The K+ and NO3− Interaction Mediated by NITRATE TRANSPORTER1.1 Ensures Better Plant Growth under K+-Limiting Conditions

Fang, Xian Zhi; Liu, Xing Xing; Zhu, Ya Xing; Ye, Jiayuan; Jin, Chong

doi:10.1104/pp.20.01229

Cited by 23 publications

(20 citation statements)

References 71 publications

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“…It has been speculated that AtAKT1‐mediated K + uptake and AtCHL1‐mediated NO 3 − uptake may be coordinated through the AtCBL1/9–AtCIPK23 complex. A recent report confirmed that AtNRT1.1/AtCHL1 is coordinated with K + channels/transporters located in the root epidermis–cortex and central vasculature to improve K + uptake and root‐to‐shoot allocation, respectively (Fang et al, 2020).…”

Section: Interactions Among Nitrogen Potassium and Phosphorusmentioning

confidence: 86%

Potassium and phosphorus transport and signaling in plants

Wang

Chen

2021

JIPB

229

108

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Nitrogen (N), potassium (K), and phosphorus (P) are essential macronutrients for plant growth and development, and their availability affects crop yield. Compared with N, the relatively low availability of K and P in soils limits crop production and thus threatens food security and agricultural sustainability. Improvement of plant nutrient utilization efficiency provides a potential route to overcome the effects of K and P deficiencies. Investigation of the molecular mechanisms underlying how plants sense, absorb, transport, and use K and P is an important prerequisite to improve crop nutrient utilization efficiency. In this review, we summarize current understanding of K and P transport and signaling in plants, mainly taking Arabidopsis thaliana and rice (Oryza sativa) as examples. We also discuss the mechanisms coordinating transport of N and K, as well as P and N.

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Section: Interactions Among Nitrogen Potassium and Phosphorusmentioning

confidence: 86%

Potassium and phosphorus transport and signaling in plants

Wang

Chen

2021

JIPB

229

108

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“…NRT1.1 also functions as a symporter, transporting H + and nitrate [75]. In Arabidopsis , a reduction of Nrt1.1 expression leads to acidification of the growth medium [76]. Similarly, reduced nitrate transport under low N may free up protons to drive additional P transport…”

Section: Discussionmentioning

confidence: 99%

Low nitrogen availability inhibits the phosphorus starvation response in maize (Zea maysssp.maysL.)

Torres-Rodríguez

Salazar-Vidal

Montes

et al. 2020

Preprint

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Background: Nitrogen (N) and phosphorus (P) are macronutrients essential for crop growth and productivity. In cultivated fields, N and P levels are rarely sufficient, contributing to the yield gap between realized and potential production. Fertilizer application increases nutrient availability, but not all farmers have access to such additions, nor are current rates of application sustainable or environmentally desirable. Transcriptomic studies of cereal crops have revealed dramatic responses to either low N or low P single stress treatments. In the field, however, levels of both N and P may be suboptimal. The interaction between N and P starvation responses remains to be fully characterized. Results: We characterized the root and leaf transcriptomes of young maize plants grown under control, low N, low P or combined low NP conditions. We identified 1, 555 genes that responded to at least one of the treatments, in at least one of the tissues. Transcriptional responses to low N and low P were distinct, with very few genes responding in a similar way to the two single stress treatments. Furthermore, the regulation of a large group of genes was antagonistic between the two conditions. In combined NP stress, the low N response dominated, the classical P starvation response being largely suppressed. An additional experiment over a range of N availability indicated that even a mild reduction in N levels was sufficient to repress the low P induction of well-characterized P starvation genes. Although expression of P transporter genes was repressed under low N or low NP, we confirmed earlier reports of P hyper accumulation under low N. To lay the foundations for further study of the mechanistic basis of NP signaling in maize, we annotated the complete set of maize Spx genes, and found evidence of N and P regulation across the family. Conclusions: A mild reduction in N availability is sufficient to repress the P starvation response in young maize plants. The Spx gene family are candidates for integrating low N and low P responses.

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“…NRT1.1 has been found to be expressed in the epidermis-cortex and central cylinder of mature roots as well as guard cells of shoots ( Guo et al, 2003 ; Fang et al, 2020 ). Future studies should focus on the specific functions that have been ascribed to NRT1.1 in different tissues for the regulation of plant tolerance to certain environmental stresses.…”

Section: Perspectivementioning

confidence: 99%

“…Recently, nitrate transporter 1.5 (NRT1.5), initially characterised as a pH-dependent bidirectional nitrate transporter, has been shown to be involved in K + allocation in plants (Drechsler et al, 2015;. Fang et al (2020) also found that the loss of NRT1.1 in nrt1.1 mutants disturbs K + uptake and root-to-shoot allocation, resulting in greater growth arrest under low K + stress conditions. Further physiological and genetic evidence revealed that both the uptake and root-to-shoot allocation of K + in wild-type plants require the expression of NRT1.1 in the root epidermis-cortex and central vasculature.…”

Section: Low-k + Stressmentioning

confidence: 99%

“…Given that the uptake rates of NO 3 − and K + are often found to be positively correlated ( Coskun et al, 2016 ), the activity of nitrate transporters in roots may be affected by K + , as evidenced by the observation that appropriate K + supply clearly increased the expression of NRT1.1 in roots ( Xu et al, 2020 ). Notably, Fang et al (2020) revealed that these K + uptake-related interactions are dependent on an H + -consuming mechanism associated with the H + /NO 3 − symport facilitated by NRT1.1. Nevertheless, NRT1.5-involved K + loading into the xylem was verified to be only associated with its role as a proton-coupled H + /K + antiporter ( Li et al, 2017 ), which is not associated with NO 3 − transport.…”

Section: Introductionmentioning

confidence: 99%

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NRT1.1 Dual-Affinity Nitrate Transport/Signalling and its Roles in Plant Abiotic Stress Resistance

Fang

et al. 2021

Front. Plant Sci.

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NRT1.1 is the first nitrate transport protein cloned in plants and has both high- and low-affinity functions. It imports and senses nitrate, which is modulated by the phosphorylation on Thr101 (T101). Structural studies have revealed that the phosphorylation of T101 either induces dimer decoupling or increases structural flexibility within the membrane, thereby switching the NRT1.1 protein from a low- to high-affinity state. Further studies on the adaptive regulation of NRT1.1 in fluctuating nitrate conditions have shown that, at low nitrate concentrations, nitrate binding only at the high-affinity monomer initiates NRT1.1 dimer decoupling and priming of the T101 site for phosphorylation activated by CIPK23, which functions as a high-affinity nitrate transceptor. However, nitrate binding in both monomers retains the unmodified NRT1.1, maintaining the low-affinity mode. This NRT1.1-mediated nitrate signalling and transport may provide a key to improving the efficiency of plant nitrogen use. However, recent studies have revealed that NRT1.1 is extensively involved in plant tolerance of several adverse environmental conditions. In this context, we summarise the recent progress in the molecular mechanisms of NRT1.1 dual-affinity nitrate transport/signalling and focus on its expected and unexpected roles in plant abiotic stress resistance and their regulation processes.

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The K⁺ and NO₃⁻ Interaction Mediated by NITRATE TRANSPORTER1.1 Ensures Better Plant Growth under K⁺-Limiting Conditions

Cited by 23 publications

References 71 publications

Potassium and phosphorus transport and signaling in plants

Potassium and phosphorus transport and signaling in plants

Low nitrogen availability inhibits the phosphorus starvation response in maize (Zea maysssp.maysL.)

NRT1.1 Dual-Affinity Nitrate Transport/Signalling and its Roles in Plant Abiotic Stress Resistance

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