The Laboratory Diagnosis of <i>Haemophilus ducreyi</i>

Alfa, Michelle J.

doi:10.1155/2005/851610

Cited by 32 publications

(18 citation statements)

References 21 publications

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“…ducreyi [22]. If vancomycin-susceptible strains are suspected [23], additional use of unsupplemented media may be considered, with the caveat that this would double the cost of cultures and may prove to be impractical in resource-poor areas [24] 2) The phylogenetic tree based on dsrA - hgbA - ncaA sequences is similar to that based on the whole-genome sequences. Therefore, dsrA - hgbA - ncaA -based multilocus sequence typing could be reliably used for epidemiological investigation of CU and GU strains.…”

Section: Discussionmentioning

confidence: 99%

Haemophilus ducreyi Cutaneous Ulcer Strains Diverged from Both Class I and Class II Genital Ulcer Strains: Implications for Epidemiological Studies

Gangaiah

Spinola

2016

PLoS Negl Trop Dis

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BackgroundHaemophilus ducreyi has emerged as a major cause of cutaneous ulcers (CU) in yaws-endemic regions of the tropics in the South Pacific, South East Asia and Africa. H. ducreyi was once thought only to cause the genital ulcer (GU) disease chancroid; GU strains belong to 2 distinct classes, class I and class II. Using whole-genome sequencing of 4 CU strains from Samoa, 1 from Vanuatu and 1 from Papua New Guinea, we showed that CU strains diverged from the class I strain 35000HP and that one CU strain expressed β-lactamase. Recently, the Center for Disease Control and Prevention released the genomes of 11 additional CU strains from Vanuatu and Ghana; however, the evolutionary relationship of these CU strains to previously-characterized CU and GU strains is unknown.Methodology/Principal FindingsWe performed phylogenetic analysis of 17 CU and 10 GU strains. Class I and class II GU strains formed two distinct clades. The class I strains formed two subclades, one containing 35000HP and HD183 and the other containing the remainder of the class I strains. Twelve of the CU strains formed a subclone under the class I 35000HP subclade, while 2 CU strains formed a subclone under the other class I subclade. Unexpectedly, 3 of the CU strains formed a subclone under the class II clade. Phylogenetic analysis of dsrA-hgbA-ncaA sequences yielded a tree similar to that of whole-genome phylogenetic tree.Conclusions/SignificanceCU strains diverged from multiple lineages within both class I and class II GU strains. Multilocus sequence typing of dsrA-hgbA-ncaA could be reliably used for epidemiological investigation of CU and GU strains. As class II strains grow relatively poorly and are relatively more susceptible to vancomycin than class I strains, these findings have implications for methods to recover CU strains. Comparison of contemporary CU and GU isolates would help clarify the relationship between these entities.

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Section: Discussionmentioning

confidence: 99%

Haemophilus ducreyi Cutaneous Ulcer Strains Diverged from Both Class I and Class II Genital Ulcer Strains: Implications for Epidemiological Studies

Gangaiah

Spinola

2016

PLoS Negl Trop Dis

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“…Chancroid is diagnosed with gram staining, culture or PCR (most sensitive test) (11). Treatment is with single dose oral 1g azithromycin or 250 mg IM ceftriaxone or 500mg ciprofloxacin twice a day for 3 days.…”

Section: Chancroid (Haemophilus Ducreyi)mentioning

confidence: 99%

A Brief Review of Viral and Bacterial Sexually Transmitted Diseases in Colorectal Practice

