The Jet Stream Microbeveler: An Inexpensive Way to Bevel Ultrafine Glass Micropipettes

Ogden, Thomas E.; Citron, Mark C.; Pierantoni, Ruggero

doi:10.1126/science.663670

Cited by 87 publications

(29 citation statements)

References 9 publications

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“…They were filled with 4% HRP in 0.5 M KC l and 0.04 M Tris buffer, pH 8.6. A jet stream beveler (Ogden et al, 1978) was used to lower the resistance of the electrode from 200 -400 to 30 -100 M⍀. An Ag-AgC l wire was used to couple the microelectrode to a high-impedance DC amplifier.…”

Section: Methodsmentioning

confidence: 99%

Intracellular Recordings in Response to Monaural and Binaural Stimulation of Neurons in the Inferior Colliculus of the Cat

et al. 1997

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The inferior colliculus (IC) is a major auditory structure that integrates synaptic inputs from ascending, descending, and intrinsic sources. Intracellular recording in situ allows direct examination of synaptic inputs to the IC in response to acoustic stimulation. Using this technique and monaural or binaural stimulation, responses in the IC that reflect input from a lower center can be distinguished from responses that reflect synaptic integration within the IC. Our results indicate that many IC neurons receive synaptic inputs from multiple sources. Few, if any, IC neurons acted as simple relay cells. Responses often displayed complex interactions between excitatory and inhibitory sources, such that different synaptic mechanisms could underlie similar response patterns. Thus, it may be an oversimplification to classify the responses of IC neurons as simply excitatory or inhibitory, as is done in many studies. In addition, inhibition and intrinsic membrane properties appeared to play key roles in creating de novo temporal response patterns in the IC.

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Section: Methodsmentioning

confidence: 99%

Intracellular Recordings in Response to Monaural and Binaural Stimulation of Neurons in the Inferior Colliculus of the Cat

et al. 1997

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“…The tip resistance of these microelectrodes usually ranged between 20 and 30 megohms, and in many experiments the resistance was reduced to a range between 10 and 15 megohms by bevelltng according to methods described previously (Ogden et al, 1978). The current electrode was positioned near the middle of the muscle fiber and the voltage electrode was about 50 to 100 wrn apart from it.…”

Section: Methodsmentioning

confidence: 99%

Voltage clamp analysis of membrane currents in larval muscle fibers of Drosophila: alteration of potassium currents in Shaker mutants

Wu¹,

Haugland²

1985

J. Neurosci.

180

168

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The body wall muscles in Drosophila larvae are suitable for voltage clamp analysis of changes in membrane excitability caused by mutations.Both inward and outward ionic currents are present in these muscle fibers. The inward current is mediated by voltage-dependent Ca*+ channels. In Ca2+-free saline, the inward current is eliminated.The remaining outward K+ currents consist of two distinct components, an early transient IA and a delayed steady IK, which are separable by differences in the rate and voltage dependence of activation and inactivation. The steady-state and kinetic properties of the activation and inactivation processes of these two currents are analyzed.

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“…Electrodes with resistances between 100 and 200 MQ were used. For dye-injection, electrodes were filled with a 4% horseradish peroxidase (HRP) (Boehringer, Grade 1) in 0 5 Mpotassium acetate solution and bevelled down to about 350 MQ using a modified jet-stream microbeveller (Ogden, Citron & Pierantoni, 1978). Penetrations were made by mounting the micro-electrode on a piezoelectric 'prodder' (Weevers, 1980) in a manipulator with hydraulic advance.…”

Section: Methodsmentioning

confidence: 99%

Sensory physiology, anatomy and immunohistochemistry of Rohon‐Beard neurones in embryos of Xenopus laevis.

Clarke¹,

Hayes²,

Hunt³

et al. 1984

The Journal of Physiology

169

121

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SUMMARY1. Rohon-Beard neurones show substance P-like immunoactivity in their somas and in their centrally projecting axons. Peripherally, the morphology of their free nerve endings within the trunk skin has been shown using horseradish peroxidase staining.2. The excitation of Rohon-Beard neurones by natural and electrical stimulation of the skin has been examined using intracellular micro-electrodes in the late embryo of Xenopus laevis.3. Rohon-Beard cells are sensitive to transient, local indentation of the trunk skin, responding with one or a few impulses. They adapt rapidly to repeated stimulation. They can also be excited by a brief current pulse to the skin. They are not sensitive to slow indentation of the skin, nor are they excited by epithelial action potentials.4. The responses to skin stimulation are not abolished by a Ringer solution containing 12 mM-Mg2+ and only 05 mM-Ca2+.5. Intracellularly evoked action potentials in single Rohon-Beard cells are sometimes sufficient to evoke sustained episodes of fictive swimming.6. The results indicate that Rohon-Beard cells are responsible for detecting light touch stimuli to the embryo's body and for initiating swimming in response to this stimulus.

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The Jet Stream Microbeveler: An Inexpensive Way to Bevel Ultrafine Glass Micropipettes

Cited by 87 publications

References 9 publications

Intracellular Recordings in Response to Monaural and Binaural Stimulation of Neurons in the Inferior Colliculus of the Cat

Intracellular Recordings in Response to Monaural and Binaural Stimulation of Neurons in the Inferior Colliculus of the Cat

Voltage clamp analysis of membrane currents in larval muscle fibers of Drosophila: alteration of potassium currents in Shaker mutants

Sensory physiology, anatomy and immunohistochemistry of Rohon‐Beard neurones in embryos of Xenopus laevis.

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