The junctional epithelium originates from the odontogenic epithelium of an erupted tooth

Yajima-Himuro, Sara; Oshima, Masamitsu; Yamamoto, Gou; Ogawa, Miho; Furuya, Madoka; Tanaka, Junichi; Nishii, K; Mishima, Kenji; Tachikawa, Tetsuhiko; Tsuji, Takashi; Yamamoto, Masahide

doi:10.1038/srep04867

Cited by 41 publications

(26 citation statements)

References 44 publications

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“…However, there was no obvious difference in the morphology of JE among the three groups after wound healing; the fast ability of JE to self-renew may be the most likely explanation for our results. 43 , 44 The self-renewal time of the normal JE is 1–6 days. Therefore, it is difficult to distinguish them morphologically.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, our data revealed that the colocalization band, indicating HDs first formed in the coronal region of the JE, since the cells directly attached to the tooth, migrates and adheres toward the crown. 44 To verify the efficacy of epithelial reattachment, we adopted the HRP-penetration assay to assess the biological sealing of the tooth–JE interface. 49 From the penetration distance of the HRP reaction, the Ad-LAMA3 group exhibited higher biological sealing capacity than the saline and Ad-GFP groups.…”

Section: Discussionmentioning

confidence: 99%

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Adenovirus-Mediated LAMA3 Transduction Enhances Hemidesmosome Formation and Periodontal Reattachment during Wound Healing

Zhang

Zhen-xuan

et al. 2020

Molecular Therapy - Methods & Clinical Development

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A robust dento-epithelial junction prevents external pathogenic factors from entering connective tissue and could be crucial for periodontal reattachment after periodontal surgery. The junctional epithelium (JE) is attached to the tooth surface through the hemidesmosome (HD) and internal basal lamina, where the primary component is laminin-332. Destruction of the JE leads to the loss of periodontal attachment. Traditional treatments are effective in eliminating local inflammation of the gingiva; however, few directly promote periodontal reattachment and HD formation. Here, we designed a gene-therapy strategy using the adenovirus-mediated human laminin-332 α3 chain (LAMA3) gene (Ad-LAMA3) transduced into a human-immortalized epidermal cell line (HaCaT) to study the formation of HD in vitro . Ad-LAMA3 promoted early adhesion and fast migration of HaCaT cells and increased expression of LAMA3 and type XVII collagen (BP180) significantly. Furthermore, HaCaT cells could facilitate formation of mature HDs after LAMA3 overexpression. In vivo experiments demonstrated that the JE transduced with Ad-LAMA3 could increase expression of LAMA3 and BP180 and “biological sealing” between the tooth and gingival epithelium. These results suggested that adenovirus-mediated LAMA3 transduction is a novel therapeutic strategy that promotes the stability and integration of the JE around the tooth during wound healing.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Adenovirus-Mediated LAMA3 Transduction Enhances Hemidesmosome Formation and Periodontal Reattachment during Wound Healing

Zhang

Zhen-xuan

et al. 2020

Molecular Therapy - Methods & Clinical Development

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“…JE is a special tissue of odontogenic origin which includes post‐functional ameloblasts of the epithelium of reduced the enamel organ . Once the formation of enamel matrix is concluded, these cells turn into reduced ameloblasts that become part of the reduced enamel epithelium . This epithelium wraps the crown, and when the tooth is about to erupt, unites with the basal cells of the gingival epithelium allowing the tooth eruption and creating the final mature JE …”

Section: Discussionmentioning

confidence: 99%

Characterization of juvenile spongiotic gingival hyperplasia as an entity of odontogenic origin

Mendoza

Alberdi-Navarro

Marichalar-Mendía

et al. 2019

Journal of Periodontology

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Background Juvenile spongiotic gingival hyperplasia (JSGH) is a distinct clinicopathological entity of the buccal gingiva of young patients which has been related to several factors such as plaque formation, hormonal modifications, and viral infections; however, its true etiopathogenesis remains unsolved. Several immunohistochemical studies have demonstrated the similarity between the junctional epithelium (JE) and the hyperplasic epithelium of JSGH. The objective of this study is to analyze the clinicopathological and immunohistochemical characteristics of JSGH to explain its origin. Methods Clinicopathlogical data of 10 cases of JSGH (five men and five women) with a mean age of 13 years (range: 9 to 17 years) were collected. CK7, CK14, CK19, CD3, CD20, S100, and Ki‐67 antibodies were used for comparative immunohistochemical study. Results All the lesions showed hyperplasic epithelium in its central portion, exhibiting marked spongiosis, vascular proliferation, and a chronic inflammatory infiltrate on the subepithelial connective tissue. CK19 was positive in the whole hyperplasic epithelium of JSGH and the basal layer of the marginal gingiva, while expression of CK14 was present in all epithelial layers of both the JSGH and that of the marginal gingiva. The subepithelial inflammatory infiltrate has a larger amount of CD20 positive cells. Conclusion JSGH is a reactive tumor of the gingiva that may have an odontogenic etiology, whose origin seems to be the remnants of JE.

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“…Furthermore, epithelial cells from mouse embryos have been showed to result in the tooth like frameworks if joined with embryonic dental mesenchyme, however, not whole functional teeth [42][43][44]. Yajima-Himuro et al [45] have transplanted bioengineered tooth germ into the alveolar bone with an epithelial component, and concluded that epithelium from the bioengineered tooth fused with the oral epithelium, and the junctional epithelium was apparently formed around the bioengineered tooth 50 days after transplantation.…”

Section: Provenance Of Dental Stem Cellsmentioning

confidence: 99%

Future perspective of new tooth creation

Kheirallah

2017

Oral Biol Res

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Currently, outcomes of dental stem cells studies indicate that the tooth is an available source for clinical application. Finding a universal cell that can morph into many several types of oral tissues is a key for new tooth creation. If dental stem cells are isolated to form new tooth buds, with creation of an imitative condition to the embryonic jaw, this will be a revolutionary new way to replace a missing tooth. Although investigators have created tooth-like structures in studies, so far, later dental stem cells investigations will be able to create a workable vascularized implant in the human body. This review study discusses current challenges in creating a biological environment that mimics the condition of teeth as they form in an embryo.

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The junctional epithelium originates from the odontogenic epithelium of an erupted tooth

Cited by 41 publications

References 44 publications

Adenovirus-Mediated LAMA3 Transduction Enhances Hemidesmosome Formation and Periodontal Reattachment during Wound Healing

Adenovirus-Mediated LAMA3 Transduction Enhances Hemidesmosome Formation and Periodontal Reattachment during Wound Healing

Characterization of juvenile spongiotic gingival hyperplasia as an entity of odontogenic origin

Future perspective of new tooth creation

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