The Keeping Qualities of Strangles Vaccine

Bazkley, P. L.; Baldwin, Stephanie; Dickson, Helen; Thayer, J. R.

doi:10.1111/j.1751-0813.1949.tb04782.x

Cited by 5 publications

(2 citation statements)

References 4 publications

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“…Although the presence of M-like proteins in S. equi has been previously described (2,3,6,23,31,33), the information available about this molecule has been derived mostly from studies carried out with acid-extracted M protein and with culture supernatant protein. Thus, little is known about the native or nondenatured form of this molecule.…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of the M protein of Streptococcus equi and cloning and expression of the M protein gene in Escherichia coli

Galán

Timoney

1987

Infect Immun

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A Streptococcus equi gene bank was constructed in the bacteriophage Agtll cloning vector, and hybrid phage plaques were screened with S. equi M protein antiserum. A hybrid phage expressing the S. equi M protein (Xgtll/SEM7) was identified and lysogenized into Escherichia coli Y1089. The cloned M protein appeared in immunoblots as three polypeptides with relative molecular weights of 58,000, 53,000, and 50,000. When reacted with S. equi M protein antiserum in an agar double-diffusion assay, the cloned M protein formed a line of identity with a protein in an acid extract of S. equi. Furthermore, Agtl 1/SEM7 protein inhibited opsonization of S. equi by antiserum to S. equi M protein. In addition, the recombinant protein expressed determinants of the antigen in the immune complexes of purpura hemorrhagica. Native M protein obtained from S. equi and recombinant M protein showed very similar molecular weight distributions on immunoblots, appearing as multiple closely spaced bands with molecular weights ranging from 52,000 to 60,000. Antisera prepared separately against each of the acid-extracted polypeptides shown to be important in serum bactericidal responses (molecular weight, 29,000) and nasopharyngeal local antibody responses (molecular weights, 41,000 and 46,000) of the horse each reacted with all three polypeptides in an acid extract. Moreover, antisera against protoplasts and against recombinant M protein of S. equi also reacted with these polypeptides. These results suggest that the entire M protein molecule of S. equi is present in these preparations and that the fragments in acid extracts carry overlapping segments.

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Section: Discussionmentioning

confidence: 99%

Molecular analysis of the M protein of Streptococcus equi and cloning and expression of the M protein gene in Escherichia coli

Galán

Timoney

1987

Infect Immun

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“…STAMP 18 , considerando o fato dos estreptococos acidificarem progressivamente o meio de cultura, observou que nessas condições, e também quando baixa o potencial de óxido-redução, o germe elabora o enzima proteínase, cuja presença é prejudicial à antigenicidade, sendo esta observação mais um fator contra o uso de culturas incubadas por tempo prolongado. E, ainda, no caso especial de estreptococo, indicamos, de acordo com BAZELEY et al 4 , a inativação da cultura pelo car lor, já que este é o melhor método de preservar a capacidade antigênica desse germe. A vacina de estreptococos mortos pelo formol, na verificação desses autores, perde rapidamente sua eficiência em temperatura ambiente.…”

Section: -Discussões E Considerações So-bre Os Detalhes Das Técnicas Citadas Neste Trabalhounclassified

Autovacinas: técnicas de preparo e fatores de eficiência

Cury

1972

Rev. Saúde Pública

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Verificou-se que a obtenção de excelentes autovacinas é possível usando-se a técnica de semeadura, dos germes apropriados, isolados de um material clínico em meios de cultura selecionados. As condições do cultivo devem ser adequadas para estimular, ou pelo menos preservar, as qualidades antigênicas dos germes. O processo de inativação necessita ser cuidadosamente acertado, a fim de não lesar os antígenos presentes. Foram descritos métodos capazes de cumprir estes requisitos, e que ao mesmo tempo fossem práticos e relativamente simples em sua execução, com equipamentos ao alcance de qualquer laboratório clínico.

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The protective M proteins of the equine group C streptococci

Timoney

Mukhtar

1993

Veterinary Microbiology

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The Keeping Qualities of Strangles Vaccine

Cited by 5 publications

References 4 publications

Molecular analysis of the M protein of Streptococcus equi and cloning and expression of the M protein gene in Escherichia coli

Molecular analysis of the M protein of Streptococcus equi and cloning and expression of the M protein gene in Escherichia coli

Autovacinas: técnicas de preparo e fatores de eficiência

The protective M proteins of the equine group C streptococci

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