Nabi¹

2015

Ann Colorectal Res

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Context: Sexually transmitted diseases (STDs) are a common source of presentation to colorectal surgeons. Clinicians need to remain mindful of the possibility of STDs when faced with atypical clinical presentations. This article aims to provide surgeons with a synopsis of common pathogens, their clinical presentations, diagnostic investigations and treatment regimens. Evidence Acquisition: The most common bacterial pathogens include Chlamydia trachomatis and Neisseria gonorrhea with synchronous infections at presentation occurring frequently. Patients often present with proctitis. Gonorrhea patients can also experience bloody purulent perianal discharge. Less common bacterial pathogens include syphilis, chancroid and donovanosis. The commonest STD worldwide remains human papillomavirus. Given its vast array of subtypes its manifestations include benign hyperproliferative lesions like perianal warts and extend to anal intraepithelial neoplasia and squamous cell carcinoma. Other important viral infections of the anorectum include human immunodeficiency virus and subsequent acquired immune deficiency disease as well as herpes simplex virus and molluscum contangiosum. Results: Debate exists whether the increasing incidence of STDs affecting the anorectum reported in western literature represents a real increase or a reflection of greater patient and clinician recognition and reporting. Conclusions: Regardless, a broad understanding of common bacterial and viral pathogens remains important part of modern colorectal practice. Remaining mindful of the manifestations of these common pathogens, options for diagnosis and management are important in disease control to limit the impact of these pathogens across the wider community.

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“…La culture est complexe, et la sensibilité même en condition optimale n'est que de 80 %. Les techniques de PCR permettent d'améliorer le rendement diagnostique mais ne sont pas disponibles partout [18].…”

Section: Biologieunclassified

Ulcérations anales des infections sexuellement transmissibles

Roumeguère

2009

Colon Rectum

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L'ulcération anale est une forme fréquente d'infection sexuellement transmissible (IST). La principale étio-logie en France est l'herpès. D'autres étiologies doivent être évoquées, notamment en cas de sexualité à risque et/ou chez les patients issus de zone d'endémie de certaines IST : syphilis, lymphogranulomatose vénérienne (LGV), chancre mou, donovanose, amibiase. Le diagnostic repose sur le contexte de rapport sexuel à risque, l'aspect des lésions, associés aux prélèvements bactériens et sérologiques. L'association de ces infections n'est pas rare. Il faut également rechercher une infection par le VIH, car l'augmentation récente de l'incidence de ces IST a été observée essentiellement dans la population homosexuelle masculine ayant une sexualité à risque non protégée. De plus, les ulcérations anales augmentent le risque de transmission et de contage du virus VIH. Enfin, la co-infection par le VIH pose des problèmes diagnostiques (les lésions anales étant parfois atypiques) et des problèmes thérapeutiques avec des infections souvent plus difficiles à traiter. Le traitement est probabiliste dans l'attente des résultats biologiques et doit prendre en compte les partenaires sexuels.Mots clés Ulcération anale · Infection sexuellement transmissible · Herpès · Syphilis · Lymphogranulomatose vénérienne · Chancre mou · Donovanose · Amibiase Les cinq points utiles à la thérapeutique -L'herpès est la première cause d'ulcération anale, et la syphilis doit systématiquement être évoquée devant une ulcéra-tion anale ;-toutes les ulcérations anales augmentent le risque d'infection par le VIH. Une sérologie VIH, répétée à trois mois en cas de négativité initiale, doit être proposée ;-un dépistage de la syphilis doit être proposé régulièrement dans les populations à risque ;-le traitement de la primo-infection herpétique par aciclovir ou valaciclovir diminue la durée et l'intensité des symptômes, et potentiellement le risque de transmission du VIH ;-le traitement d'une ulcération d'allure non herpétique associe dans tous les cas celui de la syphilis à celui de la LGV en cas de population à risque ou au traitement du chancre mou en cas de séjour ou d'origine de pays d'endémie.

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The Laboratory Diagnosis of Haemophilus ducreyi

Cited by 32 publications

References 21 publications

Haemophilus ducreyi Cutaneous Ulcer Strains Diverged from Both Class I and Class II Genital Ulcer Strains: Implications for Epidemiological Studies

Haemophilus ducreyi Cutaneous Ulcer Strains Diverged from Both Class I and Class II Genital Ulcer Strains: Implications for Epidemiological Studies

A Brief Review of Viral and Bacterial Sexually Transmitted Diseases in Colorectal Practice

Ulcérations anales des infections sexuellement transmissibles

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