The laboratory germination of crisp lettuce seeds under moisture stress

Abstract

Seeds of the crisp lettuce cultivar Pennlake were germinated using all combinations of six 'initial' solutions of polyethylene glycol 6000 (PEG) with osmotic potentials ranging from 0 to -8 bars and seven 'secondary' solutions of PEG with osmotic potentials ranging from 0 to -10 bars, to which seeds were moved after 24 or 48 h in the 'initial' solution. The number of seeds germinating decreased at more negative osmotic potentials of both 'initial' and 'secondary' solutions but there was an interaction between germination temperature and the osmotic potential of the 'initial' solution. At an 'initial' solution osmotic potential of 0 bars germination at 20 "C exceeded that at 10 "C. As the osmotic potential of the 'initial' solution decreased germination at 20 "C decreased more than at 10 "C so that at the more negative osmotic potentials germination at 10 "C exceeded that at 20 "C. However seeds ungerminated after 14 days germinated normally when transferred back to water, so that the average final germination was 99.5%.The results suggest that major fluctuations in soil water potential in a seedbed are unlikely to influence seed germination per se provided that a period of 24 to 48 h at 0 bars tension is available at some time. The timing of such a period relative to sowing will have a considerable effect on the time of germination and hence the time of emergence. It is concluded that factors other than the direct effect of soil moisture content on germination are involved in reducing seedling emergence under fluctuating soil moisture conditions in the field. 49-57. HEYDECKER, W. & GIBBINS, B. M. (1978). Attempts to synchronise seed germination. Acta Horticulfurae 72, 79-92. Transactions of the American Society of Agricultural Engineers 18, 105 1-1056. different soil moisture conditions. Plant Physiology 18, 524-529. 78, 349-359. Lettuce germination 453 KAUFMANN, M. R. (1969). Effects of water potential on germination of lettuce, sunflower and citrus seeds.