The key role of LeuRS in the development of the cleistothecium and the metabolization of the flavor during the fermentation of dark tea with Aspergillus montevidensis

Kaihui, Liu; Liu, Wanting; Ding, Xiaowei; Gao, Xiang; Lv, Jiali; Li, J.

doi:10.1016/j.lwt.2023.115188

Cited by 1 publication

(1 citation statement)

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“…PPO plays an important role in tea processing [8,9], and it determines the degree of tea oxidation. According to the degree of oxidation, tea can be divided into six categories: green tea [10] (non-oxidized), white tea [11] and yellow tea [12] (lightly oxidized), oolong tea [13] (semi-oxidized), black tea [14] (fully oxidized), and dark tea [15] (post-fermented with micro-organisms). By inhibiting or promoting the enzymatic oxidation of PPO, various categories of teas with distinct flavors are produced.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances Regarding Polyphenol Oxidase in Camellia sinensis: Extraction, Purification, Characterization, and Application

Zou,

Zhang,

et al. 2024

Foods

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Polyphenol oxidase (PPO) is an important metalloenzyme in the tea plant (Camellia sinensis). However, there has recently been a lack of comprehensive reviews on Camellia sinensis PPO. In this study, the methods for extracting PPO from Camellia sinensis, including acetone extraction, buffer extraction, and surfactant extraction, are compared in detail. The main purification methods for Camellia sinensis PPO, such as ammonium sulfate precipitation, three-phase partitioning, dialysis, ultrafiltration, ion exchange chromatography, gel filtration chromatography, and affinity chromatography, are summarized. PPOs from different sources of tea plants are characterized and systematically compared in terms of optimal pH, optimal temperature, molecular weight, substrate specificity, and activators and inhibitors. In addition, the applications of PPO in tea processing and the in vitro synthesis of theaflavins are outlined. In this review, detailed research regarding the extraction, purification, properties, and application of Camellia sinensis PPO is summarized to provide a reference for further research on PPO.

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