The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting

Balaban, Başak; Brison, Daniel R.; Calderón, Gloria; Catt, James; Conaghan, J.; Cowan, Lisa; Ebner, Thomas; Gardner, David K.; Hardarson, Thorir; Lundin, Kersti; Magli, M. Cristina; Mortimer, David; Mortimer, Sharon T.; Munné, Santiago; Royère, Dominique; Scott, Lynette; Smitz, Johan; Thornhill, Alan R.; Blerkom, Jonathan Van; Abbeel, E. Van den

doi:10.1093/humrep/der037

Cited by 1,465 publications

(561 citation statements)

References 74 publications

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“…Apart from a more detailed and flexible embryo evaluation, these studies suggest that time-lapse monitoring may provide new dynamic markers of embryo competence. There is a well-documented correlation between embryo morphology evaluated at certain time-points and embryo competence as reviewed in a recent consensus paper by ALPHA and ESHRE [16]. However, the progressive and dynamic nature of cell cleavage and embryo development is also well known [13,18,30], and embryo scoring can change markedly within few hours [25].…”

Section: Discussionmentioning

confidence: 99%

A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

Kirkegaard

Hindkjær

Grøndahl

et al. 2012

J Assist Reprod Genet

130

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Purpose Time-lapse monitoring allows for a flexible embryo evaluation and potentially provides new dynamic markers of embryo competence. Before introducing timelapse monitoring in a clinical setting, the safety of the instrument must be properly documented. Accordingly, the aim of this study was to evaluate the safety of a commercially available time-lapse incubator. Methods In a two center, randomized, controlled, clinical trial 676 oocytes from 59 patients in their 2nd or third treatment cycle, age <38 years and ≥8 oocytes retrieved were cultured in the time-lapse incubator or in a conventional incubator. The primary outcome was proportion of 4-cell embryos on day 2. Secondary outcomes were proportion of 7-8 cell embryos on day 3 and proportion of blastocysts on day 5. Implantation pregnancy rates were registered based on presence of fetal heart activity visualized by ultrasound 8 weeks after embryo transfer. Results No significant difference was found between the time-lapse incubator (TLI) and conventional incubator (COI) in proportion of 4-cell embryos on day 2 irrespective of whether data was analyzed according to ITT (RR TLI/COI : 0.81 (0.65; 1.02)) or PP (RR TLI/COI : 0.80 (0.63; 1.01)). Nor were any significant differences detected in the secondary endpoints; i.e. proportion of 7-8-cell embryos on day three ITT (RR TLI/COI : 0.96 (0.73; 1.26)); PP (RR TLI/COI : 0.95 (0.72; 1.26)) and proportion of blastocysts on day five ITT (RR TLI/COI : 1.09 (0.84; 1.41)); PP (RR TLI/COI : 1.09 (0.83: 1.41)). We found no differences in clinical pregnancy rate or implantation rate. Conclusion Culture in the time-lapse incubator supports embryonic development equally to a conventional incubator.

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Section: Discussionmentioning

confidence: 99%

A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

Kirkegaard

Hindkjær

Grøndahl

et al. 2012

J Assist Reprod Genet

130

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“…Based on the analysis of time-lapse records, and in accordance with the guidelines of the American Society for Reproductive Medicine and the European Society of Human Reproduction and Embryology [23], a single blastocyst was selected to be transferred into the uterus.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of trace metals in follicular fluid in ICSI-treated patients

Wdowiak¹,

Wdowiak²,

Bojar³

2018

Ann Agric Environ Med.

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This study investigated the influence of cadmium (Cd), copper (Cu), iron (Fe), lead (Pb), selenium (Se), and zinc (Zn) in follicular fluid on the effectiveness of in vitro fertilization (IVF) treatment and the dynamics of embryo development. The study involved 221 women aged 25-35 years in whom intracytoplasmic sperm injection (ICSI) was performed. Analysis of the effects of the average concentrations of Se, Zn, and Cu on the developmental dynamics of embryos showed that higher concentrations of these metals were accompanied by shorter lead times for each of the various stages of development, while the reverse was true in the case of cadmium and lead. No relationship was observed between the mean concentrations of Fe and the dynamics of the human embryo. In order to discover whether the fact of becoming pregnant is affected by the investigated variables, a logistic regression model was applied. The dependent variable was the fact of achieving pregnancy, and the independent variable tested was the level of trace metals. Ultimately, only one variable remained statistically significant in the model: the level of Se.

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“…During the culture, each embryo is observed and graded once a day; the grade is assigned by assessing the morphology of the embryo according to state of the art criteria [22,3,2]. Embryos whose morphology meets several criteria are graded as top, namely they are expected to have higher probability of implantation (if transferred into a receptive uterus) than non-top ones.…”

Section: Scoring Embryosmentioning

confidence: 99%

“…Embryos are cultured for 2-5 days, before being transferred to the woman. During the culture, the morphology of each embryo is monitored at fixed time intervals; embryos with certain morphologies have indeed high implantation potential [1,2,3] and are thus graded as of top quality. Despite the effort for designing effective scoring system for the embryos [2], predicting blastocyst development remains a challenging problem [4], although promising results have been recently obtained by analyzing time-lapse embryo images collected by automated image monitoring systems [5,6].…”

Section: Introductionmentioning

confidence: 99%

A Bayesian network model for predicting pregnancy after in vitro fertilization

Corani

Magli²,

Giusti

et al. 2013

Computers in Biology and Medicine

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We present a Bayesian network model for predicting the outcome of in vitro fertilization (IVF). The problem is characterized by a particular missingness process; we propose a simple but effective averaging approach which improves parameter estimates compared to the traditional MAP estimation. We present results with generated data and the analysis of a real data set. Moreover, we assess by means of a simulation study the effectiveness of the model in supporting the selection of the embryos to be transferred.

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The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting

Cited by 1,465 publications

References 74 publications

A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

Evaluation of trace metals in follicular fluid in ICSI-treated patients

A Bayesian network model for predicting pregnancy after in vitro fertilization

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