The knockdown of the maternal estrogen receptor 2a (esr2a) mRNA affects embryo transcript contents and larval development in zebrafish

Celeghin, Andrea; Benato, Francesca; Pikulkaew, Surachai; Rabbane, Md. Golam; Colombo, Lorenzo; Valle, Luisa Dalla

doi:10.1016/j.ygcen.2010.12.020

Cited by 31 publications

(23 citation statements)

References 36 publications

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“…Although important, this approach lacks the power to investigate the changes in deposition of non-lethal transcripts, which could have milder but important and persistent effects on fitness. For example, morpholino knockdown of maternal transcripts for the oestrogen receptor 2a negatively affected embryonic and larval development in zebrafish (Celeghin et al, 2011). Maternally deposited transcripts of radar, a TGF- signalling molecule, were shown to be essential for the ventral fate of cells during development, with loss-of-function causing lethal dorsalized phenotypes (Sidi et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Experimental selection for body size at age modifies early-life history traits and muscle gene expression in adult zebrafish

Amaral

Johnston

2012

Journal of Experimental Biology

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SUMMARYThe short generation time of the zebrafish (Danio rerio) was exploited to investigate the effects of selection for body size at age on early life-history traits and on the transcriptional response to a growth stimulus in skeletal muscle of adult fish. Replicate populations were either unselected (U-lineage) or subjected to four generations of experimental selection for small (S-lineage) or large (L-lineage) body size at 90days post-fertilization. Body mass was on average 16.3% and 41.0% higher in the L-than in the U-and S-lineages, respectively. Egg diameter was 6.4% lower with 13% less yolk in the S-lineage compared with the other lineages. Maternal transcripts for igf2r, bmpr1aa, igf1ar, igf2a, igfbp5a, ghra and igfbp3 in 2-4 cell stage embryos were higher in the L-than in the S-lineage. Larvae from the L-lineage were significantly larger, but survivorship at the end of the first month was similar between lineages. Gene expression was measured in the fast muscle of adult fish fasted for 7days and then re-fed to satiation for 48h. The expression of 11 insulin-like growth factor pathway genes and 12 other nutritionally responsive genes was similar for the S-and L-lineages as was gut fullness with feeding. Transcript abundance for four genes (igf1a, igf2r, igfbp1a and igfbp1b) showed either regulated or constitutive differences between the S-and L-lineages. For example, igf2 receptor transcript abundance was higher and igbp1a/b transcript abundance was lower in the L-than in the S-lineage, consistent with an effect of selection on insulin-like growth factor signalling. Supplementary material available online at

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Section: Discussionmentioning

confidence: 99%

Experimental selection for body size at age modifies early-life history traits and muscle gene expression in adult zebrafish

Amaral

Johnston

2012

Journal of Experimental Biology

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“…12 anx2a plays vital role as calcium ion binding, calcium-dependent phospholipid binding and cytoskeletal protein binding. [12][13][14] So these binding capacities could be reduced by down-regulating anx2a gene expression. ccng1 transcripts for all treatments were strongly and equally expressed at 8 hpf stage embryos (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

Effect of Maternal Estradiol Hormone on Gene Expression in Zebrafish (Danio rerio) Embryos and its Clinical Implications

Rabbane

Rahman

2014

Dhaka Univ. J. Pharm. Sci

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Maternal steroid hormone, estradiol plays significant role in fertilized eggs by performing proper embryo development. The study was performed to observe the genetic effects of estradiol by using reverse transcriptase polymerase chain reaction. A total three steroid responsive genes were analysed namely anx2a, ccng1and hmox1. The ef1a gene was used as reference to normalize data. The transcripts of anx2a were down regulated in E2 0.01 treated embryos at 8 hpf when compared to contol and E2 0.1 treated embryos. The ccng1 transcripts for E2 0.1 and E2 0.01 and E2 treated embryos were equally and strongly expressed. There was also no up or downregulation when analysed with hmox1 gene. So the results suggest that different estradiol concentrations can alter specific genetic expression at 8 hpf embryonic state and clinically it could be used as an effective gene therapy.However, extensive studies should be conducted to assess its beneficial effects on human being.

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“…Temporary knockdown by a morpholino of the ERβ2 expression resulted in the disrupted development of these neuromasts (absence of hair cells), which could be related to an aberrant activation of the Notch signaling pathway in the morpholinotreated embryos (Froehlicher et al 2009). Knockdown of ERα expression during embryonic stages by morpholinoinduced blocking of translation of maternal transcripts resulted in severe developmental defects and early mortality (Celeghin et al 2011). Induction of specific target genes of ERα and ERβ2 could also be inhibited by morpholino knockdown of the receptor (Griffin et al 2013).…”

Section: The Zebrafish Estrogen Receptors (Ers)mentioning

confidence: 99%

Nuclear receptor research in zebrafish

Schaaf

2017

Journal of Molecular Endocrinology

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Nuclear receptors (NRs) form a superfamily of transcription factors that can be activated by ligands and are involved in a wide range of physiological processes. NRs are well conserved between vertebrate species. The zebrafish, an increasingly popular animal model system, contains a total of 73 NR genes, and orthologues of almost all human NRs are present. In this review article, an overview is presented of NR research in which the zebrafish has been used as a model. Research is described on the three most studied zebrafish NRs: the estrogen receptors (ERs), retinoic acid receptors (RARs) and peroxisome proliferator-activated receptors (PPARs). The studies on these receptors illustrate the versatility of the zebrafish as a model for ecotoxicological, developmental and biomedical research. Although the use of the zebrafish in NR research is still relatively limited, it is expected that in the next decade the full potential of this animal model will be exploited.

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The knockdown of the maternal estrogen receptor 2a (esr2a) mRNA affects embryo transcript contents and larval development in zebrafish

Cited by 31 publications

References 36 publications

Experimental selection for body size at age modifies early-life history traits and muscle gene expression in adult zebrafish

Experimental selection for body size at age modifies early-life history traits and muscle gene expression in adult zebrafish

Effect of Maternal Estradiol Hormone on Gene Expression in Zebrafish (Danio rerio) Embryos and its Clinical Implications

Nuclear receptor research in zebrafish

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