

confidence: 99%

Analytical Capabilities of Coulometric Sensor Systems in the Antioxidants Analysis

Ziyatdinova

Budnikov

2021

Chemosensors

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The definition of antioxidants (AOs), their classification and properties as well as electrochemical sensor systems for AOs analysis are briefly discussed. The analytical capabilities of coulometric titration with electrogenerated titrants as sensor systems for AOs determination have been considered in detail. The attention focused on the individual AO quantification that was mainly used in the pharmaceutical analysis and estimation of total antioxidant parameters (total antioxidant capacity (TAC), ferric reducing power (FRP) and ceric reducing/antioxidant capacity (CRAC)) allowing the fast screening of the target samples including their quality control. The main advantages of coulometric sensor systems are pointed out. The selective quantification of individual AO in a complex matrix using a combination of chromatography with coulometric or coulometric array detection under potentiostatic mode is discussed. The future development of coulometric sensor systems for AOs analysis is focused on the application of novel coulometric titrants and the application of coulometric detection in flow injection analysis.

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Section: Coulometric Detection Of Antioxidants In Chromatography and Flow Systemsmentioning

confidence: 99%

Analytical Capabilities of Coulometric Sensor Systems in the Antioxidants Analysis

Ziyatdinova

Budnikov

2021

Chemosensors

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“…Sechovcová et al (49) have reported that endogenous plasma levels of lipoic acid range between <4.9 and 197.0 nmol/l, determined using a conventional method. The authors developed a new method using high-performance liquid chromatography to determine endogenous lipoic acid levels.…”

Section: Discussionmentioning

confidence: 99%

α‑lipoic acid inhibits cerulein/resistin‑induced expression of interleukin‑6 by activating peroxisome proliferator‑activated receptor‑γ in pancreatic acinar cells

2022

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cerulein-induced pancreatitis resembles human acute pancreatitis in terms of pathological events, such as enzymatic activation and inflammatory cell infiltration in the pancreas. cerulein is a cholecystokinin analog that increases levels of reactive oxygen species (roS) and interleukin-6 (il-6) expression level in pancreatic acinar cells. Serum levels of resistin, which is secreted from adipocytes, are reportedly higher in patients with acute pancreatitis than in healthy individuals. Previously, it was shown that the adipokine resistin can aggravate the cerulein-induced increase in roS levels and il-6 expression level in pancreatic acinar cells. Peroxisome proliferator-activated receptor-gamma (PPar-γ) is a key regulator of the transcription and expression of antioxidant enzymes, including heme oxygenase 1 (Ho-1) and catalase. α-lipoic acid, a naturally occurring dithiol antioxidant, can prevent cerulein-induced pancreatic damage in rats. in the present study, it was aimed to investigate whether α-lipoic acid can attenuate the cerulein/resistin-induced increase in il-6 expression and roS levels via PPar-γ activation in pancreatic acinar AR42J cells. The anti-inflammatory mechanism of α-lipoic acid was determined using reverse transcription-quantitative Pcr, western blot analysis, enzyme-linked immunosorbent assay, immunofluoresc2ence staining and fluorometry. Treatment with cerulein and resistin increased roS levels and il-6 expression level, which were inhibited by α-lipoic acid in pancreatic acinar cells. α-lipoic acid increased the nuclear translocation and expression level of PPar-γ and the expression levels of its target genes: Ho-1 and catalase. The PPar-γ antagonist GW9662 and Ho-1 inhibitor zinc protoporphyrin reversed the inhibitory effect of α-lipoic acid on cerulein/resistin-induced increase in roS and il-6 levels. in conclusion, α-lipoic acid inhibits the cerulein/resistin-induced increase in roS production and il-6 expression levels by activating PPar-γ and inducing the expression of Ho-1 and catalase in pancreatic acinar cells.

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“… Typical chromatograms with electrochemical detection of sulfur-containing antioxidants: ( a ) Chromatograms of plasma without addition of lipoic acid ( 1 ) and with additions of 1 ( 2 ), 5 ( 3 ), 7.5 ( 4 ), 15 ( 5 ) and 50 nM ( 6 ) of lipoic acid. Reprinted from [ 169 ], copyright 2017, with permission from John Wiley & Sons, Ltd. ( b ) Schematic illustration of the simultaneous determination of cysteine (Cys), homocysteine (hCys), glutathione (GSH), and methionine (Met) using green-synthesized Au nanoparticles/Au SPE. Reprinted from [ 177 ], copyright 2019, with permission from Elsevier.…”

Section: Figures Schemes and Tablesmentioning

confidence: 99%

Recent Advances in Electrochemical Sensors for Sulfur-Containing Antioxidants

Ziyatdinova

Gimadutdinova

2023

Micromachines

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Sulfur-containing antioxidants are an important part of the antioxidant defense systems in living organisms under the frame of a thiol–disulfide equilibrium. Among them, l-cysteine, l-homocysteine, l-methionine, glutathione, and α-lipoic acid are the most typical representatives. Their actions in living systems are briefly discussed. Being electroactive, sulfur-containing antioxidants are interesting analytes to be determined using various types of electrochemical sensors. Attention is paid to the chemically modified electrodes with various nanostructured coverages. The analytical capabilities of electrochemical sensors for sulfur-containing antioxidant quantification are summarized and discussed. The data are summarized and presented on the basis of the electrode surface modifier applied, i.e., carbon nanomaterials, metal and metal oxide nanoparticles (NPs) and nanostructures, organic mediators, polymeric coverage, and mixed modifiers. The combination of various types of nanomaterials provides a wider linear dynamic range, lower limits of detection, and higher selectivity in comparison to bare electrodes and sensors based on the one type of surface modifier. The perspective of the combination of chromatography with electrochemical detection providing the possibility for simultaneous determination of sulfur-containing antioxidants in a complex matrix has also been discussed.

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The issue of HPLC determination of endogenous lipoic acid in human plasma

Cited by 5 publications

References 8 publications

Analytical Capabilities of Coulometric Sensor Systems in the Antioxidants Analysis

Analytical Capabilities of Coulometric Sensor Systems in the Antioxidants Analysis

α‑lipoic acid inhibits cerulein/resistin‑induced expression of interleukin‑6 by activating peroxisome proliferator‑activated receptor‑γ in pancreatic acinar cells

Recent Advances in Electrochemical Sensors for Sulfur-Containing Antioxidants

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