The Kampo formula “Juzen-taiho-to” exerts protective effects on ethanol-induced liver injury in mice

Abstract

-The aim of this study was to investigate whether the Japanese herbal medicine "Juzen-taiho-to (JTX)" has a protective effect on ethanol (EtOH)-induced liver injury. Seven-week-old male ICR mice were orally administered JTX or saline once a day for three days. Twenty-four hours after the last administration, the mice were intraperitoneally injected with EtOH (2 g/kg). The mice in each group were killed 24 hr after EtOH administration and were bled to obtain plasma. The mice injected with EtOH had high plasma levels of alanine aminotransferase and aspartate aminotransferase and lipid peroxidation. Histopathological examination of the liver of mice treated with EtOH revealed an abnormal outline around the central vein, glycogen depletion, and expression of prostaglandin-endoperoxide synthase 2. Pretreatment with JTX prevented the EtOH-induced increase in the levels of alanine aminotransferase and aspartate aminotransferase, lipid peroxidation, and histopathological changes. Our results suggest that JTX exerts protective effects against EtOH-induced liver disease by modulating oxidative stress and inflammatory response.

Protective effect of the Kampo formula “Juzen-taiho-to” on isoniazid- and rifampicin-induced hepatotoxicity in mice

“…Our previous investigation showed that pretreatment with JTX protects from acute hepatic injury induced by carbon tetrachloride and ethanol (Fukaya et al, 2018;. Thus, this previous study suggests that JTX may prevent several kinds of hepatic diseases.…”

“…JTX shows beneficial effects on human health and has been researched extensively as a potential candidate to prevent various diseases (Anjiki et al, 2005;Saiki, 2000). Previous investigations have shown the protective effects of JTX against CCl 4 -and ethanol-induced hepatic injury via the attenuation of oxidative stress (Fukaya et al, 2018;. In addition, JTX is reported to decrease inflammation through inhibition of the nuclear factor-kappa B (NF-κB) signaling pathway (Kawamata et al, 2000).…”

“…The supernatant was filtered through a 0.45-μm membrane filter, and high-performance liquid chromatography analysis was then performed using 10 μL of the sample. The fingerprint of chart was previously reported (Fukaya et al, 2018).…”

“…Plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were determined using the Transaminase CII Test Wako (Fujifilm Wako Pure Chemical, Osaka, Japan) according to the manufacturer's instructions as previously described (Fukaya et al, 2018). Plasma levels of interleukin (IL)-6 were determined using commercially available enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's instructions (Thermo Fisher, Waltham, MA, USA).…”

Protection from acetaminophen-induced hepatotoxicity by post-administration of 1<i>O</i>, 20<i>O</i>-diacetyl kamebakaurin in mice

“…Total malondialdehyde (MDA) levels in the liver were examined via a colorimetric microplate assay (Oxford Biochemical Research, Oxford, MI) according to the manufacturer's protocol and previously described (Fukaya et al, 2018).…”