The Karyotype of the Yellow Dung Fly,<i>Scathophaga stercoraria</i>, a Model Organism in Studies of Sexual Selection

We here argue that data from comparative studies of genome size and karyotypes provide important information for planning comparative research on genome evolution. We document for 39 species of sepsids that there is a four-fold difference in genome size (151-618 Mbp). Mapping genome sizes onto a phylogenetic hypothesis identifies that this range is the result of five genome expansions and four genome contractions that we here define as changes in genome size of more than 50 Mbp. We then generate karyotype data for 10 species and find no changes in chromosome number. The study reveals that the "Oriental" clade of sepsids is a promising system for studying genome evolution because it has experienced three genome expansion events. These events can be compared with an expansion in the "Neotropical" clade in order to reveal the mechanisms that underlie genome expansion in Sepsidae. A review of the literature on genome sizes and karyotypes reveals that they have been poorly documented in Metazoa. This means that researchers interested in the evolution of genome expansions and contractions are currently not being able to identify appropriate target taxa for genome sequencing. We thus argue for more comparative research on genome sizes and karyotypes and point out that historically species were chosen for genome sequencing for reasons not related to genome evolution (e.g. small genome size, model species status, phylogenetic position, interesting phenotypes). We believe that it is now time to use a more genome-centric selection criterion, where species for whole genome sequencing are selected based on their importance for understanding genome evolution.

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“…Fixation and Giemsa staining were carried out following a protocol by Sbilordo et al. (). The tissues were spread onto five glass slides.…”

Section: Methodsmentioning

confidence: 99%

“…The neural ganglia for each species were collected in a tube and gently minced in 1% sodium citrate solution. Fixation and Giemsa staining were carried out following a protocol by Sbilordo et al (2010). The tissues were spread onto five glass slides.…”

Section: Karyotype Analysesmentioning

confidence: 99%

Evolutionary analysis identifies multiple genome expansions and contractions in Sepsidae (Diptera) and suggests targets for future genomic research

Puniamoorthy

Özsu

et al. 2015

Cladistics

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“…Meanwhile, this fly is also a popular subject for many other studies, including sperm competition (Gress et al, 2016), sexual selection (Sbilordo et al, 2010), reproductive physiology (Reim et al, 2006), growth and development (Hosken et al, 2000;Walters et al, 2022), genetic aspects (Demont et al, 2008), thermobiology (Blanckenhorn et al, 2021), immune-related research (West and Tracy, 2009). In addition, S. stercoraria is often used for ecotoxicological tests, such as ivermectin detection (Mahdjoub et al, 2020;González-Tokman et al, 2022) The plateau pika (Ochotona curzoniae) (Lagomorpha, Ochotonidae) is a specialized native species of the Qinghai-Tibetan plateau (QTP) (Zhu et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Complete mitochondrial genome of Scathophaga stercoraria (Diptera: Scathophagidae) in wild plateau pika: genome descriptions and phylogenetic evolution

Zhang,

Chen,

Meng

et al. 2024

Parasitology

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As a member of the Scathophagidae family, Scathophaga stercoraria (S. stercoraria) is widely distributed globally and is closely associated with animal feces. It is also a species of great interest to many scientific studies. However, its phylogenetic relationships are poorly understood. In this study, S. stercoraria was found in plateau pikas for the first time. The potential cause of its presence in the plateau pikas was discussed and it was speculated that the presence of S. stercoraria was related to the yak feces. In addition, two nuclear genes (18SrDNA and 28SrDNA), one mitochondrial gene (COI), and the complete mitochondrial genome of S. stercoraria was sequenced.Phylogenetic trees constructed based on 13 Protein coding genes (13PCGs), 18S and 28S rDNA showed that S. stercoraria is closely related to the Calliphoridae family; phylogenetic results based on COI suggest that within the family Scathophagidae, S. stercoraria is more closely related to the genus Leptopa, Micropselapha, Parallelomma, and Americina. Divergence times estimated using the COI gene suggest that the divergence formation of the genus Scathophaga is closely related to changes in biogeographic scenarios and potentially driven by a combination of uplift of the Qinghai-Tibetan Plateau (QTP) and dramatic climate changes. These results provide valuable information for further studies on the phylogeny and differentiation of the Scathophaga genus in the future.

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Lista das Formigas Poneromorfas do Brasil

Feitosa¹

2015

As Formigas Poneromorfas Do Brasil

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SciELO Books / SciELO Livros / SciELO Libros FEITOSA, RM. Lista das Formigas Poneromorfas do Brasil. In: DELABIE, JHC., et al., orgs. As formigas poneromorfas do Brasil [online]. Ilhéus, BA: Editus, 2015, pp. 95-101. ISBN 978-85-7455-441-9. Available from SciELO Books . All the contents of this work, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 International license. Todo o conteúdo deste trabalho, exceto quando houver ressalva, é publicado sob a licença Creative Commons Atribição 4.0. Todo el contenido de esta obra, excepto donde se indique lo contrario, está bajo licencia de la licencia Creative Commons Reconocimento 4.0. 9 -Lista das Formigas Poneromorfas do Brasil Rodrigo M. Feitosa Lista das Formigas Poneromorfas do Brasil | 95 Resumo Com cerca de 13.000 espécies descritas em todo mundo, formigas representam o grupo mais especioso e ecologicamente diverso de insetos sociais. O Brasil, com seus seis biomas distribuídos em continentais 8.500.000 km² de território, abriga a maior diversidade de gêneros de formigas e a segunda maior diversidade de espécies do mundo. Embora estes dados sejam representativos, o conhecimento sobre a diversidade de formigas no Brasil ainda está longe de estar completo. Quando comparamos o nível de conhecimento taxonômico entre os táxons de Formicidae que ocorrem no Brasil, as subfamílias poneromorfas encontram-se em um estágio mais avançado que as demais, especialmente quando comparadas aos gêneros mais diversos de Formicinae e Myrmicinae. Neste capítulo é apresentada uma lista sinóptica das espécies de formigas poneroides e ectatomminoides que ocorrem no Brasil. FEITOSA, Rodrigo M. Lista das Formigas Poneromorfas do Brasil. In: DELABIE, Jacques H. C. et al. As formigas poneromorfas do Brasil. Ilhéus: Editus, 2015. p. 95-101. Lista das Formigas Poneromorfas do Brasil Rodrigo M. Feitosa 9 Lista das Formigas Poneromorfas do Brasil | 95

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The Karyotype of the Yellow Dung Fly,Scathophaga stercoraria, a Model Organism in Studies of Sexual Selection

Cited by 3 publications

References 40 publications

Evolutionary analysis identifies multiple genome expansions and contractions in Sepsidae (Diptera) and suggests targets for future genomic research

Evolutionary analysis identifies multiple genome expansions and contractions in Sepsidae (Diptera) and suggests targets for future genomic research

Complete mitochondrial genome of Scathophaga stercoraria (Diptera: Scathophagidae) in wild plateau pika: genome descriptions and phylogenetic evolution

Lista das Formigas Poneromorfas do Brasil

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