The iteration method of data censoring in the regression estimation problem

Kirik, Ekaterina

doi:10.1134/s000511790704008x

Cited by 1 publication

(2 citation statements)

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“…Only proteins with two or more peptides were considered significant for further analysis. Grubbs test for outliers 27 was used to remove statistical outliers before calculating a mean value of normalized fold values. Proteins with ratio ±1×SD were considered as up and downregulated.…”

Section: Methodsmentioning

confidence: 99%

“…Ratios were calculated by dividing heavy by light (H/L)/ median. Significance level for up or downregulation was calculated by 1× SD after removal of outliers by Grubb's test 27, http://www.probe.uib.no/forum/index.php?topic=68.msg84#msg84. Median values for experiment 1 were 0.603 for both cytosol and nuclear fractions and for experiment 2: 0.948 and 0.7 for cytosolic and nuclear fractions, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Increased interaction between DJ‐1 and the Mi‐2/ nucleosome remodelling and deacetylase complex during cellular stress

et al. 2010

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DJ-1 was originally identified to be an oncogenic product, but has later been shown to be highly multifunctional. DJ-1 plays a role in oxidative stress response and transcriptional regulation, and loss of its function leads to an early onset of Parkinsonism. To further understand the mechanisms behind DJ-1's role in cell survival and death, we investigated alternations in endogenous DJ-1 protein-protein interaction in apoptotic cells exposed to the phosphatase inhibitor okadaic acid. By combining cellular stable isotopic labelling of amino acids in cell culture, sub-cellular fractionation, co-immunoprecipitation, and MS, we identified a novel group of DJ-1 interaction partners that increased their association to DJ-1 in okadaic acid-exposed cells. These proteins were integral components of the Mi-2/nucleosome remodelling and deacetylase (NuRD) complex. Knockdown of DJ-1 and MTA2, a core component of the NuRD complex, had a similar and pro-apoptotic effect on the transcriptional- and p53-dependent cell death induced by daunorubicin. On the other hand, MTA2 knockdown had no significant effect on the progression of p53-independent okadaic acid-induced apoptosis. Our data suggest that the increased DJ-1/NuRD interaction is a general anti-stress response regulated by okadaic acid-induced modifications of DJ-1. The observed interaction between DJ-1 and the NuRD complex may give new clues to how DJ-1 can protect cells from p53-dependent cell death.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%