The kinetics of local cytokine and galectin expression after challenge infection with the gastrointestinal nematode, Haemonchus contortus

Robinson, Nicholas A.; Pleasance, Jill; Piedrafita, David; Meeusen, Elza Nicole Theresia

doi:10.1016/j.ijpara.2010.11.006

Cited by 43 publications

(47 citation statements)

References 32 publications

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“…Recognition proteins such as lectins are involved in the immune response against nematodes (MEEUSEN et al, 2005). In particular, members of the galectin and the C-type lectin family have been identified in the abomasum of cattle and sheep infected with Ostertagia ostertagi and Cooperia oncophora (DUNPHY et al, 2000;LI & GASBARRE, 2009;LI et al, 2010;ROBINSON et al, 2011). Collectins belong to a mammalian superfamily of the lectin C-type protein subgroup with the ability to recognize pathogen-associated molecular patterns (PAMPs) found on the surface of microorganisms (KILPATRICK, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…This suggests that there is activation of the alternative complement pathway and early reactivation in secondary infections (LI et al, 2010). Another response mechanism observed in H. contortus-infected sheep is characterized by abundant secretion of galectin-11 in the abomasum, increased mucus production (DUNPHY et al, 2000) and alteration of mucus viscosity, thus providing an unfavorable environment for parasite survival (ROBINSON et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Souza

Lambert

Nishi

et al. 2015

Rev. Bras. Parasitol. Vet.

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Galectins and collectins are proteins classified in the lectin family that have the ability to recognize molecular patterns associated with pathogens. Studies on cattle have demonstrated high expression of these proteins during infection with gastrointestinal nematodes. The aim of this study was to investigate whether the level of Haemonchus contortus infection would alter the expression of galectins (Gal11 and Gal14) and collectins (SPA and CGN) in sheep. Twelve Corriedale sheep exposed to natural infection with nematodes were divided into two groups: group 1 (G1, n = 7) and group 2 (G2, n = 5), with low and high parasite burdens, respectively, based on fecal egg counts and abomasal parasite counts. The fecal egg counts and abomasal parasite counts were significantly different (p < 0.05) between the groups. Galectin and collectin gene expression was observed in all sheep abomasal samples. However, animals with lower infection levels showed lower expression of the genes Gal14, SPA and CGN (p < 0.05). Expression of lectins was associated with the abomasal H. contortus burden, thus suggesting that these proteins may have a role in controlling of this infection.Keywords: Innate immunity, lectins, helminths, small ruminants, PCR. ResumoColectinas e galectinas são proteínas da família das lectinas que possuem a capacidade de reconhecer padrões moleculares associados aos patógenos. Estudos em bovinos têm demonstrado a alta expressão dessas proteínas durante a infecção por nematoides gastrintestinais. O objetivo deste estudo foi investigar se o nível de infecção de Haemonchus contortus altera a expressão de colectinas (SPA e CGN) e galectinas (Gal11 e Gal14) de ovinos. Doze ovinos da raça Corriedale expostos a infecção natural com nematoides foram separados em dois grupos: grupo 1 (G1, n=7) com menor grau de parasitismo; e grupo 2 (G2, n=5) com maior grau, a partir da contagem do número de parasitos recuperados do abomaso e OPG. A contagem de OPG e de parasitos recuperados do abomaso dos grupos G1 e G2 apresentaram diferença estatística (p<0,05). A expressão dos genes de colectinas e galectina foi observada em todas as amostras de abomaso dos ovinos, porém animais com menor grau de infecção apresentaram menor expressão dos genes de Gal14, SPA e CGN (p<0,05). A expressão de lectinas foi associada ao número de H. contortus encontrados no abomaso de ovinos, indicando um possível papel dessas proteínas no controle da infecção.Palavras-chave: Imunidade inata, lectinas, helmintos, pequenos ruminantes, PCR.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Souza

Lambert

Nishi

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…Analysis of H. contortus infected sheep demonstrated release of LGALS-14 into the gastrointestinal mucus, the interface of host and parasite interaction (Dunphy et al 2002). In addition, kinetic studies of LGAL-14 showed that release into the mucus occurred soon after challenge infection, and correlated with a reduction in parasitic burden (Robinson et al 2011). …”

Section: Ietroductioementioning

confidence: 99%

“…Analysis of the mucosal contents lining the gastrointestinal tract also revealed secretion of LGALS-11 into the mucus. An observation of increased mucus stickiness corresponding with the production of LGALS-11 suggested that LGALS-11 might work by interacting with the mucus to impede H. contortus motility (Robinson et al 2011). Recent immunofluorescent staining techniques using a recombinant form of galectin-11 have revealed binding to the fourth larval stage and adult H.…”

Section: Ietroductioementioning

confidence: 99%

“…The finding that LGAL-14 is concentrated within eosinophils (an immune cell considered a major mediator of parasite killing, including of H. contortus) suggested the possibility of a direct role for ruminant galectins in mediating parasite-killing (Meeusen & Balic 2000;Robinson et al 2011). The subsequent demonstration of direct binding of LGAL-11 to H. contortus and their ability to inhibit larval development and growth hn vhtro has confirmed the roles of galectins and ability to directly kill relatively large multicellular pathogens (Preston et al 2015b).…”

Section: Coeclusioementioning

confidence: 99%

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Proteomic identification of Galectin-11 and 14 ligands from Haemonchus contortus

Sakthivel

Swan

Preston

et al. 2017

Preprint

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Haemonchus contortus is the most pathogenic nematode of small ruminants. Infection in sheep and goats results in anaemia that decreases animal productivity and can ultimately cause death. The involvement of ruminant-specific galectin-11 (LGALS-11) and galectin-14 (LGALS-14) has been postulated to play important roles in protective immune responses against parasitic infection; however, their ligands are unknown. In the current study,LGALS-11 and LGALS-14 ligands in H. contortus were identified from larval (L4) and adult parasitic stages extracts using immobilised LGALS-11 and LGALS-14 affinity column chromatography and mass spectrometry. AbstractHaemonchus contortus is the most pathogenic nematode of small ruminants. Infection in sheep and goats results in anaemia that decreases animal productivity and can ultimately cause death.The involvement of ruminant-specific galectin-11 (LGALS-11) and galectin-14 (LGALS-14) has been postulated to play important roles in protective immune responses against parasitic infection; however, their ligands are unknown. In the current study, ligands in H. contortus were identified from larval (L4) and adult parasitic stages extracts using immobilised LGALS-11 and LGALS-14 affinity column chromatography and mass spectrometry.

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Evaluation of the Role of Galectins in Parasite Immunity

Swan

Sakthivel

Beddoe

et al. 2022

Methods in Molecular Biology

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The kinetics of local cytokine and galectin expression after challenge infection with the gastrointestinal nematode, Haemonchus contortus

Cited by 43 publications

References 32 publications

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Proteomic identification of Galectin-11 and 14 ligands from Haemonchus contortus

Evaluation of the Role of Galectins in Parasite Immunity

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