The juvenile hormone epoxide hydrolase homolog in Penaeus vannamei plays immune-related functions

Liu, Zhuoyan; Huang, Zishu; Zheng, Xiaoyu; Zheng, Zhihong; Yao, Defu; Zhang, Yueling; Aweya, Jude Juventus

doi:10.1016/j.dci.2022.104410

Cited by 9 publications

(7 citation statements)

References 54 publications

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“…In crustaceans, the JHEH-like genes have been identified in Daphnia magna (Toyota et al, 2014), M. rosenbergii (Chen et al, 2021) and Penaeus vannamei (Liu et al, 2022). JHEH-like is indispensable for the regulation of larval development and ecdysteroidogenesis in M. rosenbergii (Chen et al, 2021) and plays an essential role in shrimp survival during bacterial infection in P. vannamei (Liu et al, 2022). In this study, the expression of JHEH-like was significantly highest in the major growth stage among the five ovarian developmental stages in E. carinicauda.…”

Section: ) Have Multiple Vitellogenin Genes Previous Studies Onmentioning

confidence: 56%

“…Knocking down JHEH in Colaphellus bowringi elevated JH signaling and increased expressions of vitellogenin genes (Guo et al, 2019b). In crustaceans, the JHEH-like genes have been identified in Daphnia magna (Toyota et al, 2014), M. rosenbergii (Chen et al, 2021) and Penaeus vannamei (Liu et al, 2022). JHEH-like is indispensable for the regulation of larval development and ecdysteroidogenesis in M. rosenbergii (Chen et al, 2021) and plays an essential role in shrimp survival during bacterial infection in P. vannamei (Liu et al, 2022).…”

Section: ) Have Multiple Vitellogenin Genes Previous Studies Onmentioning

confidence: 99%

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Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analysis Provide Insights Into the Ovarian Maturation of Exopalaemon carinicauda

Wang

Li²,

Ge³

et al. 2022

Front. Mar. Sci.

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The ridgetail white shrimp Exopalaemon carinicauda has the potential to be used as a useful experimental organism in the field of crustacean research due to its transparent body, large egg, and short reproductive cycle. However, little is known about the regulatory mechanisms of ovarian maturation in E. carinicauda. In this study, we applied PacBio single-molecule long-read sequencing technology to unveil the whole transcriptome landscape of Exopalaemon carinicauda to better understand the molecular mechanisms of ovarian development. A total of 49.05 G polymerase read bases were generated, finally, 15, 151 unigenes were successfully identified with a mean length of 4, 257 bp and an N50 of 4, 884 bp from ovary tissues (five different ovarian developmental stages). A total of 14, 007 unigenes were successfully annotated in at least one public database. In addition, 8, 861 coding sequences, 4, 594 lncRNAs and 895 transcription factors were identified. Furthermore, we performed RNA-Seq analysis combined with Iso-Seq results to investigate ovarian maturation regulation mechanism and validated the results by quantitative real-time PCR. We annotated five vitellogenin isoforms in the full-length transcriptome, meanwhile these five genes and vitellogenin receptor suggested an important role during previtellogenesis of E. carinicauda. Genes were involved in PI3K-Akt signaling pathway, retinol metabolism, cell cycle and hedgehog signaling pathway, which showed obvious associations with ovarian maturation of E. carinicauda. We identified 40 DEGs, such as forkhead box protein L2, which may be related to ovarian development in the comparisons. The expression profiles of genes such as ecdysone receptor, ecdysone-induced protein 74EF, ecdysone inducible gene E75 and post-molt protein suggest that they were significantly associated with reproductive molting in the ovarian mature stage. In conclusion, the present study identified important genes and pathways involved in ovarian maturation, which might be useful for studying the reproductive regulation and mechanisms of ovarian maturation in E. carinicauda.

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Section: ) Have Multiple Vitellogenin Genes Previous Studies Onmentioning

confidence: 56%

Section: ) Have Multiple Vitellogenin Genes Previous Studies Onmentioning

confidence: 99%

Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analysis Provide Insights Into the Ovarian Maturation of Exopalaemon carinicauda

Wang

Li²,

Ge³

et al. 2022

Front. Mar. Sci.

