The K5 capsular polysaccharide of the bacterium Acinetobacter baumannii SDF with the same K unit containing Leg5Ac7Ac as the K7 capsular polysaccharide but a different linkage between the K units

Arbatsky, Nikolay P.; Kenyon, Johanna J.; Shashkov, Alexander S.; Shneider, Mikhail M.; Popova, Anastasiya V.; Kalinchuk, N. A.; Hall, Ruth M.; Knirel, Yu. A.

doi:10.1007/s11172-019-2432-4

Cited by 15 publications

(9 citation statements)

References 15 publications

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“…Thus, it was expected that K46 will also include an α-D-Gal p -(1→6)-α-D-Glc p NAc-(1→3)-D-Gal p NAc segment. Gtr94 K46 (GenPept accession number ENW42232.1) is 75% identical to Gtr13 K5 (GenPept accession number CAO99488.1) that has previously been shown to link Leg5Ac7Ac to D-Gal p via an α-(2→6) linkage in biosynthesis of the K5 CPS [5]. Thus, it is likely that Gtr94 K46 forms a â-Pse5Ac7Ac-(2→6)-D-Gal p linkage in K46.…”

Section: Resultsmentioning

confidence: 97%

“…baumannii isolate SDF (Fig 1A). However, in the central region, the KL46 gene cluster carries six genes, psaABCDEF , for synthesis of CMP-activated 5,7-di- N -acetylpseudaminic acid (Pse5Ac7Ac) [2] [21–23], whereas KL5 has lgaABCDEFG genes to make CMP-activated 5,7-di- N -acetyllegionaminic acid (Leg5Ac7Ac) [2][5].…”

Section: Resultsmentioning

confidence: 99%

“…K5 includes D-Gal p NAc as the first sugar of the K unit. Gtr15 then adds D-Glc p NAc via an α-(1→3) linkage to the D-Gal p NAc sugar, and Gtr14 links a D-Gal p residue via an α-(1→6) linkage to the D-Glc p NAc [5]. Thus, it was expected that K46 will also include an α-D-Gal p -(1→6)-α-D-Glc p NAc-(1→3)-D-Gal p NAc segment.…”

Section: Resultsmentioning

confidence: 99%

“…The acetylation pattern in K46 is similar to that of the K5 CPS produced by A . baumannii isolate SDF [5], which includes Leg5Ac7Ac residues that are also 4-O-acetylated (Fig 4A). Similarly, the KL5 gene cluster does not include a suitable atr gene (Fig 1).…”

Section: Resultsmentioning

confidence: 99%

“…baumannii isolate SDF was found to be 4-O-acetylated on the non-2-ulosonic acid residue, 5,7-diacetamido-3,5,7,9-tetradeoxy- D - glycero - D - galacto -non-2-ulosonic (di-N-acetyllegionaminic) acid (Leg5Ac7Ac). However, an acetyltransferase gene was not found in the KL5 gene cluster [5], suggesting that an unidentified gene located elsewhere in the genome may be involved. In this study, we examine the CPS structure produced by A .…”

Section: Introductionmentioning

confidence: 99%

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The K46 and K5 capsular polysaccharides produced by Acinetobacter baumannii NIPH 329 and SDF have related structures and the side-chain non-ulosonic acids are 4-O-acetylated by phage-encoded O-acetyltransferases

et al. 2019

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Acinetobacter baumannii isolate NIPH 329 carries a novel capsular polysaccharide (CPS) gene cluster, designated KL46, that is closely related to the KL5 locus in A . baumannii isolate SDF but includes genes for synthesis of 5,7-diacetamido-3,5,7,9-tetradeoxy- l - glycero - l - manno -non-2-ulosonic (di- N -acetylpseudaminic) acid (Pse5Ac7Ac) instead of the corresponding D - glycero - D - galacto isomer (di- N -acetyllegionaminic acid) (Leg5Ac7Ac). In agreement with the genetic content of KL46, chemical studies of the K46 CPS produced by NIPH 329 revealed a branched tetrasaccharide repeat (K unit) with an overall structure the same as K5 from SDF but with â-Pse5Ac7Ac replacing α-Leg5Ac7Ac. As for K5, the K46 unit begins with d -Gal p NAc and includes α- d -Glc p NAc-(1→3)- d -Gal p NAc and α- d -Gal p -(1→6)- d -Glc p NAc linkages, formed by Gtr14 and Gtr15 glycosyltransferases, respectively. The Gtr94 K46 glycosyltransferase, which is related to Gtr13 K5, links Pse5Ac7Ac to d -Gal p in the growing K unit via a â-(2→6) linkage. Nearly identical Wzy enzymes connect the K46 and K5 units via a α-D-Gal p NAc-(1→3)-α-D-Gal p linkage to form closely related CPSs. Both Pse5Ac7Ac in K46 and Leg5Ac7Ac in K5 are acetylated at O4 but no acetyltransferase gene is present in KL46 or KL5. Related acetyltransferases were found encoded in the NIPH 329 and SDF genomes, but not in other strains carrying an unacetylated Pse or Leg derivative in the CPS. The genes encoding the acetyltransferases were in different putative phage genomes. However, related acetyltransferases were rare among the >3000 publically available genome sequences.

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Section: Resultsmentioning

confidence: 97%