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“…It was previously supposed that epoxidized JHs did not exist in crustacean species. However, the existence of a CYP15-like gene [ 5 , 42 , 43 ], which catalyzes the epoxidation of MF [ 3 ], and juvenile hormone epoxide hydrolase (JHEH) [ 44 ], which catalyzes the epoxide hydration of JHs, suggested that the epoxidized JHs may also existed in crustacean species. To make a further prediction, a phylogenetic tree using the protein sequences of the CYP15-like gene family was also constructed ( Supplementary Figure S3 ).…”

Section: Resultsmentioning

confidence: 99%

Identification and Evolution Analysis of the Complete Methyl Farnesoate Biosynthesis and Related Pathway Genes in the Mud Crab, Scylla paramamosain

Zhao

Zhang

Wang

et al. 2022

IJMS

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The sesquiterpenoid hormone methyl farnesoate (MF) plays a vital role during crustacean development, which is mainly evidenced by its varied titers during different developmental stages. However, the biosynthesis pathways of MF remain obscure to some extent. In this study, we identified the complete MF biosynthesis and related pathway genes in Scylla paramamosain, including three involved in acetyl-CoA metabolism, eight in the mevalonate pathway, five in the sesquiterpenoids synthesis pathway, and five in the methionine cycle pathway. Bioinformatics, genomic structure, and phylogenetic analysis indicated that the JH biosynthesis genes might have experienced evolution after species differentiation. The mRNA tissue distribution analysis revealed that almost all genes involving in or relating to MF syntheses were highly expressed in the mandibular organ (MO), among which juvenile hormone acid methyltransferase was exclusively expressed in the MO, suggesting that most of these genes might mainly function in MF biosynthesis and that the methionine cycle pathway genes might play a crucial regulatory role during MF synthesis. In addition, the phylogenetic and tissue distribution analysis of the cytochrome P450 CYP15-like gene suggested that the epoxidized JHs might exist in crustaceans, but are mainly synthesized in hepatopancreas rather than the MO. Finally, we also found that betaine-homocysteine S-methyltransferase genes were lost in insects while methionine synthase was probably lost in most insects except Folsomia candida, indicating a regulatory discrepancy in the methionine cycle between crustaceans and insects. This study might increase our understanding of synthetic metabolism tailored for sesquiterpenoid hormones in S. paramamosain and other closely related species.

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“…This observation is consistent with a previous study where the expression of Kr-h1 gene in T. castaneum was significantly upregulated after Paranosema whitei infection ( 22 ). Moreover, our recent data revealed that juvenile hormone epoxide hydrolase (JHEH), an enzyme that breaks down juvenile hormone, which is also involved in the development and molting of insects, plays an essential role in shrimp survival during bacterial infection ( 36 ). Therefore, Kr-h1 is important in regulating key physiological processes in crustaceans and also plays an essential role in the innate immune response during AHPND in shrimp.…”

Section: Discussionmentioning

confidence: 99%

Kruppel homolog 1 modulates ROS production and antimicrobial peptides expression in shrimp hemocytes during infection by the Vibrio parahaemolyticus strain that causes AHPND

Zheng,

Liu,

Lin

et al. 2023

Front. Immunol.

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Shrimp aquaculture has been seriously affected by acute hepatopancreatic necrosis disease (AHPND), caused by a strain of Vibrio parahaemolyticus that carries the Pir toxin plasmids (V. parahaemolyticus(AHPND)). In this study, the transcription factor, Kruppel homolog 1-like of Peneaus vannamei (PvKr-h1), was significantly induced in shrimp hemocytes after V. parahaemolyticus(AHPND) challenge, suggesting that PvKr-h1 is involved in shrimp immune response. Knockdown of PvKr-h1 followed by V. parahaemolyticus(AHPND) challenge increased bacterial abundance in shrimp hemolymph coupled with high shrimp mortality. Moreover, transcriptome and immunofluorescence analyses revealed that PvKr-h1 silencing followed by V. parahaemolyticus(AHPND) challenge dysregulated the expression of several antioxidant-related enzyme genes, such as Cu-Zu SOD, GPX, and GST, and antimicrobial peptide genes, i.e., CRUs and PENs, and reduced ROS activity and nuclear translocation of Relish. These data reveal that PvKr-h1 regulates shrimps’ immune response to V. parahaemolyticus(AHPND) infection by suppressing antioxidant-related enzymes, enhancing ROS production and promoting nuclei import of PvRelish to stimulate antimicrobial peptide genes expression.

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The juvenile hormone epoxide hydrolase homolog in Penaeus vannamei plays immune-related functions

Cited by 9 publications

References 54 publications

Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analysis Provide Insights Into the Ovarian Maturation of Exopalaemon carinicauda

Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analysis Provide Insights Into the Ovarian Maturation of Exopalaemon carinicauda

Identification and Evolution Analysis of the Complete Methyl Farnesoate Biosynthesis and Related Pathway Genes in the Mud Crab, Scylla paramamosain

Kruppel homolog 1 modulates ROS production and antimicrobial peptides expression in shrimp hemocytes during infection by the Vibrio parahaemolyticus strain that causes AHPND

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